流式细胞仪实验方法及常见问题分析
| 货号： | xy-C399Hu01 |
| 供应商： | 上海信裕 |
| 数量： | 1000 |
| 英文名： | RecombinantComplexin1(CPLX1) |
| 保存条件： | -20℃ |
RecombinantComplexin1(CPLX1)
FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!
| Organismspecies | Homosapiens(Human) |
| ProductNo. | xy-C399Hu01 |
| Source | Prokaryoticexpression |
| Host | E.coli |
| Purity | >95% |
| UOM | 50ug |
| PredictedMolecularMass | 18.7kDa |
| Concentration | n/a |
| Applications | SDS-PAGE;WB;ELISA;IP. |
| EndotoxinLevel | <1.0EUper1µg(determinedbytheLALmethod) |
| Residues | Met1~Lys134withtwoN-terminalTags,His-tagandT7-tag |
| Formulation | Suppliedaslyophilizedformin20mMTris,150mMNaCl,pH8.0,containing1mMEDTA,1mMDTT,0.01%sarcosyl,5%trehalose,andpreservative. |
复合素1(CPLX1)重组蛋白 SEQUENCES
Thesequenceofthetargetproteinislistedbelow.
MEFVMKQALGGATKDMGKMLGGDEEKDPDAAKKEEERQEALRQAEEERKAKYAKMEAEREAVRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEVEEEDESILDTVIKYLPGPLQDMLKK
MEFVMKQALGGATKDMGKMLGGDEEKDPDAAKKEEERQEALRQAEEERKAKYAKMEAEREAVRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEVEEEDESILDTVIKYLPGPLQDMLKK
USAGE
ReconstituteinsterileddH2O.
复合素1(CPLX1)重组蛋白 STORAGEANDSTABILITY
Storage: Avoidrepeatedfreeze/thawcycles.Storeat2-8oCforonemonth.Aliquotandstoreat-80oCfor12months.
StabilityTest: Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37oCfor48h,andnoobviousdegradationandprecipitationwereobserved.(ReferringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinislessthan5%withintheexpirationdateunderappropriatestoragecondition.
StabilityTest: Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37oCfor48h,andnoobviousdegradationandprecipitationwereobserved.(ReferringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinislessthan5%withintheexpirationdateunderappropriatestoragecondition.
EffectiveSizeRange: 10kDato70kDa.
Proteinbands: 10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand70kDa.
Doubleintensitybands: The26kDa,18kDa,10kDabandsareatdoubleintensitytomakelocationandsizeapproximationofproteinsofinterestquickandeasy.
Ready-to-use: Noneedtoheat,diluteoraddreducingagentsbeforeuse.
Proteinbands: 10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand70kDa.
Doubleintensitybands: The26kDa,18kDa,10kDabandsareatdoubleintensitytomakelocationandsizeapproximationofproteinsofinterestquickandeasy.
Ready-to-use: Noneedtoheat,diluteoraddreducingagentsbeforeuse.
温馨提示：不可用于临床治疗。
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发布于 ： 2016-10-22
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