sumo-2-3-antibody-asm24
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Anti-SUMO-2/3 antibody is a mouse monoclonal antibody. The antibody was raised against full-length recombinant SUMO-2 protein (Uniprot: P61956) combined with a proprietary mix of peptides that include CQIRFRFDGQPINE. The antibody has been shown to immunoprecipitate a wide range of SUMO-2/3 targeted proteins in a HeLa cell lysate (Fig. 1A). A linear epitope has not been identified and ASM24 appears to recognize a conformational epitope. Each Lot of antibody is quality controlled to provide a high batch to batch consistency. The Lot specific µg per tube can be found in the Lot specific COA documents at www.cytoskeleton.com. ASM24 is purified by Protein G affinity chromatography and is supplied as a lyophilized white powder.
Each Lot of antibody is quality controlled to provide a high batch to batch consistency. The Lot specific µg per tube can be found in the Lot specific COA documents.
Figure 1: Immunoprecipitation using SUMO-2/3 Antibody Denatured cell lysates were prepared from HS43, CT37 and KD S210 (HS43: Heat Shock treated (43°C for 10min), CT37: untreated and KD S2: shRNA SUMO-2 knock down). 1mg of lysate was used for the immunoprecipitation of SUMO-2/3 conjugates. IP experiments were performed by the protocol presented in IP and WB Method. Western blots of immunopre-cipitated proteins were developed using anti-SUMO-2/3 (Cytoskeleon cat# ASM23) (A) or anti-TFII-I antibody (B). (A) Star (*) and circle (o) indicate heavy and light chains of antibodies. Un-conjugated free SUMO is denoted by a triangle. (B) Unconjugated TFII-I is visible near 120 kDa. Multiple bands indicate that TFII-I is SUMOylated by several SUMO-2/3 proteins. TFII-I has previously been reported to be a target for Sumoylation 10,11. To see the full Immunoprecipitation protocol, see the product datasheet. |
HeLa cells were stained and visualized by widefield fluorescence microscopy as de-scribed in the IF method below. The cells were stained against α/β-tubulin (sheep anti-tubulin Ab, Cat# ATN02, green) and SUMO-2/3 (11G2, red). DNA was stained with DAPI. Mitotic cells in metaphase were imaged with a Zeiss Axio Observer.Z1 microscope (1.4 NA 63X objective). The localization of SUMO 2/3-conjugates at chromosomes can be observed during mitosis as has been previously reported12. To see the full Immunofluorescence protocol, see the product datasheet.
Figure 3: Immunofluorescence of HeLa cells in interphase with SUMO-2/3 Antibody
HeLa cells were stained and visualized by widefield fluorescence microscopy as de-scribed in the IF method below. The cells were stained against β-tubulin (sheep anti-tubulin Ab, Cat# ATN02, green) and SUMO-2/3 (11G2, red). DNA was stained with DAPI. Cells in interphase were imaged with a Zeiss Axio Observer.Z1 microscope (1.4 NA 63X objective). PML nuclear bodies (nuclear dots) were visible in SUMO-2/3 staining as has been previously reported5. To see the full Immunofluorescence protocol, see the product datasheet.
For more information contact: signalseeker@cytoskeleton.com
Associated Products:
Signal-Seeker™ SUMOylation 2/3 Detection Kit (Cat. # BK162)
Signal-Seeker™ SUMOylation 2/3 Affinity Beads (Cat.# ASM24-beads)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
For product Datasheets and MSDSs please click on the PDF links below.
Sample Size Datasheet (Cat. ASM24-S): 
Certificate of Analysis: Lot 011
For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
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去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
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你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
