- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody to human protein tyrosine phosphatase 1 C (PTP1-C)
Purification:Protein G affinity purified
Product Type:Primary antibody
Target Protein:Human protein-tyrosine phosphatase 1 C
Immunogen:Purified recombinant protein corresponding to full-length PTP1-C
Fusion Myeloma:Sp2/0-Ag14
Specificity:This antibody recognizes single 67kDa PTP1-C in human cells that express the enzyme and the SH2(C) domain.
Species Reacitvity:Human
Host / Isotype: Mouse, IgG2b Kappa
Formulation:Lyophilized from a solution in 0.01M PBS pH7.2
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Storage:Store at -20oC.Avoid repeated freeze and thaw cycles.
Research Area:Signal transduction
Background:
The phosphorylation state of receptors and signal transduction molecules influences almost every cell activities. The phosphorylation rate is regulated by kinases which add phosphate group and by phosphatases which remove phosphate group form the amino acid residues on protein molecules. Protein tyrosine phosphatase 1 C (PTP1C) is a tyrosine phosphatase highly expressed in heomatopoietic cells. PTP1C was found to co-precipitate with B lymphocyte-specific cell surface glycoprotein CD22, after anti-immunoglobulin stimulation. Study with PTP1C deficient mice showed that the phosphatase may implicate with negative selection in B cell immunity.
Application:ELISA, Western Blot, Immunocytochemistry
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
Product Specificity | mAb anti-Human PTP1-C, PTY13 |
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Application | EIA, WB, ICC |
Size | 0.1 mg |
ebiomall.com
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你看这个抗体的质量怎么样,说明里面有没有说可以做组化,还是只能做western blot。
第二个问题:兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”,如果对自己的蛋白产生免疫反应,那就麻烦了。
可以类比器官移植,亲缘关系越近,越不容易产生免疫排斥。
以下来自摆渡百科
细胞在发生凋亡时,会激活一些DNA内切酶,这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测,可以发现180-200bp的DNA ladder。基因组DNA断裂时,暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ,从而可以通过荧光显微镜或流式细胞仪进行检测,这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
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