The LEGENDplex™ Mouse B cell Panel (13-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel focuses on 13 key biomarkers involved in B cell function, activation, and survival. It allows for simultaneous quantification of 13 mouse targets such as IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BAFF, BCMA, sCD40L, and IL-10. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.
The LEGENDplex™ Mouse B cell Panel is configured as shown below depending on sample types and required dilutions:
Catalog No. | Plex Size | Targets | Recommended Sample Type | Recommended Dilution Factor |
740818 740819 | 13-plex | IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BAFF, BCMA, sCD40L, IL-10 | Tissue Culture* | Varies |
740826 740827 | 12-plex | IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BCMA, sCD40L, IL-10 | Serum, Plasma | 2 |
Tissue Culture | Varies | |||
740982 740983 | 1-plex | BAFF | Serum, Plasma | 25 |
Tissue Culture | Varies | |||
740824 740825 | 3-plex | IL-12p70, TNF-α, IFN-γ | Serum, Plasma | 2 |
Tissue Culture | Varies | |||
740822 740823 | 5-plex | IL-4, IL-6, IL-2, TNF-α, IL-13 | Serum, Plasma | 2 |
Tissue Culture | Varies | |||
740820 740821 | 8-plex | IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, IL-13, IFN-γ | Serum, Plasma | 2 |
Tissue Culture | Varies | |||
740828 740829 | 2-plex | Free Active TGF-β1, IL-10 | Serum, Plasma | 2 |
Tissue Culture | Varies |
*Note: Serum and plasma are not the recommended sample type. Refer to Sample Dilution section in the manual for details.
The LEGENDplex™ Mouse B cell Panel is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel. This assay is for research use only.
Product Detailsebiomall.com
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以下来自摆渡百科
细胞在发生凋亡时,会激活一些DNA内切酶,这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测,可以发现180-200bp的DNA ladder。基因组DNA断裂时,暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ,从而可以通过荧光显微镜或流式细胞仪进行检测,这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
第二个问题:兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”,如果对自己的蛋白产生免疫反应,那就麻烦了。
可以类比器官移植,亲缘关系越近,越不容易产生免疫排斥。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息,你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药,那工作人员说不是抗生素怎么消炎呢,请问知情网友求一替代品,谢了。
你看这个抗体的质量怎么样,说明里面有没有说可以做组化,还是只能做western blot。