- Overview
- Data/Specifications
- Literature/Support
- How It Works
Overview
IgE is the least abundant isotype but has the capability of triggering powerful immune responses by binding to Fc receptors on the surface of cells such as mast cells, basophils, eosinophils, monocytes, macrophages and platelets.
IgE is well known for its involvement in eliciting an allergic or asthmatic response.Ovalbumin (OVA) is widely used for inducing an allergic response in mouse models of allergic asthma. Through T and B cell signalling, IgE production is stimulated and released into the bloodstream where it attaches to receptors on cells such as mast cells and basophils. These cells are then activated upon cross-linking of the IgE with the antigen initiating the allergic cascade.
Data/Specifications
Species:mouse
Sample Type :cell culture supernate, serum
Sample Preparation:dilute 1:10, adjust as needed
Test Volume:100 uL
Length:2 hours 15 min
Range:7.8-500ng/mL
Sensitivity:< 3.8 ng/mL
Literature/Support
Product Insert:
Mouse OVA-IgE ELISA Insert (PDF, 276 KB)
Articles/Troublshooting:
ELISA Troubleshooting Guide
ELISA Data Reduction Guide
IgE mediated immune response in allergic asthma (blog post)
Youngblood, B. A., Brock, E. C., Leung, J., Falahati, R., Bochner, B. S., Rasmussen, H. S., ... & Tomasevic, N. (2019). Siglec-8 antibody reduces eosinophils and mast cells in a transgenic mouse model of eosinophilic gastroenteritis. JCI insight, 4(19).
Seo, M. Y., Kim, K. R., Lee, J. J., Ryu, G., Lee, S. H., Hong, S. D., ... & Kim, H. Y. (2019). Therapeutic effect of topical administration of red onion extract in a murine model of allergic rhinitis.Scientific reports,9(1), 2883.
Aliu, H., Rask, C., Brimnes, J., & Andresen, T. L. (2017). Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen.International journal of nanomedicine,12, 8377.
Sjöberg, L. C., Nilsson, A. Z., Lei, Y., Gregory, J. A., Adner, M., & Nilsson, G. P. (2017). Interleukin 33 exacerbates antigen driven airway hyperresponsiveness, inflammation and remodeling in a mouse model of asthma.Scientific reports,7(1), 1-10.
Kuo, C. H., Collins, A. M., Boettner, D. R., Yang, Y., & Ono, S. J. (2017). Role of CCL7 in type I hypersensitivity reactions in murine experimental allergic conjunctivitis.The Journal of Immunology,198(2), 645-656.
Liu, B., Lee, J. B., Chen, C. Y., Hershey, G. K. K., & Wang, Y. H. (2015). Collaborative Interactions between Type 2 Innate Lymphoid Cells and Antigen-Specific CD4+ Th2 Cells Exacerbate Murine Allergic Airway Diseases with Prominent Eosinophilia. The Journal of Immunology, 194(8), 3583-3593.
Aven, L., Paez-Cortez, J., Achey, R., Krishnan, R., Ram-Mohan, S., Cruikshank, W. W., & Ai, X. (2014). An NT4/TrkB-dependent increase in innervation links early-life allergen exposure to persistent airway hyperreactivity. The FASEB Journal, 28(2), 897-907.
Arakawa, T., Deguchi, T., Sakazaki, F., Ogino, H., Okuno, T., & Ueno, H. (2013). Supplementary seleno-L-methionine suppresses active cutaneous anaphylaxis reaction. Biological and Pharmaceutical Bulletin, 36(12), 1969-1974.
Reddy, A. T., Lakshmi, S. P., Dornadula, S., Pinni, S., Rampa, D. R., & Reddy, R. C. (2013). The nitrated fatty acid 10-nitro-oleate attenuates allergic airway disease. The Journal of Immunology, 191(5), 2053-2063.
Paez-Cortez, J., Krishnan, R., Arno, A., Aven, L., Ram-Mohan, S., Patel, K. R., & Fine, A. (2013). A new approach for the study of lung smooth muscle phenotypes and its application in a murine model of allergic airway inflammation.
