ELISA Kit for Erythropoietin (EPO)
EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
- Product No.SEA028Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range6.25-400pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.68pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 349 For more details, please contact local distributors!US$ 498 For more details, please contact local distributors!US$ 2241 For more details, please contact local distributors!US$ 4233 For more details, please contact local distributors!US$ 34860 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Erythropoietin (EPO)
This assay has high sensitivity and excellent specificity for detection of Erythropoietin (EPO).No significant cross-reactivity or interference between Erythropoietin (EPO) and analogues was observed.
Recovery of the ELISA Kit for Erythropoietin (EPO)
Matrices listed below were spiked with certain level of recombinant Erythropoietin (EPO) and the recovery rates were calculated by comparing the measured value to the expected amount of Erythropoietin (EPO) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-102 | 89 |
| EDTA plasma(n=5) | 92-101 | 95 |
| heparin plasma(n=5) | 88-105 | 95 |
Precision of the ELISA Kit for Erythropoietin (EPO)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythropoietin (EPO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythropoietin (EPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Erythropoietin (EPO)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Erythropoietin (EPO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-97% | 91-99% | 96-105% | 78-94% |
| EDTA plasma(n=5) | 82-102% | 92-99% | 82-91% | 86-97% |
| heparin plasma(n=5) | 99-105% | 85-97% | 81-101% | 86-94% |
Stability of the ELISA Kit for Erythropoietin (EPO)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Erythropoietin (EPO)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Erythropoietin (EPO)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythropoietin (EPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Erythropoietin (EPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythropoietin (EPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Erythropoietin (EPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA028Mu03 | Recombinant Erythropoietin (EPO) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA028Mu01 | Recombinant Erythropoietin (EPO) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA028Mu02 | Recombinant Erythropoietin (EPO) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA028Mu02 | Polyclonal Antibody to Erythropoietin (EPO) | WB; IHC; ICC; IP. |
| PAA028Mu01 | Polyclonal Antibody to Erythropoietin (EPO) | WB; IHC; ICC; IP. |
| SEA028Mu | ELISA Kit for Erythropoietin (EPO) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA028Mu | Mini Samples ELISA Kit for Erythropoietin (EPO) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA028Mu | CLIA Kit for Erythropoietin (EPO) | Chemiluminescent immunoassay for Antigen Detection. |
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促进生长
预防疾病
如果使用抗生素，到出栏会有残留
无抗饲料可以使用宝积饲料
全程无抗
促进生长
大量使用抗生素引起药物残留。实用药物残留的动物源食品能够导致肠道菌群失调，致病菌大量繁殖，给人类健康带来危害，抗生素的残留及耐药性对公共卫生安全的威胁也愈演愈烈，在食品安全日益突出的今天，消费者对鸡蛋的安全问题更加重视。对于无抗动物产品、优质鸡蛋的需求量逐渐增加。生产绿色、安全、无抗蛋品已成为社会的主要要求，也是蛋鸡业持续发展的必然选择。
基本原则
健康的鸡不会(必)用抗生素，不用抗生素能否养出健康的鸡?
蛋鸡品种差异较大、营养不均衡、环境应激、疾病等导致蛋壳破损严重，鸡舍卫生条件差及一些肠道疾病通常会导致粪污污染加剧，鸡蛋易被微生物污染。如不使用抗生素会导致鸡只大量死亡，在实际生产过程中养殖户会不间断地使用抗生素进行蛋鸡保健和治疗。
无抗必须改变和升级
最小限度疾病(MD)种鸡群，硬件上的温湿环境有害气体与生物的全面控制，基于群体的防疫体系与健康疾病管理，基于科学的程序化的养鸡生产。在养殖管理方面，鸡群温度控制在13～24℃，空气湿度55%～65%，并有新鲜清洁的空气，光照强度维持在10～20 LX，避免低光照强度、缩短光照时间及任意更改光照时间，充分发挥蛋鸡的生产潜能；日常管理工作要保持鸡舍人员的相对稳定，定期检测鸡群的体重，观察过肥、过瘦鸡群的比例，及时调整饲喂计划，及时淘汰；避免陌生人或动物闯入鸡舍，减少停水、停电、称重、高温等因素引起的应激，避免管理不当导致蛋鸡产蛋期过早结束，提高养殖场的经济效益。
无抗生素养殖
绝对不是用某种或几种物质替代掉抗生素，而必须进行系统的思考，对养殖的软硬件体系进行设计与升级。营养方面，配方要稳定，适宜日粮营养浓度，选取原料时充分考虑霉菌毒素的影响，在蛋鸡日粮中通过添加维生素（VA、VD3、VE）、微量元素（有机铁、有机锌）、微生态制剂（寡聚糖、益生菌、酵母培养物）、复合酶制剂、植物提取物及植物精油（茶多酚、苜草素、异黄酮、桑叶粉、银杏叶提取物）来调控蛋鸡对营养物质的吸收和利用，从而提高蛋鸡的生产性能。无抗养殖要摒弃传统养殖方法，寻找新的养殖思路。驰骋祥无抗保健专家生产的“养殖安”能够很好的解决蛋鸡在无抗生产中所需的营养浓度，产品能量高，耗料少；抗应激强，死淘少。
市 场
鸡蛋营养均衡，现代膳食中的蛋白质和维生素主要来源于鸡蛋，无抗鸡蛋是老人、孕妇、小孩的首选，市场需求较大；无抗鸡蛋拥有广阔的前景，经过有机认证的无抗鸡蛋价格是传统鸡蛋价格的2～2.5倍。
禽流感新常态下，如何防控；驰骋种禽疫病防控专家魏老师建议使用药物预防禽流感：
①蛋鸡、种鸡：每间隔30天使用“驰骋芪贞颗粒或双黄连粉”+驰骋EAD乳液+养殖安混合使用；一个疗程（3-4天）
②白羽肉鸡：每间隔10天使用“驰骋芪贞颗粒或双黄连粉”+驰骋EAD乳液+养殖安混合使用。一次使用4天。抗菌药物最佳选择“好得快或替嘉康”，可以配合使用达到预防或治疗气囊炎的发生。
③种鸭鹅、种鹅、肉鸭鹅、肉鹅：每月间隔20天使用“驰骋芪贞颗粒或双黄连粉 +驰骋EAD乳液+养殖安混合使用；一个疗程（4-5天）
催肥效果很不错的
