Lyophilized Powder This product is freeze dried. All water molecules have been removed.
- Peptide NKSLSSFKENEENIQC, corresponding to amino acid residues 84-99 of rat CB1 receptor (Accession P20272). Extracellular, N-terminus.
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Cell surface detection of CB1 receptor in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Cannabinoid Receptor 1 (extracellular)-FITC Antibody (#ACR-001-F), (2.5 µg).
Cell surface detection of CB1 receptor in live intact mouse P815 mastocytoma cell line:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Cannabinoid Receptor 1 (extracellular)-FITC Antibody (#ACR-001-F), (5 µg).
- 1. Howlett, A.C. (2002) Prostaglandins Other Lipid Mediat. 68, 619.
- 2. Guo, J. and Ikeda, S.R. (2004) Mol. Pharmacol. 65, 665.
- 3. Wotherspoon, G. et al. (2005) Neuroscience 135, 235.
- 4. Gong, J.P. et al. (2006) Brain Res. 1071, 10.
- 5. Fernandez-Ruiz, J. et al. (2007) Trends Pharmacol. Sci. 28, 39.
- 6. Sarfaraz, S. et al. (2005) Cancer Res. 65, 1635.
- 7. Sanchez, C. et al. (2001) Cancer Res. 61, 5784.
- 8. Alberich Jorda, M. et al. (2004) Blood 104, 526.
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Cannabinoids have been used in Eastern medicine for many years as pain relievers. Δ9-tetrahydrocannabinol (THC), the major psychoactive compound in marijuana and hashish, has been shown to interact with two specific cannabinoid receptors: cannabinoid receptor 1 (CB1 receptor) and cannabinoid receptor 2 (CB2 receptor).1 The cannabinoid receptors can be distinguished by their amino acid sequences, signaling mechanisms, and tissue distributions.1 Both receptors belong to the G-protein coupled receptor (GPCR) superfamily. CB1 was shown to modulate several Ca2+ and K+ ion channels.1,2
CB1 is primarily expressed in the central nervous system. However, expression of CB1 is also detected in the peripheral terminals, in non-neuronal peripheral tissues such as uterus, testes, spleen, as well as in cells of the immune system.2,3
CB1 is implicated in many cellular functions such as neurotransmitter release, pain relief, cancer, and obesity.4,5 Growth inhibition of tumor cells was demonstrated following mixed CB1/CB2 agonist treatment in both prostate and non-melanoma skin cancers.4,5 Through their interaction with CB1, cannabinoid compounds stimulate appetite for sweets and palatable foods in particular, making CB1 an attractive therapeutic target for the treatment of obesity and eating disorders.6
Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001) is a highly specific antibody directed against an epitope of the rat CB1 receptor. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize CB1 in human, mouse, and rat samples.
Anti-Cannabinoid Receptor 1 (extracellular)-FITC Antibody (#ACR-001-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.
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第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
