TheBM8.1antibodyisspecificformouseF4/80antigen,a125kDatransmembraneproteinwidelyexpressedbymembersofthemononuclearphagocytesystemandconsideredtobeakeyMarkerformaturemacrophagecells.F4/80isdifferentiallyexpressedduringmyeloidcelldevelopment,andmayberegulatedbycertaincytokineswithinthetissuemicroenvironment.OthercelltypesshowntoexpressthisantigenincludeLangerhanscells,Kupffercellsanddendriticcellsubsets.BM8.1iswidelyusedtogetherwithantibodiestoCD115(c-fms),CD11bandCD11ctoidentifymyeloid/macrophagecellsbyflowcytometry.
RecentPublications:
WuJandWaxmanDJ.2015.OncoImmunol.DOI:10.1080/2162402X.2015.1005521.(FlowCytometry)
| Name | APCAnti-MouseF4/80Antigen(BM8.1) |
|---|---|
| Cat.No. | 20-4801 |
| AlternativeNames | EMR1,Ly71 |
| GeneID | 13733 |
| Clone | BM8.1 |
| Isotype | RatIgG2b,κ |
| Reactivity | Mouse |
| CrossReactivity | |
| Format | APC |
| Applications | FlowCytometry |
| Citations* | IoannouM,AlissafiT,BoonL,BoumpasD,andVerginisP.2013.J.Immunol.190:2631-2640.(FlowCytometry) PapadopoulosG,WeinbergEO,MassariP,GibsonFC,WetzlerLM,MorganEF,andGencoCA.2013.J.Immunol.190:1148-1157. ChenQandSnapperCM.2013.J.Immunol.190:1048-1055. RankinAL,MummJB,MurphyE,TurnerS,YuN,McClanahanTK,BournePA,PierceRH,KasteleinRandPflanzS.2010.J.Immunol.184(3):1526-1535.(Immunohistochemistry-paraffin-embeddedtissue) GeutskensSB,OtonkoskiT,PulkkinenMA,DrexhageHAandLeenenPJ.2005.J.Leukoc.Biol.78(4):845-52(Immunohistochemistry-frozentissue) |
