
The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. The system utilizes Cre recombinase, a bacteriophage P1 enzyme that recognizes a short target DNA sequence (loxP site). The AAVpro Helper Free System (AAV2-CRE Recombinase) is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) viral particles to deliver the Cre recombinase gene cassette to mammalian cells.
The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. The system utilizes Cre recombinase, a bacteriophage P1 enzyme that recognizes a short target DNA sequence (loxP site). The AAVpro Helper Free System (AAV2-CRE Recombinase) is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) viral particles to deliver the Cre recombinase gene cassette to mammalian cells. High-titer AAV2 particles can be prepared without a helper virus. HEK 293T cells are transfected with plasmids encoded in this kit, and AAVpro Extraction Solution is used for efficient AAV2 particle isolation.
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以下来自摆渡百科
细胞在发生凋亡时,会激活一些DNA内切酶,这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测,可以发现180-200bp的DNA ladder。基因组DNA断裂时,暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ,从而可以通过荧光显微镜或流式细胞仪进行检测,这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
你看这个抗体的质量怎么样,说明里面有没有说可以做组化,还是只能做western blot。
第二个问题:兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”,如果对自己的蛋白产生免疫反应,那就麻烦了。
可以类比器官移植,亲缘关系越近,越不容易产生免疫排斥。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息,你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药,那工作人员说不是抗生素怎么消炎呢,请问知情网友求一替代品,谢了。

