Lyophilized Powder This product is freeze dried. All water molecules have been removed.
- Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.
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Cell surface detection of AT1 receptor in live intact human THP-1 monocytic leukemia cells:___ Cells alone.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody (#AAR-011-F), (5 µg).
Cell surface detection of AT1 receptor in live intact human Jurkat T-cell leukemia cells:___ Cells alone.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody (#AAR-011-F), (5 µg).
- 1. de Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415.
- 2. Balakumar, P. and Jagadeesh, G. (2014) J. Mol. Endocrinol. 53, R71.
- 3. Graus-Nunes, F. et al. (2017) Mol. Cell. Endocrinol. 439, 54.
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The biological functions of angiotensin II are mediated by AT1 and AT2 receptors. Both belong to the G-protein coupled receptor superfamily and are comprised of seven hydrophobic transmembrane segments forming α helices in the lipid bilayer of the cell membrane, an extracellular N-terminus and an intracellular N-terminus1.
Two highly homologous isoforms of the receptor, AT1A and AT1B have been identified in rodents. The AT1A receptor accounts for 90% of the total binding, and is predominant in the kidney, vascular smooth muscle cells, heart, liver, and in some areas of the brain, while the AT1B receptor is found predominantly in the pituitary and adrenal glands, placenta, lung, and brain. These isoforms are pharmacologically indistinguishable and are both selectively antagonized by losartan.
Through AT1 receptor, angiotensin II stimulates multiple signaling pathways, cross-talks with several tyrosine kinases, and trans-activates growth factor receptors. Phosphorylation of the receptor by GPCR kinases terminates receptor activation and promotes β-arrestin recruitment. β-arrestin-scaffolded signaling mediates ‘secondary signaling’ that involves multiple kinases that link to cell protective downstream signaling molecules2.
In a model of diet-induced obese mice, AT1 receptor inhibition demonstrates beneficial pleiotropic effects. Mice receiving the treatment exhibit enhanced pancreatic duodenal homeobox 1 (PDX1) and GLP-1 pancreatic islet expression. PDX1 islets are essential for the expression of glucokinase and GLUT2. Greater GLP-1 levels, islet vascularization and reduced apoptosis and macrophage infiltration are also observed3.
Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize AT1 receptor from rat, mouse, and human samples.
Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody (#AAR-011-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.
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因此所有羊抗兔可以结合兔子的同种异型抗体，也就是说只要是兔子产生的抗体，羊抗兔（也叫抗抗体或者2抗）都可与之结合。
在试验中，二抗经常被做了手脚，打上了标记，比如辣根过氧化物酶，经常被用在ELISA，WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属：兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记：HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应：鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用：用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验
