
Overview:
c-MET is a receptor tyrosine kinase that binds hepatocyte growth factor (also known as scatter factor, HGF/SF). Stimulation by HGF causes c-MET to autophosphorylate at multiple tyrosines, including Y1234 and Y1235. Activated c-MET binds to various substrates, such as GAB1, GRB2, phosphatidylinositol 3-kinase (PI3K) and others. GAB1 is scaffold protein, which enables c-MET to elicit downstream signaling cascades, which include the well-characterized ERK/MAPK, PI3K–Akt/PKB, Crk–Rap and Rac–Pak pathways; leading to cell proliferation and cell survival.
References:
1. Birchmeier, C., et al.: Met, metastasis, motility and more. Nat. Rev. Mol. Cell Bio. 2003: 4; 915-925.2. Fan, S., et al.: The multisubstrate adapter Gab1 regulates hepatocyte growth factor (scatter factor)-c-Met signaling for cell survival and DNA repair. Mol. Cell Biol. 2001: 21; 4968-84.3. Longati, P., et al. Receptor tyrosine kinases as therapeutic targets: the model of the MET oncogene. Curr. Drug Targets. 2001: 2; 41-55.
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因此所有羊抗兔可以结合兔子的同种异型抗体,也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属:兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应:鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用:用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验

