The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.
The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will "compete" for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.
Taniguchi RT, DeVoss JJ, Moon JJ, Sidney J, Sette A, Jenkins MK, and Anderson MS. 2012. Proc. Natl. Acad. Sci. 109: 7847-7852. (in vivo depletion)
Wolkers MC, Gerlach, C, Arens R, Janssen EM, Fitzgerald P, Schumacher TN, Medema JP, Green DR, and Schoenberger SP. 2012. Blood. 119:798-804. (in vivo depletion)
Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).
Thornton EE, Looney MR, Bose O, Sen D, Sheppard D, Locksley R, Huang X, and Krummel MF. 2012. J. Exp. Med. 10:1084. (Immunohistochemistry - OCT embedded frozen tissue)
Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Immunoprecipitation)
Hammerbeck CD and Hooper JW. 2011. J. Virol. 85(19) 9929-9944. (Flow cytometry - Syrian hamster).
Diamond MS, Kinder M, Matsushita H, Mashayekhi M, Dunn GP, Archambault JM, Lee H, Arthur CD, White JM, Kalinke U, Murphy KM, and Schreiber RD. 2011. J. Exp. Med. 208: 1989-2003. (in vitro blocking)
Kao H, Lin J, Littman DR, Shaw AS, and Allen PM. 2008. J. Immunol. 181: 8248-8257. (Immunoprecipitation)
Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross M, suri A, and Allen PM. 2007. Nat. Immunol. 8:388-397. (in vitro blocking)
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第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
