Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)EPKVKTQWPRSADE,correspondingtoaminoacidresidues277-290ofmouseBAI2(Accession Q8CGM1).Extracellular,N-terminus.
- Westernblotanalysisofrat(lanes1and3)andmouse(lanes2and4)brainmembranes:1,2. Anti-BAI2(extracellular)Antibody(#ABR-022),(1:200).
3,4.Anti-BAI2(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.WesternblotanalysisofhumanSHSY-5Yneuroblastomacelllinelysates:1. Anti-BAI2(extracellular)Antibody(#ABR-022),(1:200).
2.Anti-BAI2(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.
- 1.Okajima,D.etal.(2009)J.Recept.SignalTransduct.Res.30,143.
- 2.Jeong,B.C.etal.(2006)FEBSLett.580,669.
Brain-specificangiogenesisinhibitor2(BAI2)isamemberoftheadhesionG-proteincoupledreceptor(GPCR)family.BAI2,andothermembersofitsfamily,commonlyhavealongN-terminalextracellularregion(ECR)containingaGPCRproteolysissite(GPS)adjacenttotheseven-transmembrane(7TM)domain.ThelongN-terminuscontainscommonstructuraldomains,includingthrombospondintype-Irepeats(TSRs),epidermalgrowthfactor(EGF)-like,leucine-richrepeats,lectin-like,immunoglobulin(Ig),andcadherins.BAI2iscomposedof521aminoacidsandismainlyexpressedinneurons.
AlthoughBAI2isaGPCR,therearecurrentlynoreportsshowingevidenceofanyphysiologicalligands.Instead, BAI2isactivatedbycleavageanddissociationoftheECRfromits7TMfragment,andfunctionsasaGPCR.BAI2isglycosylatedandcleavedattheGPSbyfurinintheER.ThecleavedN-terminalECRassociateswiththeC-terminal7TMfragmentandtheyaretranslocatedtothecellsurface.TheECRassociatedwiththe7TMfragmentinteractswithothermembraneproteinsormatrixproteinsthroughtheTSRsanddissociatesfromthe7TMfragment.The7TMfragmentwhichhasreleasedtheassociatedECRchangesitsconformationintotheactivestateandactivatestheGprotein-coupledsignaling1.
BAI2isinvolvedintheearlystagesofneovascularizationofcerebralcortexafterischemia.DecreasedlevelsofBAI2induceincreasedVEGFexpressionmediatedbyGA-bindingproteingamma(GABPγ)incerebralischemiaandundernormalconditions2.
Anti-BAI2(extracellular)Antibody(#ABR-022)isahighlyspecificantibodydirectedagainstanepitopeofthemouseprotein.Theantibodycanbeusedinwesternblotanalysis.Theantibodyrecognizesanextracellularepitope,andcanpotentiallybeusedfordetectingtheproteininlivingcells.IthasbeendesignedtorecognizeBAI2fromrat,mouse,andhumansamples.
ebiomall.com
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你看这个抗体的质量怎么样,说明里面有没有说可以做组化,还是只能做western blot。
第二个问题:兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”,如果对自己的蛋白产生免疫反应,那就麻烦了。
可以类比器官移植,亲缘关系越近,越不容易产生免疫排斥。
以下来自摆渡百科
细胞在发生凋亡时,会激活一些DNA内切酶,这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测,可以发现180-200bp的DNA ladder。基因组DNA断裂时,暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ,从而可以通过荧光显微镜或流式细胞仪进行检测,这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药,那工作人员说不是抗生素怎么消炎呢,请问知情网友求一替代品,谢了。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
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