TEER Measurement with automatic data logging
- Low noise design offers greater resolution and accuracy
- Automatic 20X sample averaging improves accuracyand stability
- Adjustable fixed measurement currents (2, 4 or 10 μA)
- Resistance auto ranging from 1 Ω to 100,000 Ω or withthree fixed current ranges
- Reliable low current, low voltage design prevents metalion transport
- Fast resistance stabilization on low levels under 200 Ωwith resolution to 0.1 Ω
- Ergonomic tilt stand for low glare operation
- Graphical display of popular plates (6, 12, 24, 96) fortrend analysis
- The display shows the most recent set of parameters
- Automatic plate indexing operation with or withoutcontrol well subtraction for resistance and potentialdifference (PD) measurements
- Continuous data logging via USB (PC, Mac, Linux)
- Saves date stamped data to a spreadsheet readablefile on a USB drive
- Upgradable firmware
Benefits
- Eliminates errors and reduces experimentalprocessing time
- Auto data logging eliminates the need totrack data by hand
- The small footprint allows more benchspace
- Easy calibration and verification
- Footswitch for hands-free recording
- Prevent data loss with auto save and datarecovery when battery is low
- TEER is easily computed by applying a unitarea formula to the resistance
Applications
- Measure epithelial or endothelial tissues forconfluence, TEER and potential difference
- Permeability, conductance and drug studies
- Continuous digital monitoring of a target membrane
- Common studies
- Blood-brain barrier transport
- Lung epithelial tissue studies
- Intestinal tissue studies
- Skin studies
EVOM3 For TeerMeasurement
The EVOM3 delivers improved workflow efficiency, more stable and repeatablemeasurements versus traditional Trans Epithelial Electrical Resistance (TEER) meters.Providing users with vital feedback during experiment measurements, the EVOM3’s large screenoffers a range of informational views. The new graphical displays for trend analysis and measurement values helpsscientists deliver simple, stepwise methodology during experimental measurements. The touch screen interface providesusers an intuitive, easy-to-use menu for configuration.Eliminating the need to log data by hand, the EVOM3 writes the resistance or voltage information to a USB drive in CSVformat for easy transfer to spreadsheets and data analysis programs. When used with the footswitch it enables handsfreerecording of measurements.At the heart of the EVOM3 is our latest processor and circuitry, providing users with quick, easy and reliable readingsdue to its fast stabilization, automatic twenty times sampling average and low noise design. The auto ranging resistancefeature allows for fast resistance measurements, and an over-range display feature eliminates false readings. The EVOM3has adjustable current levels in three fixed ranges with two lower ranges for sensitive membranes and high resistanceranges up to 100 KΩ.
TEER Measurement Electrode
The STX2-PLUS electrode was designed for easy insertion into many 24-well plates. Itis location re-placeable in the insert for repeatable and consistent measurements. Thenew shielded electrodes are now designed to minimize electrical interference and to bemore easily maintained.
- STX2-PLUS new electrode designed for 12 and 24-wellplates.
- Weighted self-standing electrode for hands-free stablemeasurements
- Shielded cable to minimize electrical and cellphone interference
System Components
| What is included with the EVOM3 | QTY |
| EVOM3 Epithelial Volt Ohm Meter | 1 |
| STX2-PLUS Electrode set | 1 |
| 300749 USB drive 32 GB (Used for storage.Also contains a Python 3.8 program forcontinuous digital monitoring of a target insert). | 1 |
| 503535 USB cable | 1 |
| 99673 Calibration kit, 1000Ω Test Resistor | 1 |
| 803025 A/C power cord and charger | 1 |
| 13142 Foot switch | 1 |
NOTE: A 99672 EVOM2 to EVOM3 Electrode Adapter is soldseparately. The STX2, STX3 and all STX100s require the use ofthis adapter with the EVOM3.
STX2-Plus
STX2-PlusBenefits
- Keyed electrode base for repeatable placementgives more consistent results, eliminating theneed for multiple readings.
- Easy to maintain
How Does the EVOM3 Work?
Confluence of a cellular monolayer is determined by an increase or a plateau in tissue resistance detected using theunique electronic circuit of the EVOM3 and the new STX2-PLUS electrode. The EVOM3 qualitatively measures cellmonolayer health and quantitatively measures cell confluence. The EVOM3 produces a low AC current that avoidselectrode metal deposits and adverse effects on tissues which can otherwise be caused by higher DC currents. TheEVOM3 uses low current and voltages and is designed for non-destructive testing for epithelial monolayer confluencein cell cultures. In addition, resistance readings are unaffected by membrane capacitance or membrane voltage. Theaccuracy and repeatability of the EVOM3-STX2-PLUS system makes this instrument ideal for permeability, PD and otherdetailed membrane studies.
