This kit enables users to perform cDNA synthesis directly from up to 1,000 intact cells (or 10 pg–10 ng of total RNA) with high reproducibility, even gene-body coverage, and accurate representation of GC-rich transcripts. Full-length cDNA libraries produced with this kit are compatible with both Illumina and Ion Torrent sequencing platforms. The core SMART technology employed by this kit provides full-length transcript information, enabling analysis of transcript isoforms, gene fusions, point mutations, etc. Oligo(dT) priming is used to specifically amplify mRNA from either high-quality total RNA (RIN >8) or intact cells. The inclusion of LNA technology in the SMART-Seq v4 Oligo improves the efficiency of template switching, allowing for identification of higher numbers of genes relative to other methods.
This kit enables users to perform cDNA synthesis directly from up to 1,000 intact cells (or 10 pg–10 ng of total RNA) with high reproducibility, even gene-body coverage, and accurate representation of GC-rich transcripts. Full-length cDNA libraries produced with this kit are compatible with both Illumina and Ion Torrent sequencing platforms. The core SMART technology employed by this kit provides full-length transcript information, enabling analysis of transcript isoforms, gene fusions, point mutations, etc. Oligo(dT) priming is used to specifically amplify mRNA from either high-quality total RNA (RIN >8) or intact cells. The inclusion of LNA technology in the SMART-Seq v4 Oligo improves the efficiency of template switching, allowing for identification of higher numbers of genes relative to other methods.
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing is the fourth-generation of SMARTer ultra-low kit, and our most sensitive solution for ultra-low inputs. This kit takes SMART (Switching Mechanism at 5' End of RNA Template) technology to the next level by improving upon the Smart-seq2 method and incorporating locked nucleic acid (LNA) technology in the SMART-Seq v4 Oligo. With these additions—referred to as "SMARTer-Seq® chemistry"—this kit outperforms both the SMART-seq2 method and previous generations of SMARTer kits by identifying the greatest number of transcripts.
Information about prior SMARTer Ultra Low RNA kits can be found here.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