Cloots, R. H., Sankaranarayanan, S., de Theije, C. C., Poynter, M. E., Terwindt, E., van Dijk, P., & Koehler, S. E. (2013). Ablation of Arg1 in hematopoietic cells improves respiratory function of lung parenchyma, but not that of larger airways or inflammation in asthmatic mice. American Journal of Physiology-Lung Cellular and Molecular Physiology, 305(5), L364-L376.
Knolle, M. D., Nakajima, T., Hergrueter, A., Gupta, K., Polverino, F., Craig, V. J., & Owen, C. A. (2013). Adam8 limits the development of allergic airway inflammation in mice. The Journal of Immunology, 190(12), 6434-6449.
Mays, L. E., Ammon-Treiber, S., Mothes, B., Alkhaled, M., Rottenberger, J., Müller-Hermelink, E. S., & Kormann, M. S. (2013). Modified Foxp3 mRNA protects against asthma through an IL-10–dependent mechanism. The Journal of clinical investigation, 123(3), 1216.
McKee, A. S., Burchill, M. A., Munks, M. W., Jin, L., Kappler, J. W., Friedman, R. S., & Marrack, P. (2013). Host DNA released in response to aluminum adjuvant enhances MHC class II-mediated antigen presentation and prolongs CD4 T-cell interactions with dendritic cells. Proceedings of the National Academy of Sciences, 110(12), E1122-E1131.
Asosingh, K., Cheng, G., Xu, W., Savasky, B. M., Aronica, M. A., Li, X., & Erzurum, S. C. (2013). Nascent endothelium initiates Th2 polarization of asthma. The Journal of Immunology, 190(7), 3458-3465.
Lombardi, V., Speak, A. O., Kerzerho, J., Szely, N., & Akbari, O. (2012). CD8α+ β− and CD8α+ β+ plasmacytoid dendritic cells induce Foxp3+ regulatory T cells and prevent the induction of airway hyper-reactivity. Mucosal immunology, 5(4), 432-443.
Nkyimbeng-Takwi, E. H., Shanks, K., Smith, E., Iyer, A., Lipsky, M. M., Detolla, L. J., & Chapoval, S. P. (2012). Neuroimmune semaphorin 4A downregulates the severity of allergic response. Mucosal immunology, 5(4), 409-419.
MacSharry, J., O"Mahony, C., Shalaby, K. H., Sheil, B., Karmouty-Quintana, H., Shanahan, F., & Martin, J. G. (2012). Immunomodulatory effects of feeding with Bifidobacterium longum on allergen-induced lung inflammation in the mouse. Pulmonary pharmacology & therapeutics, 25(4), 325-334.
Kim, S. R., Lee, K. S., Lee, K. B., & Lee, Y. C. (2012). Recombinant IGFBP‐3 inhibits allergic lung inflammation, VEGF production, and vascular leak in a mouse model of asthma. Allergy, 67(7), 869-877.
Emo, J., Meednu, N., Chapman, T. J., Rezaee, F., Balys, M., Randall, T., ... & Georas, S. N. (2012). Lpa2 is a negative regulator of both dendritic cell activation and murine models of allergic lung inflammation. The Journal of Immunology, 188(8), 3784-3790.
Diesner, S. C., Olivera, A., Dillahunt, S., Schultz, C., Watzlawek, T., Förster-Waldl, E., ... & Rivera, J. (2012). Sphingosine-kinase 1 and 2 contribute to oral sensitization and effector phase in a mouse model of food allergy. Immunology letters, 141(2), 210-219.
Carr, V. M., Robinson, A. M., & Kern, R. C. (2012). Tissue-specific effects of allergic rhinitis in mouse nasal epithelia. Chemical senses, bjs048.
Singh, S. P., Gundavarapu, S., Peña-Philippides, J. C., Mishra, N. C., Wilder, J. A., Langley, R. J., ... & Sopori, M. L. (2011). Prenatal secondhand cigarette smoke promotes Th2 polarization and impairs goblet cell differentiation and airway mucus formation. The Journal of Immunology, 187(9), 4542-4552.
