
Overview:
Vimentin is a member of intermediate filament family of proteins and is an important structural protein in eukaryotic cells (1). Vimentin is a component of the cytoskeleton long with microtubules and actin microfilaments. Studies have shown that Vimentin is associated with the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol (2). Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Copolymers are frequently formed between Vimentin and other intermediate filaments, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Thus, antibodies to Vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that Vimentin plays a part in prostate cancer cell motility and invasiveness (3).
References:
1. Fuchs, E., et al: Intermediate filaments: structure, dynamics, function, and disease". Annu Rev Biochem,1994; 63: pp. 345–82. 2. Katsumoto, T. et al: The role of the vimentin intermediate filaments in rat 3Y1 cells elucidated by immunoelectron microscopy and computer-graphic reconstruction. Biol Cell,1990; 68 (2): pp. 139–46. 3. Zhao Y. et al: Vimentin affects the mobility and invasiveness of prostate cancer cells. Cell Biochem Funct. 2008 Sep-Oct;26(5):571-7.
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1.若都为正常,那么并未有太大问题,只要定期复查就可以了。
2.如果TGAb、TMAb、甲状腺功能均高,那么就是桥本氏甲状腺炎并甲亢,需要抗甲亢治疗。
3.如果TGAb、TMAb高,甲状腺功能下降,那么就是桥本氏甲状腺炎并甲减,需要进行甲减治疗。
4.如果TGAb、TMAb高,甲状腺功能正常,那么就是桥本氏甲状腺炎,无需特殊治疗,只要定期复查甲状腺功能就可能以了,不过这种情况有可能以后演变成甲减或者甲亢。
BAS-ELISA是在常规ELISA原理的基础上,结合生物素(B)与亲和素(A)间的高度放大作用,而建立的一种检测系统。生物素很易与蛋白质(如抗体等)以共价键结合。这样,结合了酶的亲和素分子与结合有特异性抗体的生物素分子产生反应,既起到了多级放大作用,又由于酶在遇到相应底物时的催化作用而呈色,达到检测未知抗原(或抗体)分子的目的。
1.若都为正常,那么并未有太大问题,只要定期复查就可以了。
2.如果TGAb、TMAb、甲状腺功能均高,那么就是桥本氏甲状腺炎并甲亢,需要抗甲亢治疗。
3.如果TGAb、TMAb高,甲状腺功能下降,那么就是桥本氏甲状腺炎并甲减,需要进行甲减治疗。
4.如果TGAb、TMAb高,甲状腺功能正常,那么就是桥本氏甲状腺炎,无需特殊治疗,只要定期复查甲状腺功能就可能以了,不过这种情况有可能以后演变成甲减或者甲亢。
试题分析:酶是一种生物催化剂,在催化反应中本身不发生变化;此非酶的特性;B项体现了酶的作用条件较温和的特性;C项体现了酶的专一性;D项体现了酶的高效性;故选A。
考点:本题考查的是酶的特性。
点评:对于此类试题,学生应掌握酶的特性。

