

TEER Measurement with automatic data logging
- Low noise design offers greater resolution and accuracy
- Automatic 20X sample averaging improves accuracyand stability
- Adjustable fixed measurement currents (2, 4 or 10 μA)
- Resistance auto ranging from 1 Ω to 100,000 Ω or withthree fixed current ranges
- Reliable low current, low voltage design prevents metalion transport
- Fast resistance stabilization on low levels under 200 Ωwith resolution to 0.1 Ω
- Ergonomic tilt stand for low glare operation
- Graphical display of popular plates (6, 12, 24, 96) fortrend analysis
- The display shows the most recent set of parameters
- Automatic plate indexing operation with or withoutcontrol well subtraction for resistance and potentialdifference (PD) measurements
- Continuous data logging via USB (PC, Mac, Linux)
- Saves date stamped data to a spreadsheet readablefile on a USB drive
- Upgradable firmware
Benefits
- Eliminates errors and reduces experimentalprocessing time
- Auto data logging eliminates the need totrack data by hand
- The small footprint allows more benchspace
- Easy calibration and verification
- Footswitch for hands-free recording
- Prevent data loss with auto save and datarecovery when battery is low
- TEER is easily computed by applying a unitarea formula to the resistance
Applications
- Measure epithelial or endothelial tissues forconfluence, TEER and potential difference
- Permeability, conductance and drug studies
- Continuous digital monitoring of a target membrane
- Common studies
- Blood-brain barrier transport
- Lung epithelial tissue studies
- Intestinal tissue studies
- Skin studies
EVOM3 For TeerMeasurement
The EVOM3 delivers improved workflow efficiency, more stable and repeatablemeasurements versus traditional Trans Epithelial Electrical Resistance (TEER) meters.Providing users with vital feedback during experiment measurements, the EVOM3’s large screenoffers a range of informational views. The new graphical displays for trend analysis and measurement values helpsscientists deliver simple, stepwise methodology during experimental measurements. The touch screen interface providesusers an intuitive, easy-to-use menu for configuration.Eliminating the need to log data by hand, the EVOM3 writes the resistance or voltage information to a USB drive in CSVformat for easy transfer to spreadsheets and data analysis programs. When used with the footswitch it enables handsfreerecording of measurements.At the heart of the EVOM3 is our latest processor and circuitry, providing users with quick, easy and reliable readingsdue to its fast stabilization, automatic twenty times sampling average and low noise design. The auto ranging resistancefeature allows for fast resistance measurements, and an over-range display feature eliminates false readings. The EVOM3has adjustable current levels in three fixed ranges with two lower ranges for sensitive membranes and high resistanceranges up to 100 KΩ.
TEER Measurement Electrode
The STX2-PLUS electrode was designed for easy insertion into many 24-well plates. Itis location re-placeable in the insert for repeatable and consistent measurements. Thenew shielded electrodes are now designed to minimize electrical interference and to bemore easily maintained.
- STX2-PLUS new electrode designed for 12 and 24-wellplates.
- Weighted self-standing electrode for hands-free stablemeasurements
- Shielded cable to minimize electrical and cellphone interference
System Components
| What is included with the EVOM3 | QTY |
| EVOM3 Epithelial Volt Ohm Meter | 1 |
| STX2-PLUS Electrode set | 1 |
| 300749 USB drive 32 GB (Used for storage.Also contains a Python 3.8 program forcontinuous digital monitoring of a target insert). | 1 |
| 503535 USB cable | 1 |
| 99673 Calibration kit, 1000Ω Test Resistor | 1 |
| 803025 A/C power cord and charger | 1 |
| 13142 Foot switch | 1 |
NOTE: A 99672 EVOM2 to EVOM3 Electrode Adapter is soldseparately. The STX2, STX3 and all STX100s require the use ofthis adapter with the EVOM3.

STX2-Plus
STX2-PlusBenefits
- Keyed electrode base for repeatable placementgives more consistent results, eliminating theneed for multiple readings.
- Easy to maintain


How Does the EVOM3 Work?
Confluence of a cellular monolayer is determined by an increase or a plateau in tissue resistance detected using theunique electronic circuit of the EVOM3 and the new STX2-PLUS electrode. The EVOM3 qualitatively measures cellmonolayer health and quantitatively measures cell confluence. The EVOM3 produces a low AC current that avoidselectrode metal deposits and adverse effects on tissues which can otherwise be caused by higher DC currents. TheEVOM3 uses low current and voltages and is designed for non-destructive testing for epithelial monolayer confluencein cell cultures. In addition, resistance readings are unaffected by membrane capacitance or membrane voltage. Theaccuracy and repeatability of the EVOM3-STX2-PLUS system makes this instrument ideal for permeability, PD and otherdetailed membrane studies.
