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抗甲状腺过氧化物酶抗体检测结果_有问必答_
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productinformation
BackgroundRabbitanti-chickenIgG/Y(H&L)isasecondaryantibodyconjugatedtoHRP,whichbindstoinimmunologicalassays.AntibodyisaffinitypurifiedusingsolidphasechickenIgG/Y.
ImmunogenPurifiedChickenIgG/Y(H&L)
HostRabbit
ClonalityPolyclonal
Clone
PurityAffinitypurifiedRabbitIgG
Format
Quantity10 µl(0.91mg/ml)
Reconstitution
StorageStorelyophilizedmaterialat2-8°C.Forstorageat-20°Cafterreconstitutiondiluteantibodysolutionwithanequalvolumeofglyceroltoobtainfinalglycerolconcentrationof50%topreventlossofenzymaticactivity.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.Rehydratewith1.1mlofdeionizedwater.Preparefreshworkingdilutiondaily.
Testedapplications
RelatedproductsothersecondaryantibodiesagainstchickenIgG/Y(H&L)
Additionalinformation

Purityofthispreparationis>95%basedonSDS-PAGE.Antibodyconcentrationis1.0mg/ml.Antibodyissuppliedin10mMsodiumphosphate,0.15Msodiumchloride,pH7.2.1%(w/v)B,Protease/IgGfree.Contains0.1%(v/v)KathonCGaspreservativeofbacterialgrowth.

Basedonimmunoelectrophoresis,thisantibodyreactswith:heavychainsonchickenIgG(IgY),lightchainsonallchickenimmunoglobulins.Basedonimmunoelectrophoresis,noreactivityisobservedto:non-immunoglobulinchickenserumproteins

applicationinformation
RecommendeddilutionThisconjugateissuitableforallimmunoassayapplications.Theoptimalworkingdilutionshouldbedeterminedbytheinvestigator.Suggestedstartingdilution(s):1:500-1:5000forImmunohistochemistry1:200-1:5000forELISA/Westernblot
Expected|apparentMW
Confirmedreactivity
Predictedreactivity
Notreactivein
Additionalinformation
Selectedreferences


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