Highlights
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
Description
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
|---|---|
| Equipment | Microcentrifuge, vortex, magstand |
| Sample Inactivation | TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. |
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 10 µg RNA (binding capacity), ≥ 30 µl (elution volume) |
Q1: Is Direct-zol suitable for very small numbers of cells?
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q2: Is DNase I available for individual purchase?
All kit components are available for purchase separately.
Q3: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q4: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q5: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Q6: Is it possible to extract proteins with the Direct-zol RNA kits?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
Q10: Which phenol-based reagents are compatible with Direct-zol?
The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
“Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME!It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”
-A. Newhart (The Wistar Institute)
“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”
-Mohan K. (University of Illinois, Chicago)
“Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”
-Arjan V. (Indiana University)
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D4100-2-3 | MagBinding Beads | 3 ml | $66.00 | |
| D4100-2-12 | MagBinding Beads | 12 ml | $125.00 | |
| W1001-30 | DNase/RNase-Free Water | 30 ml | $22.00 | |
| R1060-2-100 | RNA Prep Buffer | 100 ml | $122.00 | |
| R1060-2-25 | RNA Prep Buffer | 25 ml | $40.00 | |
| R2100-1-20 | Direct-zol Binding Buffer Concentrate | 20 ml | $84.00 | |
| R2100-2-200 | Direct-zol MagBead PreWash | 200 ml | $174.00 | |
| R2130-1-120 | MagBead DNA/RNA Wash 1 | 120 ml | $198.00 | |
| R2130-1-30 | MagBead DNA/RNA Wash 1 | 30 ml | $63.00 | |
| R2130-2-20 | MagBead DNA/RNA Wash 2 | 20 ml | $54.00 | |
| R2130-2-80 | MagBead DNA/RNA Wash 2 | 80 ml | $171.00 | |
| C2002 | Collection Plate | 2 Plates | $22.00 | |
| C2003 | Elution Plate | 2 Plates | $19.00 | |
| C2007-8 | 96-Well Plate Cover Foil | 8 Foils | $18.00 | |
| R2050-1-200 | TRI Reagent | 200 ml | $219.00 | |
| E1010-1-4 | DNA Digestion Buffer | 4 mL | $15.00 | |
| E1010 | DNase I Set | 250 U | $56.00 |
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排除方法：
1．检查电源保证三相正常；和相序正常；
2．测量电压，把电压调整正常；
3．修理、更换接触器、排除线路故障。
4．更换按钮开关
故障2：液压系统压力过低或油缸不动作
排除方法：
1．把电动机转向纠正；
2．修理或更换油泵；
3．把系统压力调整正确；
4．检查吸油管路，更换密封件，排除渗漏；
5．加油至油标中位；
6．清洗或更换吸油或回油滤芯；
7．把油缸接头处的软管拧松，来回运动排气；
8．按要求更换液压油。
故障3：喂料减速机无动作或动作不稳定
排除方法：
1．检查电源保证三相正常；和相序正常；
2．测量电压，把电压调整正常；
3．修理、更换接触器、排除线路故障。
2、装机功率：MB达到50-85KW; MB135KW，而HTC虽然也是75KW，但是将砂浆泵的功率设为0.75KW,根据这一要求，更是将其使用辅料的范围直接限定成了只能用日本的切割液，并且是用日本的“619”配方的硅片切割液，对用户来说带来了很大的麻烦。由于HTC的装机功率极其的小，如果用国内的太阳能硅片切割液，就会因为液的粘度太大，无法协调硅片切割液和碳化硅微粉的配比比例和砂浆粘度的问题，除非切割液厂家为其专供。
3、对切割原料的要求：
MB等机器，要求硅片切割液和碳化硅微粉的配比比例一般控制在1：0.92-0.95，砂浆密度在1.630-1.635就可以切的相当理想。即便出现配比比例更大，甚至砂浆密度达到1.67左右都照样不会有什么问题，只要砂浆粘度控制在200--250就可以。但是HTC要求砂浆密度不能高于1.57，就是说只能控制在1.55-1.57.砂浆粘度在150左右，这样国内的硅片切割液液就会出现砂浆密度配在1.57，可能砂浆粘度还不到120，而如果把砂浆粘度调到150，密度就超过了1.57，甚至超过了1.60。砂浆粘度过大，直接的说法是会导致机器报警，其实更深层次的影响还有可能会导致片子洗不干净，出现灼伤片，或者电机发热，对机器本身的轴承有很大的磨损。
切片机是切制薄而均匀组织片的机械，组织用坚硬的石蜡或其他物质支持，每切一次借切片厚度器自动向前（向刀的方向）推进所需距离，厚度器的梯度通常为1微米。切制石蜡包埋的组织时，由于与前一张切片的蜡边粘着，而制成多张切片的切片条。
不同的切片机切片的方法也不同，比如试验中要处理细胞或者组织，从而方便用显微镜进行观察实验。用于光学显微镜的有旋转式和滑走式切片机
在造纸行业也需要用到切片机，适用刀盘式切片机，鼓式切片机，螺旋切片机等。刀盘式切片机由刀盘、机壳、喂料槽以及传动装置等部分组成，工作原理是利用沉重的刀盘起到飞轮的作用，稳定切片。
还有一种是将聚合物带条切成颗粒。这就需要一个特殊的切片机，这种切片机由导条板、喂入辊、加压辊、旋转刀盘组成。工作原理是：利用刀盘由无级变速器传动，喂入辊由刀盘通过一组变换齿轮传动，而刀盘按切粒尺寸装有多把刀片。可以自行调换变换齿轮可改变切断长度和无级变速器可改变带条的惟入速度。向左转|向右转
谢谢！
