| Predicted Species Reactivity | Mouse |
|---|---|
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB |
| Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | The immunogen is a synthetic peptide directed towards the middle region of mouse GAMT |
| Purification | Affinity purified |
| Peptide Sequence | Synthetic peptide located within the following region: APTLPDGHFDGILYDTYPLSEEAWHTHQFNFIKNHAFRLLKTGGVLTYCN |
| Concentration | Batch dependent within range: 100 ul at 0.5 - 1 mg/ml |
| Blocking Peptide | For anti-GAMT (ARP90663_P050) antibody is Catalog # AAP90663 |
| Datasheets/Manuals | Printable datasheet for anti-GAMT (ARP90663_P050) antibody |
| Gene Symbol | GAMT |
|---|---|
| Official Gene Full Name | guanidinoacetate methyltransferase |
| Alias Symbols | Spintz1, AA571402 |
| NCBI Gene Id | 14431 |
| Protein Name | guanidinoacetate N-methyltransferase |
| Description of Target | Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development. |
| Swissprot Id | O35969-2 |
| Protein Accession # | NP_034385.1 |
| Nucleotide Accession # | NM_010255.3 |
| Protein Size (# AA) | 252 |
| Molecular Weight | 27 kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express GAMT.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express GAMT.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "GAMT Antibody - middle region (ARP90663_P050)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".
How long will it take to receive "GAMT Antibody - middle region (ARP90663_P050)"?
This item is available "Domestic: within 24 hours delivery | International: 3-5 days".
What buffer format is "GAMT Antibody - middle region (ARP90663_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "GAMT Antibody - middle region (ARP90663_P050)"?
This target may also be called "Spintz1, AA571402" in publications.
What is the shipping cost for "GAMT Antibody - middle region (ARP90663_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "GAMT Antibody - middle region (ARP90663_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "GAMT Antibody - middle region (ARP90663_P050)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "27 kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "GAMT Antibody - middle region (ARP90663_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "GAMT"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "GAMT"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "GAMT"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "GAMT"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "GAMT"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "GAMT"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
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1.若都为正常，那么并未有太大问题，只要定期复查就可以了。
2.如果TGAb、TMAb、甲状腺功能均高，那么就是桥本氏甲状腺炎并甲亢，需要抗甲亢治疗。
3.如果TGAb、TMAb高，甲状腺功能下降，那么就是桥本氏甲状腺炎并甲减，需要进行甲减治疗。
4.如果TGAb、TMAb高，甲状腺功能正常，那么就是桥本氏甲状腺炎，无需特殊治疗，只要定期复查甲状腺功能就可能以了，不过这种情况有可能以后演变成甲减或者甲亢。
BAS-ELISA是在常规ELISA原理的基础上，结合生物素(B)与亲和素(A)间的高度放大作用，而建立的一种检测系统。生物素很易与蛋白质(如抗体等)以共价键结合。这样，结合了酶的亲和素分子与结合有特异性抗体的生物素分子产生反应，既起到了多级放大作用，又由于酶在遇到相应底物时的催化作用而呈色，达到检测未知抗原(或抗体)分子的目的。
辣根过氧化物酶（Horseradish Peroxidase,HRP）比活性高，稳定，分子量小，纯酶容易制备，所以最常用。HRP广泛分布于植物界，辣根中含量高，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，分子量为40,000，等电点为PH3～9，酶催化的最适PH因供氢体不同而稍有差异，但多在PH5左右。酶溶于水和58%以下饱和度硫酸铵溶液。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm /OD275nm的比值RZ（德文Reinheit Zahl）表示酶的纯度。高纯度的酶RZ值应在3.0左右（最高可达3.4）。RZ值越小，非酶蛋白就越多。 1） RZ>3 活性 >250u/mg， 主要应用于免疫学，是高纯度的过氧化酶。采用特殊色谱纯化技术以除去会影响免疫学反应的同工酶B .
2） RZ>2 活性 >180u/mg ，主要应用于临床化学，我们的客户也有将这个规格的产品应用于免疫学研究的。此时，一个标准化的分析方法就变得尤为重要。
3） RZ>1 活性 >100u/mg，主要应用于血糖试纸和尿液分析试纸。
4） RZ>0.6 活性 >60u/mg ，主要应用于尿液分析试纸。向左转|向右转
英文名称：horseradish peroxidase;HRP
定义：一种糖蛋白，由于在辣根中该酶的含量很高，故名。它以铁卟啉为辅基，在过氧化氢存在时能催化苯酚、苯胺及其取代物聚合。
