质粒小提试剂盒使用说明书
来自 : mayitao
抑制素βE(INHβE)重组蛋白RecombinantInhibinBetaE(INHbE)
FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!
OrganismspeciesHomosapiens(Human)ProductNo.xy048Hu01SourceProkaryoticexpressionHostE.coliPurity>95%UOM50ugPredictedMolecularMass14.0kDaConcentration6.2ApplicationsSDS-PAGE;WB;ELISA;IP.EndotoxinLevel<1.0EUper1µg(determinedbytheLALmethod)SEQUENCES
抑制素βE(INHβE)重组蛋白ThetargetproteinisfusedwithN-terminalHis-Tag,itssequenceislistedbelow.
MGHHHHHHSGSEF-TPTCEPATPLCCRRDHYVDFQELGWRDWILQPEGYQLNYCSGQCPPHLAGSPGIAASFHSAVFSLLKANNPWPASTSCCVPTARRPLSLLYLDHNGNVVKTDVPDMVVEACGCSUSAGE
ReconstituteinsterilePBS,pH7.2-pH7.4.
STORAGEANDSTABILITY
抑制素βE(INHβE)重组蛋白Storage:Avoidrepeatedfreeze/thawcycles.Storeat2-8oCforonemonth.Aliquotandstoreat-80oCfor12months.StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37oCfor48h,andnoobviousdegradationandprecipitationwereobserved.(ReferringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinislessthan5%withintheexpirationdateunderappropriatestoragecondition.AbouttheMarker(complimentary)
抑制素βE(INHβE)重组蛋白EffectiveSizeRange:10kDato70kDa.Proteinbands:10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand70kDa.Doubleintensitybands:The26kDa,18kDa,10kDabandsareatdoubleintensitytomakelocationandsizeapproximationofproteinsofinterestquickandeasy.Ready-to-use:Noneedtoheat,diluteoraddreducingagentsbeforeuse.FormulationSuppliedaslyophilizedforminPBS,pH7.4,containing5%trehalose,0.01%sarcosyl.
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