Reisacher, W. R., Liotta, D., Yazdi, S., & Putnam, D. (2011, September). Desensitizing mice to ovalbumin through subcutaneous microsphere immunotherapy (SMITH). In International forum of allergy & rhinology (Vol. 1, No. 5, pp. 390-395). Wiley Subscription Services, Inc., A Wiley Company.
MacNeil, A. J., Yang, Y. J., & Lin, T. J. (2011). MAPK Kinase 3 Specifically Regulates FcεRI-Mediated IL-4 Production by Mast Cells. The Journal of Immunology, 187(6), 3374-3382.
Stemmy, E. J., Balsley, M. A., Jurjus, R. A., Damsker, J. M., Bukrinsky, M. I., & Constant, S. L. (2011). Blocking cyclophilins in the chronic phase of asthma reduces the persistence of leukocytes and disease reactivation. American journal of respiratory cell and molecular biology, 45(5), 991-998.
Kim, S. R., Lee, K. S., Park, S. J., Min, K. H., Lee, M. H., Lee, K. A., & Lee, Y. C. (2011). A novel dithiol amide CB3 attenuates allergic airway disease through negative regulation of p38 mitogen-activated protein kinase. American journal of respiratory and critical care medicine, 183(8), 1015-1024.
Wang, W., Zhu, Z., Zhu, B., & Ma, Z. (2011). Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis. Otolaryngology--Head and Neck Surgery, 144(4), 506-513.
Balsley, M. A., Malesevic, M., Stemmy, E. J., Gigley, J., Jurjus, R. A., Herzog, D., & Constant, S. L. (2010). A cell-impermeable cyclosporine A derivative reduces pathology in a mouse model of allergic lung inflammation. The Journal of Immunology, 185(12), 7663-7670.
Lauenstein, H. D., Quarcoo, D., Plappert, L., Schleh, C., Nassimi, M., Pilzner, C. & Groneberg, D. A. (2011). Pituitary adenylate cyclase‐activating peptide receptor 1 mediates anti‐inflammatory effects in allergic airway inflammation in mice. Clinical & Experimental Allergy, 41(4), 592-601.
Kim, S. R., Lee, K. S., Park, S. J., Min, K. H., Lee, M. H., Lee, K. A., ... & Lee, Y. C. (2011). A novel dithiol amide CB3 attenuates allergic airway disease through negative regulation of p38 mitogen-activated protein kinase. American journal of respiratory and critical care medicine, 183(8), 1015-1024.
Lyons, A., O"Mahony, D., O"Brien, F., MacSharry, J., Sheil, B., Ceddia, M., & O"Mahony, L. (2010). Bacterial strain‐specific induction of Foxp3+ T regulatory cells is protective in murine allergy models. Clinical & Experimental Allergy, 40(5), 811-819.
Wang, W., Zhu, Z., Zhu, B., & Ma, Z. (2011). Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis. Otolaryngology--Head and Neck Surgery, 144(4), 506-513.
Breslow, R. G., Rao, J. J., Xing, W., Hong, D. I., Barrett, N. A., & Katz, H. R. (2010). Inhibition of Th2 adaptive immune responses and pulmonary inflammation by leukocyte Ig-like receptor B4 on dendritic cells. The journal of immunology, 184(2), 1003-1013.
Yadav, U. C., Naura, A. S., Aguilera-Aguirre, L., Ramana, K. V., Boldogh, I., Sur, S., & Srivastava, S. K. (2009). Aldose reductase inhibition suppresses the expression of Th2 cytokines and airway inflammation in ovalbumin-induced asthma in mice. The Journal of Immunology, 183(7), 4723-4732.
Park, S. J., Lee, K. S., Kim, S. R., Min, K. H., Choe, Y. H., Moon, H., ... & Lee, Y. C. (2009). Peroxisome proliferator-activated receptor γ agonist down-regulates IL-17 expression in a murine model of allergic airway inflammation. The Journal of Immunology, 183(5), 3259-3267.
Larsen, S. T., Roursgaard, M., Jensen, K. A., & Nielsen, G. D. (2010). Nano titanium dioxide particles promote allergic sensitization and lung inflammation in mice. Basic & clinical pharmacology & toxicology, 106(2), 114-117.