Electrodes For TEER (Epithelial) Measurement
| Part # | Descriptions |
| STX2-PLUS | Replacement Electrode Set |
| STX2* | Replacement Electrode Set (Requires 99672 foruse with the EVOM3) |
| STX3* | Adjustable electrode set for shallow wells, 5-9 mmdepth |
| 3993* | 2 mm Adapter for EVOM2 |
*(Requires 99672 for use with the EVOM3)
ENDOHM Chambers For Endothelial/EpithelialMeasurement
NEW EndOhm chambers include the EVOM3 cable 99916.
| Part # | Descriptions |
| ENDOHM-6G | EndOhm for 6 mm culture cup (24 wells perplate) |
| ENDOHM-12G | EndOhm for 12 mm culture cup (12 wellsper plate) |
| ENDOHM-24G | EndOhm for 24 mm and Costar Snapwellcup (6 wells per plate) |
TEER measurement techniques for in vitro barrier model systems
EVOM3 Brochure
Quick Start Guide
EVOM3 Instruction Manual
STX2-PLUS Instruction Manual
This unit conforms to the following specifications:
| Type | Descriptions |
| Tissue Sampling Frequency | 12.5 Hz |
| Sample Averaging | 20 samples per second |
| Resistance Ranges |
|
| Auto Mode | 1 to 100,000 Ω auto current 2 μA, 4 μA, 10 μA |
| Resistance Resolution | 0.1 Ω (under 200 Ω); 1 Ω (over 200 Ω) |
| Resistance Accuracy |
|
| Voltage Resolution | 0.001 V, 0.1 mV |
| Accuracy Resistance | 0.1 Ω (200 Ω); 1 Ω (above 200 Ω) |
| Accuracy voltage | ± 0.1 mV |
| Current Levels |
|
| Display Update Rate | 0.5 seconds |
| Battery | 3.7V Li-ion 2500 mAh** |
| Charging Period | 5.5 hours (power off); 6 hours (run time) |
| Charge Current | 200 mA |
| Power Consumption | ~250 mA |
| Certifications | CE |
**mAH means milliamp hours.
Stanifer, M. L., et al. (2016). Reovirus intermediate subviral particlesconstitute a strategy to infect intestinal epithelial cells by exploiting TGF-βdependent pro-survival signaling. Cellular Microbiology, 18(12), 1831–1845.https://doi.org/10.1111/cmi.12626Meenach, S. A., et al. (2016). Development of three-dimensional lungmulticellular spheroids in air- and liquid-interface culture for the evaluationof anticancer therapeutics. International Journal of Oncology, 48(4), 1701–1709. https://doi.org/10.3892/ijo.2016.3376Ferguson, M. C., et al. (2015). Ability of the Encephalitic Arbovirus SemlikiForest Virus To Cross the Blood-Brain Barrier Is Determined by the Chargeof the E2 Glycoprotein. Journal of Virology, 89(15), 7536–7549. https://doi.org/10.1128/JVI.03645-14
Optional Foot Switch for Micro2T, Micro-ePore™, DMF100013142For pricing, Customers outside of the US and Canada, please contact your distributor.
Electrode Set For EVOM399673For pricing, Customers outside of the US and Canada, please contact your distributor.
USB2 Cable503535For pricing, Customers outside of the US and Canada, please contact your distributor.
EVOM2 Mini XLR Adaptor99675For pricing, Customers outside of the US and Canada, please contact your distributor.
EVOM3 Legacy Adaptor99672For pricing, Customers outside of the US and Canada, please contact your distributor.
