
Overview:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Tyrosine Hydroxylase antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).
References:
1. Dunkley PR, Bobrovskaya L, et al. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. 2001.2. Salvatore MF, Waymire JC, Haycock JW, Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360. 2001.3. Witkovsky P, Gabriel R, Haycock JW, Meller E, Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116. 2000.4. Xu ZQ, Lew JY, Harada K, et al, Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies. Neurosci 82:727-738. 1998.5. Zhu MY, Klimek V, Haycock JW, Ordway GA, Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. 2000.6. Zhu MY, Klimek V, Dilley GE, et al, Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286. 1999.
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因此所有羊抗兔可以结合兔子的同种异型抗体,也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属:兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应:鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用:用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验

