


Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.

ThisantibodyisshippedwithitsantigenFREEofcharge!
- PeptideRTSDSRDHTRVDWKR(C),correspondingtoaminoacidresidues271-285ofratGluR1 (Accession P19490).Extracellular,N-terminus.
- Westernblotanalysis ofrat(lanes1and3)andmouse(lanes2and4)brainlysates:1,2.Anti-GluR1(GluA1)(extracellular)Antibody (#AGC-004), (1:200).
3,4.Anti-GluR1(GluA1)(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
- ExpressionofGluR1inmousehippocampusImmunohistochemicalstainingofmousehippocampus withAnti-GluR1(GluA1)(extracellular)Antibody (#AGC-004).GluR1(green)ispresentinthestratumoriens(Or)andrADIatum(Ra)butnotinthepyramidallayer(P).Stainingofthesamesectionwithmouseanti-parvalbumin(red)identifiesthepyramidallayer.
- Rathippocampalneurons(16μg/ml)(Verpelli,C. etal. (2011) J.Biol.Chem. 286, 34839.).
- 1.Dingledine,R.etal.(1999)Pharmacol.Rev.51,7.
- 2.Sheng,M.andLee,S.H.(2001)Cell105,825.
- 3.Song,I.andHuganir,R.L.(2002)TrendsNeurosci.25,578.
AMPAreceptors aremembersofthe glutamatereceptorfamily ofionchannelsthatalsoincludetheNMDAand Kainate receptors.ThethreesubfamiliesarenamedaftertheoriginalsyntheticagoNISTsthatwereidentifiedasselectiveligandsofeachfamily.
Theα-amino-3-hydroxy-5-methyl-4-isoazolepropionicacid(AMPA)receptorsubfamilyincludesfourmembersAMPA1-AMPA4thatarealsoknownasGluR1-GluR4respectively.
ThefunctionalAMPAchannelisbelievedtobeatetramer,withmostneuronalAMPAreceptorsbeingactuallyheterotetramerscomposedofAMPA1plus AMPA2 orAMPA2plus AMPA3,althoughhomotetramerscanalsobefound.
AMPAreceptorsarepermeabletocationsNa+,K+ andCa2+.TheCa2+ permeABIlityisdependentonthepresenceofAMPA2:wheneverthissubunitispresent,thechannelwillbeimpermeabletoCa2+.TheCa2+ permeabilityoftheAMPA2subunitisdeterminedbythepresenceofanarginine(R)atacriticalsiteintheporeloopinsteadofaglutamine(Q)presentinthesamesiteintheotherAMPAsubunits.Apost-transcriptionalprocessknownasRNAeditingdeterminesthepresenceofthisR.SincemostAMPA2subunitsintheadultbrainhaveundergoneRNAeditingandmostAMPAreceptorscontaintheAMPA2subunit, mostnativeAMPAreceptorswillbeimpermeabletoCa2+.
GatingofAMPAreceptorsbyglutamateisextremelyfastandthereforetheAMPAreceptorsmediatemostexcitatory(depolarizing)currentsinthebrainduringbasalneuronalactivity.Thedepolarizationcausedbytheactivationofpost-synapticAMPAreceptorsisnecessaryfortheactivationofNMDAreceptorsthatwillopenonlyinthepresenceofbothglutamateandadepolarizedmembrane.
Synapticstrength,definedasthelevelofpost-synapticdepolarization,canbelongterm(hencethetermlongtermpotentiation,LTP)andthereforeinducechangesinsignalingandproteinsynthesisintheactivatedneuron.Thesechangesareassociatedwithmemoryformationandlearning.
ChangesinsynapticstrengtharethoughttoinvolverapidmovementoftheAMPAreceptorsinandoutofthesynapsesandagreatdealofefforthasfocusedinunderstandingthemechanismsthatgovernAMPAreceptortrafficking.
Anti-GluR1(GluA1)(extracellular)Antibody(#AGC-004)isahighlyspecific antibodydirectedagainstanextracellularepitopeoftherationotropicglutamatereceptor1.Theantibody canbeusedinwesternblot,immunohistochemistry,immunocytochemistry,andlivecellimagingapplications.IthasbeendesignedtorecognizeGluR1fromhuman,mouse,andratsamples.
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1.若都为正常,那么并未有太大问题,只要定期复查就可以了。
2.如果TGAb、TMAb、甲状腺功能均高,那么就是桥本氏甲状腺炎并甲亢,需要抗甲亢治疗。
3.如果TGAb、TMAb高,甲状腺功能下降,那么就是桥本氏甲状腺炎并甲减,需要进行甲减治疗。
4.如果TGAb、TMAb高,甲状腺功能正常,那么就是桥本氏甲状腺炎,无需特殊治疗,只要定期复查甲状腺功能就可能以了,不过这种情况有可能以后演变成甲减或者甲亢。
1.若都为正常,那么并未有太大问题,只要定期复查就可以了。
2.如果TGAb、TMAb、甲状腺功能均高,那么就是桥本氏甲状腺炎并甲亢,需要抗甲亢治疗。
3.如果TGAb、TMAb高,甲状腺功能下降,那么就是桥本氏甲状腺炎并甲减,需要进行甲减治疗。
4.如果TGAb、TMAb高,甲状腺功能正常,那么就是桥本氏甲状腺炎,无需特殊治疗,只要定期复查甲状腺功能就可能以了,不过这种情况有可能以后演变成甲减或者甲亢。
英文名称:horseradish peroxidase;HRP
定义:一种糖蛋白,由于在辣根中该酶的含量很高,故名。它以铁卟啉为辅基,在过氧化氢存在时能催化苯酚、苯胺及其取代物聚合。

