GHisamemberofthesomatotropin/prolactinfamilyofhormoneswhichplayanimportantroleingrowthcontrol.Thegene,alongwithfourotherrelatedgenes,islocatedatthegrowthhormonelocusonchromosome17wheretheyareinterspersedinthesametranscriptionalorientation;anarrangementwhichisthoughttohaveevolvedbyaseriesofgeneduplications.Thefivegenessharearemarkablyhighdegreeofsequenceidentity.Alternativesplicinggeneratesadditionalisoformsofeachofthefivegrowthhormones,leADIngtofurtherdiversityandpotentialforspecialization.Thisparticularfamilymemberisexpressedinthepituitarybutnotinplacentaltissueasisthecasefortheotherfourgenesinthegrowthhormonelocus.Mutationsinordeletionsofthegeneleadtogrowthhormonedeficiencyandshortstature.
GHR,GHBP,GHreceptor,Somatotropinreceptor.
EscherichiaColi.
SterileFilteredWhitelyophilized(freeze-dried)powder.
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.3%NaHCO3pH-8.
Greaterthan98.0%asdeterminedby:(a)AnalysisbySEC-HPLC.(b)AnalysisbySDS-PAGE.
Lyophilizedformstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-20oC.
Uponreconstitution,shouldbestoredat4oCbetween2-7daysandforfutureusebelow-20oC.