
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 100841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
AAV1 | 100841-AAV1 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
AAV5 | 100841-AAV5 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
AAV9 | 100841-AAV9 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Vector typeMammalian Expression, AAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberUnknown
Gene/Insert
- Gene/Insert nameGCaMP6m
- Alt nameGCaMP3 variant 632
- SpeciesSynthetic
- Insert Size (bp)1353
- MutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T R392G
- PromoterSynapsin
Cloning Information
- Cloning methodUnknown
- 5′ sequencing primerunknown (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- pAAV.Syn.GCaMP6m.WPRE.SV40 (p2823)_Q.gb
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
- Articles Citing this Plasmid
- 2 References
Depositor Comments
This plasmid was previously available as pAAV.Syn.GCaMP6m.WPRE.SV40 (p2823) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.
Information for AAV1 (Catalog # 100841-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA.
Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV5 (Catalog # 100841-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA.
Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV9 (Catalog # 100841-AAV9)(Back to top)
Purpose
Ready-to-use AAV9 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA.
Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
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包埋机,又称生物组织包埋机或者石蜡包埋机,是对人体或动植物标本经脱水浸蜡后进行组织蜡块包埋,以供切片后作组织学诊断或研究的设备,适用于医学院校、医院病理科、医学科研单位、动植物科研单位和食品检测等部门使用。
二.包埋机作用及原理
由于生物样品各种组织成分不同,因此性质也各异,如软硬程度,疏松、致密程度,面积大小等都不相同,自然状态下要将它们切成十几微米甚至几个微米的薄片,几乎是办不到的。包埋的原理就是将某些特殊的支持物质浸入到组织块内部,利用支持物质的物理特性,如由液态转变成固态,使整个组织具有均匀一致的固态结构和足够的硬度,以利于用切片机制取极薄的切片。组织学切片技术中最常用的一种包埋方法是利用石蜡作为包埋剂,将浸过蜡的组织块置于石蜡内制成蜡块。
由于组织是柔软的,或局部的软硬不均,使用常规技术制作切片或超薄切片时制作厚薄均匀的切片是困难的,所以有必要用一定物质浸透组织内部,整个组织一样硬化,先将熔化的石蜡注入包埋托内(模具),而后用镊子将经过浸蜡的组织块从脱水盒中取出,放入包埋托中央,放在小冷台上,用镊子轻按组织块,以达到组织块平整,盖上脱水盒底,再加好石蜡,移至冷冻台上,待冷冻好后,卸下组织蜡块待切。?
包埋机不仅用于观察正常细胞组织的形态结构,在病理学和法医学等学科用以研究、观察及判断细胞组织的形态变化的主要方法,而且也已相当广泛地用于其他许多学科领域的研究中;教学中,为达到光镜下观察切片标本。包埋解决方案帮助您确保正确的组织定位、避免出现热损坏并创造出理想的组织块形状。
三.包埋机清洁注意事项
每次清洁仪器之前,要注意关闭仪器,并拔下电源插头。
处理清洁材料时,请遵守制造商的安全规定以及所在国家的现行实验室规定。
清洁期间,不得有任何液体渗入仪器内部。
为防止刮伤仪器表面,在任何情况下均不得使用带有锋利边缘的金属工具。
工作表面的清洁:
适合去除石蜡的所有实验室常用清洁产品(例如,Paraguard或二甲苯替代品)均可用于清洁工作区。
仪器和外部表面的清洁:
必要时,请用温和的家用清洁剂或肥皂水清洁喷漆的外表面,并用湿布擦拭。
避免有机溶剂长时间接触仪器表面。不得在喷漆表面使用二甲苯或丙酮。
版主tk57ml留言:
就这么从别人的网站复制粘贴过来的?
所以在考虑换一种蜡试试,因为几乎每天都要做石蜡切片,所以比较急,可是已经很长时间了也没找到合适的.
做组化的同学及老师们有没有自己用过,而且觉得好用的蜡推荐一下啊?
不甚感激啊!
如题。实验室只有冰冻切片机,但标本已制成石蜡包埋的标本,问能否用冰冻切片机来切?还是一定得用石蜡切片机切片?
全自动且可编程的轮转式切片机LeicaRM2265是徕卡病理系统的顶级产品。还可以将RM2265全自动轮转式切片机与LeicaLN22冷冻附件配合使用,进行超低温切片(-150°C)。
全自动包埋机LeicaEG1150
LeicaEG1150模块化组织包埋中心整合了两个独立组件,LeicaEG1150C冷台和LeicaEG1150H加热石蜡分配模块。
独立模块为安排包埋工作流程提供了灵活性,可从两个方向(从左往右或从右往左)任意选择,满足客户的个人需求。位置可调的卤素聚光灯能够提供亮光,甚至可以照亮整个工作区域。
一个可选择的可调放大镜能更容易地在活检组织和其他小标本中定位标本。
全自动染色机LeicaST5010AutostainerXL
成熟的LeicaAutostainerXL始终为您提供可重复的、稳定的高品质染色并提高工作生产量。
此外,与LeicaCV5030玻片盖片机配套使用可建立一个工作站点,不再需要手动操作染色和盖片。
开放式耗材和试剂所带来的灵活性以及可选择使用经验证的Surgipath试剂,使各种耗材和方案能够提供出众的染色和盖片质量。
更多病理设备登录leica官网了解http://www.leicABIosystems.com/cn/
咨询徕卡病理官方请登录http://china.leicabiosystems.com/lbs_specimenpreparation/
最近在做小鼠结肠组织的石蜡包埋,试验过一次,包了四块石蜡,就一块是竖着的,其他都倒了,我需要肠的横切面,各位大侠给个注意。