Electrodes For TEER (Epithelial) Measurement
| Part # | Descriptions |
| STX2-PLUS | Replacement Electrode Set |
| STX2* | Replacement Electrode Set (Requires 99672 foruse with the EVOM3) |
| STX3* | Adjustable electrode set for shallow wells, 5-9 mmdepth |
| 3993* | 2 mm Adapter for EVOM2 |
*(Requires 99672 for use with the EVOM3)
ENDOHM Chambers For Endothelial/EpithelialMeasurement
NEW EndOhm chambers include the EVOM3 cable 99916.
| Part # | Descriptions |
| ENDOHM-6G | EndOhm for 6 mm culture cup (24 wells perplate) |
| ENDOHM-12G | EndOhm for 12 mm culture cup (12 wellsper plate) |
| ENDOHM-24G | EndOhm for 24 mm and Costar Snapwellcup (6 wells per plate) |
TEER measurement techniques for in vitro barrier model systems
EVOM3 Brochure
Quick Start Guide
EVOM3 Instruction Manual
STX2-PLUS Instruction Manual
This unit conforms to the following specifications:
| Type | Descriptions |
| Tissue Sampling Frequency | 12.5 Hz |
| Sample Averaging | 20 samples per second |
| Resistance Ranges |
|
| Auto Mode | 1 to 100,000 Ω auto current 2 μA, 4 μA, 10 μA |
| Resistance Resolution | 0.1 Ω (under 200 Ω); 1 Ω (over 200 Ω) |
| Resistance Accuracy |
|
| Voltage Resolution | 0.001 V, 0.1 mV |
| Accuracy Resistance | 0.1 Ω (200 Ω); 1 Ω (above 200 Ω) |
| Accuracy voltage | ± 0.1 mV |
| Current Levels |
|
| Display Update Rate | 0.5 seconds |
| Battery | 3.7V Li-ion 2500 mAh** |
| Charging Period | 5.5 hours (power off); 6 hours (run time) |
| Charge Current | 200 mA |
| Power Consumption | ~250 mA |
| Certifications | CE |
**mAH means milliamp hours.
Stanifer, M. L., et al. (2016). Reovirus intermediate subviral particlesconstitute a strategy to infect intestinal epithelial cells by exploiting TGF-βdependent pro-survival signaling. Cellular Microbiology, 18(12), 1831–1845.https://doi.org/10.1111/cmi.12626Meenach, S. A., et al. (2016). Development of three-dimensional lungmulticellular spheroids in air- and liquid-interface culture for the evaluationof anticancer therapeutics. International Journal of Oncology, 48(4), 1701–1709. https://doi.org/10.3892/ijo.2016.3376Ferguson, M. C., et al. (2015). Ability of the Encephalitic Arbovirus SemlikiForest Virus To Cross the Blood-Brain Barrier Is Determined by the Chargeof the E2 Glycoprotein. Journal of Virology, 89(15), 7536–7549. https://doi.org/10.1128/JVI.03645-14
Optional Foot Switch for Micro2T, Micro-ePore™, DMF100013142For pricing, Customers outside of the US and Canada, please contact your distributor.
Electrode Set For EVOM399673For pricing, Customers outside of the US and Canada, please contact your distributor.
USB2 Cable503535For pricing, Customers outside of the US and Canada, please contact your distributor.
EVOM2 Mini XLR Adaptor99675For pricing, Customers outside of the US and Canada, please contact your distributor.
EVOM3 Legacy Adaptor99672For pricing, Customers outside of the US and Canada, please contact your distributor.
EVOM3 upgrade cable USB Mini-B803026For pricing, Customers outside of the US and Canada, please contact your distributor.
EVOM3 EndOhm cable99916For pricing, Customers outside of the US and Canada, please contact your distributor.
