

Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
- PeptideCMIEWPEHPNRTYEK,correspondingtoaminoacidresidues180-194ofratNK1 receptor(Accession P14600).2ndextracellularloop.
- CellsurfacedetectionofNK1RinliveintactmouseJ774macrophagecells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+ Anti-Neurokinin1Receptor(NK1R) (extracellular)-FITCAntibody(#ATR-001-F),(2.5µg).CellsurfacedetectionofNK1RinliveintacthumanTHP-1monocyticleukemiacells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+ Anti-Neurokinin1Receptor(NK1R)(extracellular)-FITCAntibody(#ATR-001-F),(2.5µg).
- 1.Pennefather,J.N. etal. (2004) LifeSci. 74, 1445.
- 2.Hershey,A.D. etal. (1991) J.Biol.Chem. 266, 4366.
- 3.Gerard,N.P. etal. (1993) Regul.Pept. 43, 21.
- 4.Caberlotto,L. etal. (2003) Eur.J.Neurosci. 17, 1736.
- 5.Munoz,M. etal. (2011) ExpertOpin.Ther.Targets 15, 889.
- 6.Ebner,K. etal. (2009) Curr.Pharm.Des. 15, 1647.
- 7.May,A.andGoadsby,P.J. (2001) ExpertOpin.Investig.Drugs 10, 673.
- 8.Palecek,J. etal. (2003) Neuroscience 116, 565.
SubstanceP(SP),NeurokininA(NKA)andNeurokininB(NKB)areallpeptidesbelongingtotheTachykininproteinfamily.Thesethreepeptideswhichdemonstrateaquiteheterogeneityintheirdistributionexerttheireffectviathreereceptors:Neurokinin1-3receptors,membersoftheG-proteincoupledreceptorsuperfamily.However,Neurokinin1Receptor(NK1)preferentiallybindsSubstanceP,Neurokinin2Receptor(NK2)toNKAandNeurokinin3Receptor(NK3)toNKB1.
Neurokininreceptorsaredistinguishedbytheirseventransmembranedomains,anextracellularN-terminusandacytosolicC-terminal.Anunusualpropertyofthesereceptorsisthepresenceofintronsaspartoftheirstructuralorganization1-3.Tachykininreceptorsundergoalternativesplicing.Forexample,NK1 isdetectedwithdifferentC-terminallengths.Thelongerreceptorisoformisfoundinthebrainwhereasthetruncatedformismostlydetectedintheperiphery1,4.
Duetothebroadexpressionprofileoftachykininpeptides,theirrespectivereceptorsarealsoexpressedinasimilarfashion.NK1 iswidelyexpressedinneuronsendothelialcells,muscleandimmunesystemcells.NK2 isbroadlyexpressedintheperipheryanditsexpressioninthebrainisquiterestricted.NK3 ontheotherhandislargelyexpressedinthecentralnervoussystemandisalsodetectedintheuterus,skeletalmuscle,lungandliver1.
Neurokininreceptorshavebeenfoundinmanypathophysiologicalindicationsandhavethereforebecometargetsforthedevelopmentofpharmacologicalcompounds.Suchindicationsincludecancer,psychologicaldisorders,migraineandvariousinflammations,justtonameafew5-8.
Anti-Neurokinin1Receptor(NK1R)(extracellular)Antibody (#ATR-001)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,indirectlivecellflowcytometry,andlivecellimagingapplications.IthasbeendesignedtorecognizeNK1 fromrat,mouse,andhumansamples.
Anti-Neurokinin1Receptor(NK1R)(extracellular)-FITCAntibody (#ATR-001-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.
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因此所有羊抗兔可以结合兔子的同种异型抗体,也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属:兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应:鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用:用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验

