Host/IsotypeMouse/IgG1 CloneP4D1 SpeciesReactivityWiderangeofspecies | Anti-ubiquitinantibodyisapan-ubiquitin,mousemonoclonalantibodythatispartoftheSignal-Seeker™productline.Theantibodywasraisedagainstfulllengthbovineubiquitin.Theantibodyhasbeenshowntorecognizepoly-ubiquitin,mono-ubiquitin,andfreeubiquitin(Fig1).UbiquitinisahighlyconservedproteinandAUB01ispredictedtorecognizeubiquitinfromawiderangeofspecies(5).AUB01ispurifiedbyProteinGaffinitychromatographyandissuppliedasalyophilizedwhitepowder.
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ValidatedApplications
WesternBlotusingUbiquitinAntibody Anti-UbiquitinAntibody(Cat.#AUB01)wasusedata1:500dilution.Bovinethymusubiquitinwasrunasfollows;Lane1-50ng,Lane2-25ng.Lane3–12.5ng,Lane4-6.25ng,Lane5-3.12ng.Lanes6&7represent20µgofSwiss3T3celllysatefromcellstreatedfor5hwith10µMMG132(Lane6)oruntreatedcells(Lane7).Arrowindicatesfreeubiquitinband(8kD),highermolecularweightbandsareubiquitinatedproteins.ToseethefullWesternblotprotocol,seetheproductdatasheet. |  |
ImmunofluorescenceUbiquitinAntibody HeLacellswerestainedandvisualizedbyfluorescencemicroscopyasdescribedintheIFmethodbelow.UbiquitintargetedcytoplasmicandnuclearproteinsandfreeubiquitinwerestainedusingAUB01at1:500dilution.ToseethefullImmunofluorescenceprotocol,seetheproductdatasheet. |  |
UbiquitinBackground
Ubiquitin(Ub)andubiquitin-likeproteins(Ubls,e.g.SUMO,Nedd)areagroupofapproximately15proteinsthathaveamolecularweightofaround8kD.Duringtheubiquitinationprocess,theseareconjugatedviaactivating(E1),conjugating(E2)andligating(E3)enzymestolysinesofatargetprotein(1).Mammaliancellsexpressover600potentialubiquitinligaseswhichexceedsthatofthekinasesuperfamilyofPTMproteins(2).
Onefunctionofubiquitinationistotargetproteinsforproteosomaldegradation.Thisrolecanrangefromageneralhousekeepingfunctionthatclearsmissfoldedproteinsfromacelltoinvolvementintightlyregulatedspatio-temporalcellsignalingevents(1).AnemergingfunctionofubiquitinationisitsABIlitytoactivateproteinsviathecreationofuniqueprotein:proteininteractions(3).IncommonwithmanyotherPTMs,ubiquitinationisreversIBLe.Ubiquitin-specificproteases(USPsorDUBs)removeubiquitinsfromtargetproteins(4).ThereversiblenatureofubiquitinationfurtherenhancesthepotentialofthisPTMtodynamicallyregulateproteinfunction.
References
1)Grabbe,C.etal.2011.Thespatialandtemporalorganizationofubiquitinnetworks.Nat.Rev.Mol.Cell.Biol.12:295-307.
2)DeshaiesR.J.&Joazeiro,C.A.2009.RINGdomainE3ligases.Ann.Rev.Biochem.78:399-434.
3)Lomeli,H.&Vazquez.2011.EmergingrolesoftheSUMOpathway.CellMol.LifeSci.68:4045-4064.
4)Faesen,A.C.etal.2012.TheroleofUBLdomainsinubiquitinspecificproteases.Biochem.Soc.Trans.40:539-545.
5)Zuin,A.etal.2014.Ubiquitinsignaling:Extremeconservationasasourceofdiversity.Cells3:690-701.
Formoreinformationcontact:signalseeker@Cytoskeleton.com
AssociatedProducts:
Signal-Seeker™UbiquitinationDetectionKit(Cat.#BK161)
Signal-Seeker™UbiquitinAffinityBeads(Cat.#UBA01-beads)
Signal-Seeker™:BlastR™RapidLysatePrepKit(Cat.#BLR01)
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想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