USB Drive 32GBMultiple SKUs
ebiomall.com
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靠转筒高速旋转,令里面的液体产生离心力分离或附在筒壁上。常用的衣服脱水机就可以看成是一种离心机。
一般用于煤焦油等脱水脱渣的设备,根据液体中介质的密度不同旋转后产生分层,从而起到分离的效果
离心机机械分离过程主要是物理过程,分离机械分离性能的优劣,与被分离物料的物理性能有极大的关系。如利用沉降原理进行分离的重力沉降或沉降式离心机与固相颗粒的粒径分布、固相密度、颗粒形状以及液相的密度、粘度和表面张力等均密切相关。各种沉降式分离机械的适应范围也依据物料的性质来划分。
步骤/方法1、机器裸露部分涂有防锈油脂,安装前应清除之。待安装完毕,重新涂上润滑油。然后将机器安装在混凝土基础或钢架上。安装过滤机时以两横梁为基础校正水平。
2、然后灌浇混凝土。机器周围要留有余地.考虑安装、拆卸管道以及出滤饼和检修等的方便。按要求接入管道、阀门等。根据您的机型和工艺需要配好管路。
3、按线路容量选好橡套软电缆,外套金属软管,分别接入L1、L2、L3和PEN。试运转;观察油泵电机(风叶从上端往下看)应顺时针旋转。
4、先安装压滤机的滤布,然后再安装滤板,这是比较正常的流程。关于滤布的选择:最好是根据悬浮液的性质对滤饼的要求选择滤布。
5、滤布的性能决定着过滤效果和滤布的使用寿命。滤布的结构:本机型滤布的结构为袖套式滤布。
6、压滤机的头板已被安装固定在压滤机的止推板上;尾板安装固定在压滤机的压紧板上。按要求品质的液压油,经20μm的滤网孔过滤后加入油箱内至视镜上限。
7、启动油泵将油压入液压缸,此时液位应位于视镜中线位。查看过滤机的压力表。表示数值应≤相应款式压滤机插页中所规定的液压工作压力的Mpa数。
8、如查证液压泵系统正常、压力表无误、压力不符合插页规定数值时,可调节压力。调节压力:使泄压压力大于液压工作压力约1Mpa。
9、电接点压力表上限调整至液压工作压力值,下限为液压工作压力减去2Mpa。检查并调整自动型压滤机的传动部分和拉板盒的正确位置。
注意事项(1)液相属易挥发性或易引起爆炸和易燃性物料时,不能用真空过滤,且要求密闭防爆。
(2)腐蚀性较强的物料,对分离机械材料的选择有特殊要求。
(3)固相颗粒硬度较大时,则要求所选分离机械材料的耐磨性好。
(4)被分离物料,无论是固相或液相,若属于贵重物料,则要求回收率高。
(5)固相物料为结晶产品时,要求分离时结晶的破损程度低。这对分离机械的结构及卸料方式、方法都有特殊要求。
普通生化检查分离血清,这个是用低速离心机转速在3000—3500转。一般在3分钟之内就可以了,分析分离效果,可以酌情调节转速和时间,用试验型的那种小的就行
至于管式离心机分离血清生产疫苗,这个有着严格的工艺规范,只能照做,不能随意更改参数
富一阳光与你交流离心机分离机设备相关问题,希望对你有帮助~
GB 19815-2005 离心机安全要求
GB 6065-1985 TZ立式振动离心机
GB 12258-1990 医用低速离心机
JB/T 3263-2000 卧式振动离心机
JB/T 4064-2005 上悬式离心机
JB/T 5519-1991高速冷冻离心机
JB/T 6118-1992 TCL沉降过滤离心机
JB/T 53190-1999 三足式离心机 产品质量分等
JB 447-1985卧式活塞推料离心机技术条件
JB/T 502-2004 螺旋卸料沉降离心机
JB/T 5284-1991 防爆型刮刀卸料离心机 序号 标 准 代 号 标 准 名 称 1 GB/T 4774-2004 分离机械 名词术语 2 GB 7779-2005 离心机型号编制方法 3 GB 10901-2005 离心机 性能测试方法 4 GB/19815-2005 离心机 安全要求 5 GB/T10894-2004 分离机械 噪音测试方法 6 GB/T10895-2004 离心机 分离机 机械振动测试方法 7 JB/T 447-2004 活塞推料离心机 8 JB/T 10411-2004 离心机、分离机奥氏体钢锻件超声检测及质量评级 9 JB/T 8051-2006 离心机转鼓强度计算规范 10 JB/T 8865-2001 活塞推料离心机用滤网 11 JB/T 9095-1999 离心机、分离机锻焊件无损探伤技术规范 12 JB/T 7217-2006 分离机械涂装通用技术条件 13 JB/T 6418-2006 分离机械 清洁度测定方法向左转|向右转
医用细胞涂片离心机
医用细胞离心涂片机
