Host/IsotypeMouse / IgG2b
Clone27B10.4
Species ReactivityAll species
Anti-phosphotyrosine-HRP antibody is an HRP labeled tyrosine phosphorylation specific antibody that is part of the Signal-Seeker™ product line. APY03-HRP phosphotyrosine antibody recognizes a wide range of tyrosine phosphorylated proteins in cells treated with kinase inhibitors, hydrogen peroxide (H2O2) and sodium vanadate (Figure 1) and has been validated for western blot applications. APY03 anti-phosphotyrosine antibody 27B10 has been tested in western blot, immunofluorescence, immunoprecipitation, and ELISA with excellent specificity and low background with strong potent signals.
APY03 is a mouse monoclonal antibody 27B10 that recognizes phosphorylated tyrosine residues. APY03 was raised against a proprietary mixture of phosphotyrosine peptides conjugated to KLH. APY03 can detect less than 10 ng of phosphotyrosine-labeled bovine serum albumin (see Certificate of Analysis [COA]). APY03 is purified by protein G affinity chromatography and is supplied as a lyophilized white powder. Each Lot of antibody is quality controlled to provide a high batch to batch consistency. The Lot specific µg per tube can be found in the Lot specific COA documents. APY03-HRP shows high specificity to phosphotyrosine proteins and does not cross-react with phosphoserine/ threonine proteins a western blot assay (Figures 1).
Figure 1: Western Blot demonstration of APY03-HRP phosphotyrosine specificity
A431 cells were either treated (+) or untreated (-) with Calyculin A (CalyA: a serine/threonine phosphatase inhibitor, 50nM for 1 hour). NIH3T3 cells were either treated or untreated with H2O2-activated sodium orthovanadate (Vanadate: a specific tyrosine phosphatase inhibitor, 100 µM for 10 minutes). 10 µg of each lysate was resolved in SDS-PAGE and proteins were transferred to PVDF membrane. APY03-HRP (1:6000) was used to detect tyrosine phosphorylated proteins. Western blot was developed with SuperSignal West Dura chemiluminescent reagent (Thermo Scientific) and exposure time was 10 seconds. As shown in Fig.1, a wide range of tyrosine phosphorylated proteins were detected in NIH3T3 cells treated with orthovanadate but not in Calyculin A treated A431 cells.
To see the full Western blot protocol, see the product datasheet.
For more information contact: signalseeker@cytoskeleton.com
Associated Products:
Signal-Seeker™ Phosphotyrosine Detection Kit (Cat. # BK160)
Signal-Seeker™ Phosphotyrosine Affinity Beads (Cat.# APY03-beads)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
For product Datasheets and MSDSs please click on the PDF links below.
Sample Size Datasheet (Cat. APY03-HRP-S): 
Certificate of Analysis: Lot 011
For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
