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Antigen Incl. This antibody is shipped with its antigen FREE of charge!
- Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.
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Western blot analysis of mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes:1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:500).
3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, preincubated with the negative control antigen.
Western blot analysis of rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes:1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:200).
3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, preincubated with the negative control antigen.
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Expression of Angiotensin II Receptor Type-1 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011). A. Mouse anti-Parvalbumin (red) is detected in the Purkinje layer. B. In the same section, AT1 receptor (green) is also present in the Purkinje layer. Arrows point at AT1 receptor immunoreactive cells. Merge of A and B panels reveals partial co-localization.
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Cell surface detection of AGTR1 in live intact human Jurkat T-cell leukemia cells:___ Unstained cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (5 µg) + goat-anti-rabbit-FITC.
___ Cells + Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, (10 µg) + goat-anti-rabbit-FITC.The negative control antigen is not suitable for this application.
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Expression of AGTR1 in rat C6 glioma cellsCell surface detection of AGTR1 in live intact rat C6 glioma cells. A. Cells were stained using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Live intact C6 cells.
- 1. Sasaki, K. et al. (1991) Nature 351, 230.
- 2. Murphy, T.J. et al. (1991) Nature 351, 233.
- 3. De Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415.
- 4. Hunyady, L. and Catt, K.J. (2006) Mol. Endocrinol. 20, 953.
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Angiotensin II receptor type 1 (AT1 receptor, AGTR1) is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II).
Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects.
Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (IP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by IP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration.
In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc.
As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.
Expression of AT1R in mouse satellite cells and mouse myofibers.A. Indirect flow cytometry of mouse satellite cells using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011). B. Immunocytochemical staining of intact cultured mouse myofibers using same antibody as in A.Adapted from Yoshida, T. et al. (2013) J. Biol. Chem. 288, 23823. with permission of the American Society for Biochemistry and Molecular Biology.
Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize AT1 receptor from rat, mouse, and human samples.
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第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
