Highlights
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready:Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased:Complete RNA recovery without miRNA loss.
Description
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
|---|---|
| Equipment | Microcentrifuge, vortex |
| Sample Inactivation | TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other infectious agents. |
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 50 µg RNA (binding capacity), ≥25 µl (elution volume) |
Q1: Is DNase I available for individual purchase?
All kit components are available for purchase separately.
Q2: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q3: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Q4: Is Direct-zol suitable for very small numbers of cells?
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q5: Is it possible to extract proteins with the Direct-zol RNA kits?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q6: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
Q10: Which phenol-based reagents are compatible with Direct-zol?
The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”
-Adina B. (University of Guelph)
“This kit is amazing, I"ve got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“
-R.K. CSU
“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”
-H.Z. (Harvard Medical School)
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| E1010-1-4 | DNA Digestion Buffer | 4 mL | $15.00 | |
| E1010-1-16 | DNA Digestion Buffer | 16 mL | $29.00 | |
| R2050-1-200 | TRI Reagent | 200 ml | $219.00 | |
| R2050-1-50 | TRI Reagent | 50 ml | $70.00 | |
| C1078-50 | Zymo-Spin IICR Columns | 50 Pack | $55.00 | |
| R1003-3-12 | RNA Wash Buffer | 12 ml | $30.00 | |
| R1003-3-48 | RNA Wash Buffer | 48 ml | $105.00 | |
| E1010 | DNase I Set | 250 U | $56.00 | |
| R2050-2-40 | Direct-zol RNA PreWash (Concentrate) | 40 ml | $42.00 | |
| R2050-2-160 | Direct-zol RNA PreWash (Concentrate) | 160 ml | $166.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| R2050 | Direct-zol RNA Miniprep | 50 preps | $180.00 | |
| R2052 | Direct-zol RNA Miniprep | 200 preps | $555.00 | |
| W1001-6 | DNase/RNase-Free Water | 6 ml | $15.00 | |
| W1001-30 | DNase/RNase-Free Water | 30 ml | $22.00 |
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纯水机,跟净水机或者净水器的区别是需要用电(大多数),有一个真空储水罐,纯水机包含了净水机或者净水器的功能和使用材质,过滤更高可达到万分之一微米,因为最重要的是它采用了RO反渗透技术,因为过滤更精细,所以一般的水压是不能顺畅通过的,所以需要用电,电要带动机器本身佩戴的增压泵来给原水打压才能通过。
事实上很多细菌病毒异色异味也只有纯水机能完全的过滤掉。
这只是一些最基本的常识。希望对楼主有帮助吧,本人专业从事净水行业多年,也可以看本人以往的知道回答!
总体上来说,在纯水仪器上,进口品牌中密理博、pall、威立雅都算是国内科研单位采购比较多的,当然,售价也较高,一般为国产品牌的3-5倍,除非硬性规定,或者需要用进口高端设备来提升实验室形象,否则,不建议花冤枉钱,因为目前国内很多纯水品牌在技术上都已经追赶甚至超越了进口品牌。
此外,很多人不了解的是,许多欧美品牌的纯水机,虽然技术成熟,但是都是基于国外的水质而研发,而我国自来水水质事实上比欧美国家的低(欧美国家的自来水可直接饮用),因为存在进口品牌的水机在我国水土不服的现象,甚至其前端处理仍需搭配国产设备,只是很多客户并不知晓,尤其是中央供水系统更是如此。这一点需要注意。
国产纯水品牌的话,鱼龙混杂,这里仅仅介绍几个知名度较高,市场做得比较大的,并简单分析对比:
1、锐思捷:在技术上比较成熟,水机的品质和进口品牌做过很多对比和验证,基本上也足以媲美进口品牌了,产品线包含小型机(10-60L)和大型中央供水系统(200L-5T),价格在中高位置;
2、和泰:主做小型机,中央供水系统较少,价格由近万至7万左右,小型机产品线丰富,在市场营销方面下的力度非常大;
3、力康:也算是中高端国产纯水仪器,同时也做其他实验室设备;
此外还有优普、易普易达等区域性品牌。
净水器使用砂滤、活性炭、超滤等技术,能在一定程度上净化水源。能去除泥沙、铁锈,但无法彻底去除水垢、细菌、病毒,属于初级过滤。使用KDF技术的净水器,能去除细菌、病毒,部分去除重金属,但无法去除水垢。
软水机基本都是采用离子交换技术,用钠型阳离子置换水中的钙镁离子(水碱),彻底去除水垢,可以给生活带来很多好处。
纯水机采用反渗透膜过滤技术,核心是微孔过滤,只有水分子和比水分子小的矿物质能通过膜并保留下来供饮用;细菌、病毒、水垢、污染物均不能通过膜,成为浓缩水排出;从而达到纯净水的标准。
我问弱了,请大家不要嘲笑我。
用DDBJ---350型便携式电导率仪测试,DJS-1C型铂黑电极,测的结果总是达不到要求,与纯水器上所显示的便高很多。
请问是测的方法不对或是所用的仪器不对?
你们所用的分析用水是怎么检测的?
多谢了!
急呀!