医用细胞离心涂片机是第一款使用精密控制的离心技术对细胞进行分离,形成薄层同时还能保持细胞完整的设备。它采用离心原理,将细胞
转移到诊断载玻片的固定区域,样品残液则被过滤卡吸收。通过离心力的作用,细胞结构趋向扁平,故细胞核表达尤为清晰。各种细胞在离
心过程中机会均等,不存在某些细胞或成份被人为过滤的情况,故最能反映细胞的真实环境状态。医用细胞离心涂片机特殊的倾斜结构设计还可避免细
胞在制备过程中丢失
医用细胞离心涂片机的主要特点
●医用细胞离心涂片适用于所有液基样本,尤其是细胞含量低的液基样本,如脑脊液、尿液
●医用细胞离心涂片同时处理12份样本
●医用细胞离心涂片优化的盖门开关,方便人员单手操作
●医用细胞离心涂片医用细胞离心涂片带自锁功能的塑料外盖,且仅有在盖门上锁的情况下,仪器方能进入运行状态
●医用细胞离心涂片标配一个密封舱,可高温高压消毒
●医用细胞离心涂片3级加速控制,保护脆性样品
●符合ICE61010的离心安全标准
●样本安全警报:结束后每分钟报警一次,提醒客户及时取出样本,防止风干
●可选择一次性使用耗材或反复多次使用耗材
医用细胞离心涂片适用于医院临床检验、生物、化学实验室和血站、血浆站对血液等样品的分离,是最新一代具有专门功能的产品。由微机控制,变频电机驱动,无碳刷粉尘污染,内腔由优质不锈钢制成,快速升降,操作简便,噪声低,振动小。
该系列离心机广泛适用于免疫血液学实验室、检验室、研究室,可进行红细胞血清学实验,做抗原、抗体的鉴定及库姆式实验的结果判断等,是各类医院血库,实验室,血站,医学院校和医学研究机构的必备设备。
医用细胞涂片离心机http://www.fameinstrument.cn/
自动脱帽离心机的工作原理是什么?自动脱帽离心机的使用守则是什么?该产品具有运行平稳,体积小、噪音低、造型美观、温升小,配有安全门锁装置,自动调节平衡,整机采用微电脑控制系统的一系列性能特征,自动脱帽离心机的工作原理是什么?自动脱帽离心机的使用守则是什么大家也明白吗?那迈克尔离心机小编给大家做个解释。
工作原理
自动脱帽离心机亦称脱盖离心机、真空采血管专用脱帽离心机、脱帽离心机。是指在离心的过程中,利用离心力,完成脱帽,集自动脱帽、离心一次完成,彻底解决医院在分离真空采血管中脱帽的难题,提高了工作效率。
使用守则
一.脱帽离心机在离心结束后取出转头要倒置于实验台上,擦干腔内余水,离心机盖处于打开状态。
二.转头盖摆放在离心机的平台上,或摆放在实验台上,千万不可不拧紧浮放在转头上,因为一旦误启动,转头盖就会飞出,造成事故!
三.转头盖在拧紧后一定要用手指触摸转头与转盖之间有无缝隙,如有缝隙要拧开重新拧紧,直至确认无缝隙方可启动离心机。
四.在离心过程中,操作人员不得离开离心机室,一旦发生异常情况操作人员不能关电源(POWER),要按STOP
五.不得使用伪劣的离心使管,不得用老化、变形、有裂纹、质量不合格的离心管。
六.在节假日和晚间最后一个使用离心机的工作人员必须例行安全检查后方能离去。
七.在仪器使用过程中发生机器故障,部件损坏情况时要及时与厂家。
自动脱帽离心机的工作原理是什么?自动脱帽离心机的使用守则是什么迈克尔离心机小编介绍了这么多,希望可以帮到大家。谢谢关注。
更多资讯请咨询离心机http://www.mekeerlxj.com/
本人和同学是刚刚参与临床试验的小白,主要参与样品处理部分。
实验室离心机用的是赛默飞的HeraeusMultifugeX1R。
测转速的仪器为testo470-精密型光学/机械转速测量仪,在离心机中心轴的位置贴上反射贴,测量时将仪器红色光速对准反射贴就可以测量。
然后,4月初,老板说想要自己做温度校准。
发现实验室自己做温度校准非常少。方法也都写的不全,咨询了离心机厂家,学习到了一些:
1.使用离心机前应该提前做预冷,2000rpm,转30min。
2.低温离心机的设定温度如4℃,是腔体温度,所以当离心机屏幕显示温度降到4℃时其中的离心样品不一定是4℃。
有两种自己可以做的温度校准。
1:用连有探头的热敏电阻温度计测量腔体温度。预冷后,将温度计探头放入离心机内盖上盖子测量温度即可。但是离心机内不同位置温度稍有不同,若探头贴在金属壁上温度会过低。另外,一定不能在离心机运转时测量!
2:测量样品温度。用甘油代替样品,配平后,2000rpm,转2h。没错,是两小时,甘油降温非常慢。之后,拿出离心管用快速插入式温度计测量甘油温度。我们购买了testo905-T1-刺入式温度计,还在试用中。(如果不用甘油而用水的话,拿出来测到的温度在4.6~5.8摄氏度左右。)
因为2h实在是太久了,实验室正好有5℃左右的冰箱,准备周四的时候试一试先把甘油放在冰箱,再放入预冷后的离心机离心一段时间,看看能减少多久时间。
如果有更好的方法请各位大大指导。

