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GenWay/Peroxidase Conjugated Affinity Purified Anti-CHICKEN IgG (H&L) (RABBIT)/GWB-35B4C7/1.5 mg
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4000-520-616
Description:
Antigen:IgGFraction:AffinityPurifiedIgG
AdditionalInformation:
Name | PeroxidaseConjugatedAffinityPurifiedAnti-CHICKENIgG(H&L)(RABBIT) |
---|---|
RelatedProductNames | PeroxidaseConjugatedAffinityPurifiedanti-ChickenIgG[H&L][Rabbit];N/APeroxidaseConjugatedAffinityPurifiedAnti-CHICKENIgG(H&L)(RABBIT)IGHG1 |
SpeciesReactivity | Chicken |
Host | Rabbit |
Isotype | IgG |
Conjugation | Peroxidase(HorserADIsh)-HRP |
Immunogen | ChickenIgGwholemolecule |
ProductFormat | Lyophilized |
SwissProtNumber | P01857 |
Purification | Anti-ChickenIgG/IgYSecondaryAntibodywaspreparedfrommonospecificantiserumbyimmunoaffinitychromatographyusingChickenIgGcoupledtoagarosebeadsfollowedbysolidphaseadsorption(s)toremoveanyunwantedreactivities. |
Specificity | Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Peroxidase,anti-RabbitSerumandChickenIgG.Somelightchaincrossreactivitymaybeobservedagainstchickenimmunoglobulins.KnownCrossReactivity: ChickenIgY/IgG |
Applications | WB,ELISA,IHC |
ApplicationInfo | Allassaysshouldbeoptimizedbytheuser.Recommendeddilutionsmaybelistedbelow. ELISA:1:75,000-1:150,000 WB:1:2,500-1:15,000 IHC:1:1,000-1:5,000 |
Format | Lyophilized |
ReconstitutionandStorage | Storevialat4Cpriortorestoration.Restorewith1.0mLofdeionizedwater(orequivalent).Forextendedstoragealiquotcontentsandfreezeat-20Corbelow.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4Casanundilutedliquid.Diluteonlypriortoimmediateuse.Expirationdateisone(1)yearfromdateofrestoration. |
Reactivity | ChickenIgG |
Storage | 4C |
LeadTime | Domestic:within1-2weeksdelivery International:1-2weeks |
IntendedUse | ResearchUseOnly |
AdditionalInformation | Buffer:0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Color:Substratedependent ConjugationReference:ModifiedfromFarr&Nakane,J.Immunol.Methods47;129-144.1981. |
:: | Preservative:0.01%(w/v)GentamicinSulfate(DoNOTaddSodiumAzide!) StABIlizer:10mg/mlBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree |