
PRODUCT SPECIFICATION
Product# 1102: Murine Anti-tat HIV-1 IIIB Monoclonal Antibody
SPECIFICATIONS APPLICATIONS
Antibody Class: IgG1 ELISA, Western ELISA
Concentration: See vial Immunoprecipitation
Mass/vial: 100µg Immunocytochemistry
Purity: >95% Virus Neutralization in culture
Stabilizer: None
Preservative: None
Physical State: Frozen Liquid
Storage: -75oC
Stability: At least 12 months at -75oC
DESCRIPTION: High affinity murine anti-tat HIV-1.
SPECIFICITY: Binds to native and recombinant HIV-1 tat in ELISA and Western ELISA. Epitope maaped to N-terminus of HIV-1 tat.
BIOLOGICAL
ACTIVITY: Neutralizes tat dependent HIV-1 replication in infected monocytic cells.
APPLICATIONS AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml.
RELATED PRODUCTS
PRODUCT#: 1102-P Peroxidase-Conjugated Murine anti-tat HIV-1 IIIB
Application: ELISA, Western ELISA, Histocytochemistry.
PRODUCT#: 1102-F Fluorescene Murine anti-tat HIV-1 IIIB
Application: FACS, Cell Staining
PRODUCT#: 1102-G Colloidal Gold-Conjugated Murine anti-tat HIV-1 IIIB
Application: Dot Blots, EM.
PRODUCT#: 1102-M Magnetic Murine anti-tat HIV-1 IIIB
Application: Immunoprecipitation.
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因此所有羊抗兔可以结合兔子的同种异型抗体,也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属:兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应:鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用:用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验

