The JES6-5H4 antibody binds to mouse Interleukin-2 (IL-2), a 17 kDa cytokine that is secreted by activated T cells. This cytokine acts as an autocrine stimulatory factor for T cell proliferation, differentiation and survival, as well as being an effective activator of B cells and inducer of NK cell cytotoxic functions. IL-2 plays a key modulatory role in the differentiation of T cells toward either Th17 or T regulatory (Treg) cell types and is important for Treg cell function. The IL-2 receptor (IL-2R) is a complex consisting of an IL-2R alpha chain (CD25), along with the IL-2R beta chain (CD122) and the common gamma chain (CD132). IL-2 may also bind with low affinity to the T cell surface protein CD25 alone, although this interaction does not induce cell signaling.
The JES6-5H4 antibody is extensively used for intracellular detection of IL-2 by flow cytometry, for detection of soluble cytokine in ELISA, and as a neutralizing reagent in vitro and in vivo.
Attridge K, Wang CJ, Wardzinski L, Kenefeck R, Chamberlain JL, Manzotti C, Kopf M, and Walker LSK. 2012. Blood. 119: 4656-4664. (Flow cytometry)
Helbig C, Gentek R, Backer RA, de Souza Y, Derks IAM, Eldering E, Wagner K, Jankovic D, Gridley T, Moerland PD, Flavell RA, and Amsen D. 2012. Proc. Natl. Acad. Sci. 109: 9041-9046. (ELISA - detection)
Chen Q, Kiim YC, Laurence A, Punkosdy GA, and Shevach EM. 2011. J. Immunol. 186: 6329-6337. (in vivo blocking)
Weber KS, Hildner K, Murphy KM and Allen PM. 2010 J. Immunol. 185: 2836-2846. (ELISA)
Wang C, Morley SC, Donermeyer D, Peng I, Lee WP, Devoss J, Damilenko DM, Lin Z, Zhang J, Zhou J, Allen PM, and Brown EJ. 2010. J. Immunol. 185: 7487-7497. (Immunocytochemistry/Immunofluorescence microscopy)
Cardona AE and Teale JM. 2002. J. Immunol. 169: 3163-3171. (Immunohistochemistry - frozen tissue)
Woo AL, Gildea LA, Tack LM, Miller ML, Spicer A, Millhorn DE, Finkelman FD, Dassett DJ, and Shull GE. 2002. J. Biol. Chem. 277: 49036-49046. (in vivo blocking)
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想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