Lee, C. G., Hartl, D., Lee, G. R., Koller, B., Matsuura, H., Da Silva, C. A., ... & Elias, J. A. (2009). Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13–induced tissue responses and apoptosis. The Journal of experimental medicine, 206(5), 1149-1166.
Beigelman, A., Gunsten, S., Mikols, C. L., Vidavsky, I., Cannon, C. L., Brody, S. L., & Walter, M. J. (2009). Azithromycin attenuates airway inflammation in a noninfectious mouse model of allergic asthma. CHEST Journal, 136(2), 498-506.
| References/Citations: | Application: |
| Tissue-specific effects of allergic rhinitis in mouse nasal epithelia Carr, V.M. et al., Chem Senses, Sep 2012; 37: 655 - 668. | Serum from individual animals was analyzed for the presence ofOVA-specific IgE using an ELISA kit purchased from MD Bioproducts. |
| Lpa2 is a negative regulator of both dendritic cell activation and murine models of allergic lung inflammation Emo, J et al., J. Immunol., Apr 2012; 188: 3784 - 3790 | Serum from individual animals was analyzed for the presence of OVA-specific IgE using an ELISA kit purchasedfrom MD Bioproducts. |
| Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis Wang, W. et al., Otolaryngology -- Head and Neck Surgery, Feb 2011 | Measure concentration of OVA-IgE from Balb/c mice with either PPAR-γ agonist pioglitazone (30 mg/kg/d) or pioglitazone plus PPAR-γ antagonist GW9662 (0.5 mg/d). |
| A Cell-Impermeable Cyclosporine A Derivative Reduces Pathology in a Mouse Model of Allergic Lung Inflammation Balsley, M et al., J. Immunol., Dec 2010; 185: 7663 | Serum from individual animals was analyzed for the presence of OVA-specific IgE using an ELISA kit purchasedfrom MD Bioproducts. |
| Inhibition of Th2 Adaptive Immune Responses and Pulmonary Inflammation by Leukocyte Ig-Like Receptor B4 on Dendritic Cells Breslow, R et al., J. Immunol., Jan 2010; 184: 1003 - 1013. | Measure the concentration of serum IgE in genetically modified female mice of the BALB/c background (6-12 weeks old). |
| A Novel Dithiol Amide CB3 attenuates allergic airway disease through negative regulation of p38 MAPKKim SR, et al. Am. J. Respir. Crit. Care Med. April 2010 | Serum from individual animals was analyzed for the presence ofOVA-specific IgE using an ELISA kit purchased from MD Bioproducts. |
| Aldose Reductase Inhibition Suppresses the Expression of Th2 Cytokines and Airway Inflammation in Ovalbumin-Induced Asthma in Mice Umesh C. S. Yadav et al.,J. Immunol., Oct 2009; 183: 4723 - 4732. | Measure the concentration of OVA-IgE in broncheoalveolar lavage (BAL) fluid from C57BL/6 mice. |
Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13–induced tissue responses and apoptosis Chun Geun Lee et al.,J. Exp. Med., May 2009; 206: 1149 - 1166. | Measure the concentration of OVA-IgE in splenocytes isolated from BALB/c WT and mutant mice. |
| Peroxisome Proliferator-Activated Receptor Agonist Down-Regulates IL-17 Expression in a Murine Model of Allergic Airway Inflammation Seoung Ju Park et al,J. Immunol., Sep 2009; 183: 3259 - 3267. | |
| Azithromycin Attenuates Airway Inflammation in a Noninfectious Mouse Model of Allergic Asthma Avraham Beigelman et al.,Chest, Aug 2009; 136: 498 - 506. | Measure the concentration of serum specific OVA-IgE in 7-week oldBALB/cJ female mice. |
How It Works
ELISA or Enzyme-linked Immunosorbent Assay is a colorimetric based immunoassay utilizing a capture antibodyand a detection antibody to provide a unique and powerful assay system. Antibody/antigen reactionstake place on the suface of microplate wells that have been previously coated with a monoclonal antibodyto mouse IgE heavy chain. Biotinylated ovalbumin and streptavidin-peroxidase, in the presence of substrate quantifies theanalyte bound.
