
Overview:
BID is a BH3 interacting death domain that heterodimerizes with either agonist BAX or antagonist BCL2 (1). BID is a member of the BCL-2 family of cell death regulators and is a mediator of mitochondrial damage induced by caspase-8 (CASP8). BID initiates apoptosis by binding to regulatory sites on prosurvival BCL2 proteins to directly neutralize their function. Multiple alternatively spliced transcript variants of BID have been found, but the full-length nature of some variants has not been defined. BID together with Cathepsins play an important role in the actions of Camptothecin on breast cancer cells (2).
References:
1. Hayakawa, A. et al:, Bid truncation mediated by caspases-3 and -9 in vinorelbine-induced apoptosis. Apoptosis. 2008;13(4):523-30.2. Lamparska-Przybysz. M. et al: Cathepsins and BID are involved in the molecular switch between apoptosis and autophagy in breast cancer MCF-7 cells exposed to camptothecin. J PhysiolPharmacol. 2005 Jun;56Suppl 3:159-79.
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因此所有羊抗兔可以结合兔子的同种异型抗体,也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属:兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应:鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用:用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验

