주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
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제품특징
□ 제품설명
Inorganic pyrophosphate (also referred toas diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number ofdifferent enzymatic reactions. Enzymes that utilize PPi as a substrate mayinclude phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymesthat generate PPi are more numerous and may include cyclases (EC 4.6.1),hydrolyses (EC 3) and ligases (EC 6). The PPiLight™ Assay is a non-radioactivebioluminescent assay for the detection of inorganic pyrophosphate. In thepresence of PPi the detection reagent catalyses the conversion of AMP to ATP.The assay uses luciferase, which produces light from the newly formed ATP andluciferin.
Figure 1: Bioluminescent reaction
□ 특장점
● Fast - Measure enzyme activityvia pyrophosphate consumption in 1 hour
● Simple - The 2 step,luminescent assay doesn’t require radioactive substrates,
beads, detectionantibodies or modified substrates
● Wide Detection Range - Sensitiveto 0.02 μM with a linear range to 10 μM
● Versatile - Scalable to 96-,384-, 1586-well formats
□ Applications
Measure activity of:
● Phosphotransferases
● Pyrophosphotases
● Cyclases
● Ligases
● Hydrolases
● DNA Polymerases
□ 보존
2°C - 8°C
ebiomall.com
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1：使用时可按需要的容量加水，以缩短加热的时间。（注意加水不可过多，以免沸腾时水溢出锅外，并注意锅内水量不能低于水箱高度的三分之二，水量过低会造成温冲过高测温不准。长时间使用需经常加水，不能使锅内电热管露出水面。以免烧坏电热管，造成漏水）。接通电源，打开电源开关，指示灯亮.
2. 通电后先闪烁显示dfs,后显示日.以检测数码管笔画是否有缺.
3. 设定温度：按设定键SET设定指示灯SL亮,进入温度设定状态,按上调键▲向上调整0.1℃。按下调键 ▼向下调整0.1℃。再按一次SET键回到测量状态。
4. 调整加热功率系数：长按设置键SET5-8秒，首位显示d.进入设定最低加热功率系数,开机自动为3.可在1-9调整.（使用70-99.9℃时可将加热功率系数调整为5-9即可）。
5. 误差的修正：在确认仪表显示的值不是正确的测量值时可对显示值进行修正。按SET键5-8
秒进入仪表内层菜单，第一个出现的参数为首位显示P.进入设定最低加热功率系数,再按一次SET键出现并的参数C即误差修正参数，配合△或▽键可修改此参数。误差的修正的范围为-9.9℃到+9.9℃，（例如：控温仪显示温度37℃，水箱内的实际温度36℃，则C值减少1℃。 如果控温仪显示温度37℃，水箱内的实际温度38℃，则C值增加1℃。）修正完成后再按一下SET键退出。仪表出厂时C值已修正，使用时要防止把显示正确的仪表修正至不正确。
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实验室自己做滴丸，基质用的PEG4000和PEG6000混合，80℃在水浴锅上熔融，只要基质离开水浴锅就会冷凝，胶头滴管会堵住，滴不出来，是什么原因，怎么解决，各位大神看到帮帮忙
