The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.
The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.
Panchal RG, Ulrich RL, Bradfute SB, Lane D, Ruthel G, Kenny TA, Iversen PL, Anderson AO, Gussio R, Raschke WC, and Bavari S. 2009. 284: 12874-12885. (Immunoprecipitation)
Cherpes TL, Busch JL, Sheridan BS, Harvey SAK, and Hendricks RL. 2008. J. Immunol. 181: 969-975. (Complement-mediated cell depletion)
Cheng G, Zhang H, Yang X, Tzima E, Ewalt KL, Schimmel P, and Faber JE. 2008. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295: R1138-R1146. (Immunohistochemistry - paraffin embedded tissue)
Nguyen JT, Evans DP, Galvan M, Pace KE, Leitenberg D, Bui TN, and Baum LG. 2001. J. Immunol. 167: 5697-5707. (Immunofluorescence microscopy, Immunoprecipitation)
Czyzyk J, Leitenberg D, Taylor T, and Bottomly K. 2000. Mol. Cell. Biol. 20(23): 5740-5747. (Western Blot)
Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273: 30680-30687. (Western Blot)
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
