Host/IsotypeMouse/IgG1-kappa Clone11G2 SpeciesReactivityBroadReactivity ValidationDataWhitePaperAntibodypedia | Anti-SUMO-2/3antibodyisamousemonoclonalantibody.Theantibodywasraisedagainstfull-lengthrecombinantSUMO-2protein(Uniprot:P61956)combinedwithaproprietarymixofpeptidesthatincludeCQIRFRFDGQPINE.TheantibodyhasbeenshowntoimmunoprecipitateawiderangeofSUMO-2/3targetedproteinsinaHeLacelllysate(Fig.1A).AlinearepitopehasnotbeenidentifiedandASM24appearstorecognizeaconformationalepitope.EachLotofantibodyisqualitycontrolledtoprovideahighbatchtobatchconsistency.TheLotspecificµgpertubecanbefoundintheLotspecificCOAdocumentsatwww.Cytoskeleton.com.ASM24ispurifiedbyProteinGaffinitychromatographyandissuppliedasalyophilizedwhitepowder. EachLotofantibodyisqualitycontrolledtoprovideahighbatchtobatchconsistency.TheLotspecificµgpertubecanbefoundintheLotspecificCOAdocuments. |
ValidatedApplications
Figure1:ImmunoprecipitationusingSUMO-2/3Antibody DenaturedcelllysateswerepreparedfromHS43,CT37andKDS210(HS43:HeatShocktreated(43°Cfor10min),CT37:untreatedandKDS2:shRNASUMO-2knockdown).1mgoflysatewasusedfortheimmunoprecipitationofSUMO-2/3conjugates.IPexperimentswereperformedbytheprotocolpresentedinIPandWBMethod.Westernblotsofimmunopre-cipitatedproteinsweredevelopedusinganti-SUMO-2/3(Cytoskeleoncat#ASM23)(A)oranti-TFII-Iantibody(B).(A)Star(*)andcircle(o)indicateheavyandlightchainsofantibodies.Un-conjugatedfreeSUMOisdenotedbyatriangle.(B)UnconjugatedTFII-IisvisIBLenear120kDa.MultiplebandsindicatethatTFII-IisSUMOylatedbyseveralSUMO-2/3proteins.TFII-IhaspreviouslybeenreportedtobeatargetforSumoylation10,11.ToseethefullImmunoprecipitationprotocol,seetheproductdatasheet. | |
Figure2:ImmunofluorescenceofHeLacellsinmetaphasewithSUMO-2/3Antibody HeLacellswerestainedandvisualizedbywidefieldfluorescencemicroscopyasde-scribedintheIFmethodbelow.Thecellswerestainedagainstα/β-tubulin(sheepanti-tubulinAb,Cat#ATN02,green)andSUMO-2/3(11G2,red).DNAwasstainedwithDAPI.MitoticcellsinmetaphasewereimagedwithaZeissAxioObserver.Z1microscope(1.4NA63Xobjective).ThelocalizationofSUMO2/3-conjugatesatchromosomescanbeobservedduringmitosisashasbeenpreviouslyreported12.ToseethefullImmunofluorescenceprotocol,seetheproductdatasheet. | |
Figure3:ImmunofluorescenceofHeLacellsininterphasewithSUMO-2/3Antibody HeLacellswerestainedandvisualizedbywidefieldfluorescencemicroscopyasde-scribedintheIFmethodbelow.Thecellswerestainedagainstβ-tubulin(sheepanti-tubulinAb,Cat#ATN02,green)andSUMO-2/3(11G2,red).DNAwasstainedwithDAPI.CellsininterphasewereimagedwithaZeissAxioObserver.Z1microscope(1.4NA63Xobjective).PMLnuclearbodies(nucleardots)werevisibleinSUMO-2/3stainingashasbeenpreviouslyreported5.ToseethefullImmunofluorescenceprotocol,seetheproductdatasheet. |
Formoreinformationcontact:signalseeker@cytoskeleton.com
AssociatedProducts:
Signal-Seeker™SUMOylation2/3DetectionKit(Cat.#BK162)
Signal-Seeker™SUMOylation2/3AffinityBeads(Cat.#ASM24-beads)
Signal-Seeker™:BlastR™RapidLysatePrepKit(Cat.#BLR01)
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以下来自摆渡百科
细胞在发生凋亡时,会激活一些DNA内切酶,这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测,可以发现180-200bp的DNA ladder。基因组DNA断裂时,暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ,从而可以通过荧光显微镜或流式细胞仪进行检测,这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
第二个问题:兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”,如果对自己的蛋白产生免疫反应,那就麻烦了。
可以类比器官移植,亲缘关系越近,越不容易产生免疫排斥。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息,你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药,那工作人员说不是抗生素怎么消炎呢,请问知情网友求一替代品,谢了。
你看这个抗体的质量怎么样,说明里面有没有说可以做组化,还是只能做western blot。