Highlights
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready:Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased:Complete RNA recovery without miRNA loss.
Description
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
|---|---|
| Equipment | Microcentrifuge, vortex |
| Sample Inactivation | TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other infectious agents. |
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 50 µg RNA (binding capacity), ≥25 µl (elution volume) |
Q1: Is DNase I available for individual purchase?
All kit components are available for purchase separately.
Q2: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q3: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Q4: Is Direct-zol suitable for very small numbers of cells?
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q5: Is it possible to extract proteins with the Direct-zol RNA kits?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q6: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
Q10: Which phenol-based reagents are compatible with Direct-zol?
The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”
-Adina B. (University of Guelph)
“This kit is amazing, I"ve got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“
-R.K. CSU
“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”
-H.Z. (Harvard Medical School)
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| E1010-1-4 | DNA Digestion Buffer | 4 mL | $15.00 | |
| E1010-1-16 | DNA Digestion Buffer | 16 mL | $29.00 | |
| R2050-1-200 | TRI Reagent | 200 ml | $219.00 | |
| R2050-1-50 | TRI Reagent | 50 ml | $70.00 | |
| C1078-50 | Zymo-Spin IICR Columns | 50 Pack | $55.00 | |
| R1003-3-12 | RNA Wash Buffer | 12 ml | $30.00 | |
| R1003-3-48 | RNA Wash Buffer | 48 ml | $105.00 | |
| E1010 | DNase I Set | 250 U | $56.00 | |
| R2050-2-40 | Direct-zol RNA PreWash (Concentrate) | 40 ml | $42.00 | |
| R2050-2-160 | Direct-zol RNA PreWash (Concentrate) | 160 ml | $166.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| R2050 | Direct-zol RNA Miniprep | 50 preps | $180.00 | |
| R2052 | Direct-zol RNA Miniprep | 200 preps | $555.00 | |
| W1001-6 | DNase/RNase-Free Water | 6 ml | $15.00 | |
| W1001-30 | DNase/RNase-Free Water | 30 ml | $22.00 |
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没有采用RO反向渗透膜的水处理设备，基本上都可称为净水器。根据处理方法不同，有不同的叫法，如软水器、净水器等等。
有些同行用娃哈哈，能满足药典吗？纯水机呢？
用DDBJ---350型便携式电导率仪测试，DJS-1C型铂黑电极，测的结果总是达不到要求，与纯水器上所显示的便高很多。
请问是测的方法不对或是所用的仪器不对？
你们所用的分析用水是怎么检测的？
多谢了！
急呀！
1、机器本身故障
如果纯水机本身质量不好，对净水效果和出水量有直接影响，使用劣质纯水机水质没保障。问题一大堆。
解决办法：前期选购方面需要慎重。可以参考《纯水机选购注意事项》
2、水管漏水或堵塞
水管是运输水的唯一渠道，如果水管发生堵塞或漏水现象，对纯水机的出水量有直接影响。
解决办法：将水管漏水或者堵塞的地方剪切掉，如果堵塞或漏水范围太大，只能更换一条新的水管。
3、纯水机内部零件损坏
纯水机通过内部零件共同运作来净水，如果内部零件损坏，短期内虽然不会影响到产品的使用，但是会发生出水量降低的现象。长期不维修会引起产水二次污染。
解决办法：纯水机内部配件很多都是塑料组装，如果损坏了直接购买新的配置组装上去即可。不过要注意，在维修的时候要关闭进水球阀。
4、RO膜、反渗透膜杂质堆积
RO膜，反渗透膜是纯水机的灵魂，它们和出水量，净水效果等有直接关联，同时这种膜也是最容易发生问题的。纯水机出水少或者水质效果不好了很可能就是RO膜堆积杂质多了的原因。
解决办法：机器在使用一段时间之后（大概3-4个月）就对RO膜、反渗透膜进行清洗，确保水质中的污染物不会长时间堆积，这样是提升机器出水量，提升水质的最好办法。
我问弱了，请大家不要嘲笑我。
纯水机，跟净水机或者净水器的区别是需要用电(大多数)，有一个真空储水罐，纯水机包含了净水机或者净水器的功能和使用材质，过滤更高可达到万分之一微米，因为最重要的是它采用了RO反渗透技术，因为过滤更精细，所以一般的水压是不能顺畅通过的，所以需要用电，电要带动机器本身佩戴的增压泵来给原水打压才能通过。
事实上很多细菌病毒异色异味也只有纯水机能完全的过滤掉。
这只是一些最基本的常识。希望对楼主有帮助吧，本人专业从事净水行业多年，也可以看本人以往的知道回答！
