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GenWay/Rat IgG2b Heavy Chain Antibody/GWB-7A9F7E/0.5 mg
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4000-520-616
Description:
ShortDescription:MOUSEANTIRATIgG2bHEAVYCHAINAdditionalInformation:
Name | RatIgG2bHeavyChainAntibody |
---|---|
RelatedProductNames | MOUSEANTIRATIgG2bHEAVYCHAINIgG2bIgh-3 |
GeneSymbol | Igh-1a |
Geneid | 299352 |
AliasSymbols | Igh-1a |
ProteinName | Iggamma-2BchainCregion |
DescriptionofTarget | MOUSEANTIRATIgG2bHEAVYCHAIN |
SwissprotID | P20761 |
SpeciesReactivity | Rat |
Clone | MARG2b-3 |
Host | Mouse |
Isotype | IgG1 |
Immunogen | RatIgG2b |
Homology | Rat |
ProductFormat | PurifiedIgG-liquid |
Specificity | IgG2b |
Concentration | 1mg/ml |
Applications | E |
ApplicationInfo | ELISA:ThisantibodymaybeusedasacoatingantibodyinasandwichELISAincombinationwithcloneMARK-1/MARL-15(GWB-9BA2B896P)asdetectionreagentandpurifiedratIgG2b(GWB-BB9356). |
Clonality | Monoclonal |
Format | Solution:Phosphatebufferedsaline,Stabalizer:0.1%,SodiumAzide,Form:PurifiedIgG-liquid |
StABIlity | 18monthsfromdateofdespatch. |
ReconstitutionandStorage | Storeat+4oCorat-20oCifpreferred. Thisproductshouldbestoredundiluted. Storageinfrostfreefreezersisnotrecommended.Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.Shouldthisproductcontainaprecipitatewerecommendmicrocentrifugationbeforeuse. |
Reactivity | Rat |
Storage | Storeat+4°Corat-20°Cifpreferred.Thisproductshouldbestoredundiluted.Storageinfrostfreefreezersisnotrecommended.Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.Shouldthisproductcontainaprecipitate |
ProtocolInformation | Citation:1:YangCP,ShittuE,BellEB.SpecificBcelltoleranceisinducedbycyclosporinAplusdonor-specificbloodtransfusionpretreatment:prolongedsurvivalofMHCclassIdisparatecardiacallografts.JImmunol.2000Mar1;164(5):2427-32.PubMedPMID:10679079. Species:Rat ExperimentName:1.Cellseparation2.Alloantibodydeterminationbyflowcytometry3.CytotoxicAbdetermination ExperimentBackground:1.UsingapublishedprotocolforprolongingthesurvivalofkidneyallograftsinthehighresponderRT1ustrainofrat,Chun-PingYang,EmmaShittu,andEricB.Belldeterminedwhetherthesamepreoperativetreatmentwaseffectiveinenhancingsurvivalofcardiacallografts.2.AnexaminationofAbproductioninCD4Tcell-injectednuderatsindicatedthattheonsetofrejectioninthecontrolgroupcorrespondedwithproductionofcytotoxicAb.3.Anti-MHCclassIAbproductioninR8heart-grafted,CD4Tcell-reconstitutedRT1unuderats ExperimentalSteps:1.CellseparationThoracicductlymphocytesweredepletedofBcellsandCD8Tcellsusingamixtureofmouseanti-ratmAbsgrowninhouseasascitesorpurchasedfrom(Kidlington,Oxford,U.K.):OX12(anti-Igk),OX6(anti-MHCclassII),andOX8(anti-CD8).Stainedlymphocyteswereremovedbytwoorthreeroundsofmagneticadherenceusinganti-mouseIg-conjugatedimmunomagneticparticles.Theresultingpopulationofcellswas95–98%CD41.Bcellswereobtainedfromthoracicductlymphofathymicnuderats;80–85%ofcellswereIg+andvoidoffunctioningTcells.2.AlloantibodydeterminationbyflowcytometrySerafromRT1uanimalswereseriallydilutedandmixedwithRBCfromR8ratstodetectanti-MHCclassI(Aa)alloantibody.Afterincubation,theredcellswerewashedandstainedwithFITC-sheepanti-ratIgGorwithmousemAbagainstratIgG1(MARG1-2),IgG2a(MARG2a-1),andIgG2b(MARG2b-3),followedbyFITC-F(ab’)2anti-mouseIg(Dako,HighWycombe,U.K.)absorbedagainstsolidphaseratIg.Thetiterforeachsamplewasdeterminedasthereciprocaldilution(log3)atwhichthepercentageofpositivelystainedcellswas.>5%abovethatofanormalratserumcontrol.3.CytotoxicAbdeterminationTheprocedurewasadaptedfromthatofBradleyetal..Briefly,50µlofseriallydilutedtestsera,induplicate,wasaddedto96-wellroundbottommicrotiterplates(AlphaLaboratories,Eastleigh,U.K.),and50µlof51Cr-labeledConA-stimulatedblastcellsat106permlinRPMI1640plus10mMHEPESwith5%FCSwereaddedtoeachwell.Plateswereincubatedfor30minat37°C,100mlof1/25dilutedbabyrabbitcomplementwasadded,andtheplateswereincubatedfor1hat37°C.Maximumreleasewasobtainedbytheadditionof100mlHClinplaceofcomplement.Theplateswerecentrifuged,and100-mlaliquotsofsupernatantcontainingreleased51Crwereremovedandcounted.Specificreleasewascalculatedbytheformula:100x[(experimentalrelease-spontaneousrelease)/(maximumrelease-spontaneousrelease)]. NumberOfProtocols:3 |
LeadTime | Domestic:within2-3weeksdelivery International:2-3weeks |
IntendedUse | ResearchUseOnly |
KeyReference | 1.LeRoy,D.etal.(1999)Monoclonalantibodiestomurinelipopolysaccharide(LPS)-bindingprotein(LBP)protectmicefromlethalendotoxemiabyblockingeitherthebindingofLPStoLBPorthepresentationofLPS/LBPcomplexestoCD14.J.Immunol.162:7454-7460. |
:: | Preparation:PurifiedIgGpreparedbyaffinitychromatography PreservativeStabilisers:0.09%-SodiumAzide |
:: | ApproxProteinConc:IgGconcentration1mg/ml BufferSolutions:Phosphatebufferedsaline |