Source:Pichia.Pastoris
MolecularWeight:RecombinantOPG/Fccontains412aminoacidresidues,including180residuesfrommatureOPG(a.a22-201)and232residuesfromtheFcproteinofhumanIgG1,andhasacalculatedmolecularmassof46.5kDa.Asaresultofglycosylation,therecombinantOPG/Fcmigratesasa49kDaproteininSDS-PAGEunderreducingconditions.
Purity:>90%bySDS-PAGEandHPLCanalyses.
AASequence:OPG22-201:
ETFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYTDSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKHRSCPPGFGVVQAGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGLLLTQKGNATHDNICSGNSESTQKCGIDVTL
Fc232:
EPKSSDKTHTCPPCPAPEFEGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPTPIEKTISKAKGQREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
BiologicalActivity:Fullybiologicallyactivewhencomparedtothestandard.DeterminedbyitsabilitytoneutralizethestimulationofU937cellstreatedwith10ng/mlofsolubleRANKL.
Formulation:Lyophilizedfroma0.2μmfilteredconcentrated(0.5mg/ml)solutioninPBS,pH7.4.
Endotoxin:Lessthan1EU/μgofrHuOPG-FcasdeterminedbyLALmethod.
Reconstitution:Werecommendthatthisvialbebrieflycentrifugedpriortoopeningtobringthecontentstothebottom.Reconstituteinsteriledistilledwateroraqueousbuffercontaining0.1%BSAtoaconcentrationof0.1-1.0mg/mL.Stocksolutionsshouldbeapportionedintoworkingaliquotsandstoredat<-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:Thislyophilizedpreparationisstableat2-8°C,butshouldbekeptat-20°Cforlongtermstorage,preferablydesiccated.Uponreconstitution,thepreparationisstableforuptooneweekat2-8°C.Formaximalstability,apportionthereconstitutedpreparationintoworkingaliquotsandstoreat-20°Cto-70°C.Avoidrepeatedfreeze/thawcycles.
Usage:Thismaterialisusedforresearch,laboratoryorfurtherevaluationpurposes.NOTFORHUMANUSE.
HumanOsteoprotegerin
Osteoprotegerin(OPG)isamemberoftheTNFRsuperfamilythatcanactasadecoyreceptorforRANKL.BindingofsolubleOPGtosRANKLinhibitsosteoclastogenesisbyinterruptingthesignalingbetweenstromalcellsandosteoclasticprogenitorcells,therebyleadingtoexcessaccumulationofboneandcartilage.OPGisexpressedinawidevarietyoftissuesincludingadultheart,lung,kidney,liver,spleen,prostate,lymphnodeandbonemarrow.OPGissecretedbothasamonomericandadimericprotein.Itsprimarystructureconsistsofsevendistinctdomains,fourofwhichcorrespondstotheextracellularcysteine-richdomainsofTNFRproteinsandconstitutesthesolubleOPG.