EVOM3 upgrade cable USB Mini-B803026For pricing, Customers outside of the US and Canada, please contact your distributor.
EVOM3 EndOhm cable99916For pricing, Customers outside of the US and Canada, please contact your distributor.
USB Drive 32GBMultiple SKUs
ebiomall.com
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医用细胞涂片离心机
医用细胞离心涂片机
医用细胞离心涂片机是第一款使用精密控制的离心技术对细胞进行分离，形成薄层同时还能保持细胞完整的设备。它采用离心原理，将细胞
转移到诊断载玻片的固定区域，样品残液则被过滤卡吸收。通过离心力的作用，细胞结构趋向扁平，故细胞核表达尤为清晰。各种细胞在离
心过程中机会均等，不存在某些细胞或成份被人为过滤的情况，故最能反映细胞的真实环境状态。医用细胞离心涂片机特殊的倾斜结构设计还可避免细
胞在制备过程中丢失
医用细胞离心涂片机的主要特点
●医用细胞离心涂片适用于所有液基样本，尤其是细胞含量低的液基样本，如脑脊液、尿液
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●医用细胞离心涂片医用细胞离心涂片带自锁功能的塑料外盖，且仅有在盖门上锁的情况下，仪器方能进入运行状态
●医用细胞离心涂片标配一个密封舱，可高温高压消毒
●医用细胞离心涂片3级加速控制，保护脆性样品
●符合ICE61010的离心安全标准
●样本安全警报：结束后每分钟报警一次，提醒客户及时取出样本，防止风干
●可选择一次性使用耗材或反复多次使用耗材
医用细胞离心涂片适用于医院临床检验、生物、化学实验室和血站、血浆站对血液等样品的分离，是最新一代具有专门功能的产品。由微机控制，变频电机驱动，无碳刷粉尘污染，内腔由优质不锈钢制成，快速升降，操作简便，噪声低，振动小。
该系列离心机广泛适用于免疫血液学实验室、检验室、研究室，可进行红细胞血清学实验，做抗原、抗体的鉴定及库姆式实验的结果判断等，是各类医院血库，实验室，血站，医学院校和医学研究机构的必备设备。
医用细胞涂片离心机http://www.fameinstrument.cn/
普通生化检查分离血清，这个是用低速离心机转速在3000—3500转。一般在3分钟之内就可以了，分析分离效果，可以酌情调节转速和时间，用试验型的那种小的就行
至于管式离心机分离血清生产疫苗，这个有着严格的工艺规范，只能照做，不能随意更改参数
富一阳光与你交流离心机分离机设备相关问题，希望对你有帮助~
RCF = 1.119 x 10-5 x (rpm)2 x r
其中r 表示离心机转轴中心与离心管中心的距离，单位为cm。由于离心管的位置由转子（rotor）决定，因此r 必须由查阅相关转子的参数而得。
GB 19815-2005 离心机安全要求
GB 6065-1985 TZ立式振动离心机
GB 12258-1990 医用低速离心机
JB/T 3263-2000 卧式振动离心机
JB/T 4064-2005 上悬式离心机
JB/T 5519-1991高速冷冻离心机
JB/T 6118-1992 TCL沉降过滤离心机
JB/T 53190-1999 三足式离心机 产品质量分等
JB 447-1985卧式活塞推料离心机技术条件
JB/T 502-2004 螺旋卸料沉降离心机
JB/T 5284-1991 防爆型刮刀卸料离心机 序号 标 准 代 号 标 准 名 称 1 GB/T 4774-2004 分离机械 名词术语 2 GB 7779-2005 离心机型号编制方法 3 GB 10901-2005 离心机 性能测试方法 4 GB/19815-2005 离心机 安全要求 5 GB/T10894-2004 分离机械 噪音测试方法 6 GB/T10895-2004 离心机 分离机 机械振动测试方法 7 JB/T 447-2004 活塞推料离心机 8 JB/T 10411-2004 离心机、分离机奥氏体钢锻件超声检测及质量评级 9 JB/T 8051-2006 离心机转鼓强度计算规范 10 JB/T 8865-2001 活塞推料离心机用滤网 11 JB/T 9095-1999 离心机、分离机锻焊件无损探伤技术规范 12 JB/T 7217-2006 分离机械涂装通用技术条件 13 JB/T 6418-2006 分离机械 清洁度测定方法向左转|向右转
1.冷凝器积垢：冷凝器换热管内表水质积垢(开式循环的冷却水系统最容易积垢)，而导致传热热阻增大，换热效果降低，使冷凝温度升高或蒸发温度降低，另外，由于水质未经处理和维护不善，同样造成换热管内表面沉积沙土、杂质、藻类等物，造成冷凝压力升高而导致离心机喘振发生。
2.制冷系统有空气：当离心机组运行时，由于蒸发器和低压管路都处于真空状态，所以连接处极容易渗入空气，另外空气属不凝性气体，绝热指数很高，为1.4，当空气凝积在冷凝器上部时，造成冷凝压力和冷凝温度升高，而导致离心机喘振发生。
3.冷却塔冷却水循环量不足，进水温度过高等。由于冷却塔冷却效果不佳而造成冷凝压力过高，而导致喘振发生。
4.蒸发器蒸发温度过低：由于系统制冷剂不足、制冷量负荷减小，球阀开启度过小，造成蒸发压力过低而喘振。
5.关机时未关小导叶角度和降低离心机排气口压力。当离心机停机时，由于增压突然消失，蜗壳及冷凝器中的高压制冷剂蒸气倒灌，容易喘振。
离心机喘振排除
1.冷凝器结垢：清除传热面的污垢和清洗冷却塔。
2.系统中空气排除：离心机采用K11制冷剂时，一般液体温度超过28℃ 时，表明系统中有空气存在。排除方法：启动抽气回收装置，将不凝性气体排出，一般将制冷剂R11的压力抽到稍低于制冷荆液体温度相对应的饱和压力，即28℃以下的对应压力：117．68KMP以下即可。
3.启动后发生喘振：进行反喘振调节。当能量调节大幅度减少时，造成吸气量不足，即蒸气不能均匀流入叶轮，导致排气压力陡然下降，压缩机处于不稳定工作区，而发生喘振。为了防止喘振，可将一部分被压缩后的蒸气，由排气管旁通到蒸发器，不但可防喘振．而且对离心机启动时也有益：减少蒸气密度和启动时的压力，可减小启动功率。
4.蒸发压力过低：检查蒸发压力过低原因，制冷剂不足添加制冷剂，制冷量负荷小，关闭能量调节叶片。
5.停机时喘振：停离心机时应注意主电机有无反转现象，并尽可能关小导叶角度，降低离心机排气口压力。
离心机操作过程中，应保持冷凝压力和蒸发压力的稳定，使离心机制冷量高于喘振点对应制冷量，以防喘振