Assay Principle:
ebiomall.com
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自动脱帽离心机的工作原理是什么?自动脱帽离心机的使用守则是什么?该产品具有运行平稳，体积小、噪音低、造型美观、温升小，配有安全门锁装置，自动调节平衡，整机采用微电脑控制系统的一系列性能特征，自动脱帽离心机的工作原理是什么?自动脱帽离心机的使用守则是什么大家也明白吗?那迈克尔离心机小编给大家做个解释。
工作原理
自动脱帽离心机亦称脱盖离心机、真空采血管专用脱帽离心机、脱帽离心机。是指在离心的过程中，利用离心力，完成脱帽，集自动脱帽、离心一次完成，彻底解决医院在分离真空采血管中脱帽的难题，提高了工作效率。
使用守则
一.脱帽离心机在离心结束后取出转头要倒置于实验台上，擦干腔内余水，离心机盖处于打开状态。
二.转头盖摆放在离心机的平台上，或摆放在实验台上，千万不可不拧紧浮放在转头上，因为一旦误启动，转头盖就会飞出，造成事故!
三.转头盖在拧紧后一定要用手指触摸转头与转盖之间有无缝隙，如有缝隙要拧开重新拧紧，直至确认无缝隙方可启动离心机。
四.在离心过程中，操作人员不得离开离心机室，一旦发生异常情况操作人员不能关电源(POWER)，要按STOP
五.不得使用伪劣的离心使管，不得用老化、变形、有裂纹、质量不合格的离心管。
六.在节假日和晚间最后一个使用离心机的工作人员必须例行安全检查后方能离去。
七.在仪器使用过程中发生机器故障，部件损坏情况时要及时与厂家。
自动脱帽离心机的工作原理是什么?自动脱帽离心机的使用守则是什么迈克尔离心机小编介绍了这么多，希望可以帮到大家。谢谢关注。
更多资讯请咨询离心机http://www.mekeerlxj.com/
RCF = 1.119 x 10-5 x (rpm)2 x r
其中r 表示离心机转轴中心与离心管中心的距离，单位为cm。由于离心管的位置由转子（rotor）决定，因此r 必须由查阅相关转子的参数而得。
普通生化检查分离血清，这个是用低速离心机转速在3000—3500转。一般在3分钟之内就可以了，分析分离效果，可以酌情调节转速和时间，用试验型的那种小的就行
至于管式离心机分离血清生产疫苗，这个有着严格的工艺规范，只能照做，不能随意更改参数
富一阳光与你交流离心机分离机设备相关问题，希望对你有帮助~
GB 19815-2005 离心机安全要求
GB 6065-1985 TZ立式振动离心机
GB 12258-1990 医用低速离心机
JB/T 3263-2000 卧式振动离心机
JB/T 4064-2005 上悬式离心机
JB/T 5519-1991高速冷冻离心机
JB/T 6118-1992 TCL沉降过滤离心机
JB/T 53190-1999 三足式离心机 产品质量分等
JB 447-1985卧式活塞推料离心机技术条件
JB/T 502-2004 螺旋卸料沉降离心机
JB/T 5284-1991 防爆型刮刀卸料离心机 序号 标 准 代 号 标 准 名 称 1 GB/T 4774-2004 分离机械 名词术语 2 GB 7779-2005 离心机型号编制方法 3 GB 10901-2005 离心机 性能测试方法 4 GB/19815-2005 离心机 安全要求 5 GB/T10894-2004 分离机械 噪音测试方法 6 GB/T10895-2004 离心机 分离机 机械振动测试方法 7 JB/T 447-2004 活塞推料离心机 8 JB/T 10411-2004 离心机、分离机奥氏体钢锻件超声检测及质量评级 9 JB/T 8051-2006 离心机转鼓强度计算规范 10 JB/T 8865-2001 活塞推料离心机用滤网 11 JB/T 9095-1999 离心机、分离机锻焊件无损探伤技术规范 12 JB/T 7217-2006 分离机械涂装通用技术条件 13 JB/T 6418-2006 分离机械 清洁度测定方法向左转|向右转
