![SMOBIO/[QP5510] Q-PAGE™ TGN Precast Gel (Midi, 12 wells, 4-15%), 10 gels/Midi, 12 wells, 4-15%), 10 gels</span>
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Description
Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.
Q-PAGE™ TGN Precast Gels are available in gradient (4 to 15%) and fixed (10%) concentrations of polyacrylamide in 12- and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP4XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP5XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
User-friendly gel cassette:
Numbered and framed wells for sample loading
Labeled warning sign and green tape as reminder
Enhanced gel performance:
Enhanced gel electrophoresis speed
Better band separation
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.
Technical
Quick running, clear bands
Q-PAGE™ TGN Precast Gel can separate protein in 19 minutes using 300 V.
QP5510 Specifications
Gel | TGN(Tris-Glycine-Novel) | |
Buffersystems | Tris-Glycine (Laemmli) | |
Features | Quickrunning, clear bands | |
Cassettesize | Midi Gel (10 X 10 cm) | |
Geldimensions | 8.1 x 8.1 x0.1 cm (W x L xthickness) cm | |
Electrophoresissystem | Mini Gel Tank XCell SureLock, Hoefer SE260 | |
Well format& Capacity | 12 wells, 40 μl/well | |
Gelpercentage | 4-15 % | |
Accessorytray | Productiondescription Tip card Gel remover Cassetteopener | |
Manual
Manual_Q-PAGE™ TGN Precast Gel, Midi
SDS
SDS_Q-PAGE™ Precast Gel
Migration pattern
Setting Up and Running Q-PAGE™ Midi Precast Gel
Removing Q-PAGE Midi Gel from cassette
Setting up gel/membrane sandwich for Western transfer
Recommendations/Tips for Gel Running
1. Remove comb and tape before adaption. 2. Use fresh 1X running buffer for the inner cathode chamber. 3. Rinse the wells before sample loading. 4. Try 200 V first, and optimize the voltage and running time if needed. Do not set voltage lower than 100 V.
Sample Preparation for SDS-PAGE
1. Mix protein sample with 2X sample buffer.
2. Heat the diluted samples at 95°C for 5 min or at 70°C for 10 min.
3. Cool the diluted samples to 4°C and spin down the water condensed on tube surface. (If there is high viscosity part at bottom of tube, transfer supernatant to a new tube.)
Prepare Q-PAGE™ for Sample Loading
1.Open the blister tray of Q-PAGE™ Precast Gel.
2.Briefly rinse the gel cassette with ddH2O.
3.Remove tape and comb; avoid squeezing the gel.
4.Adapt Q-PAGE™ to electrophoresis system; instruction are provided below. (Invitrogen® Mini Gel Tank is recommended.)
5.Use a pipette to gently wash the wells with running buffer to remove residual storage buffer.
6.Fill the wells with running buffer prior to sample loading.
7.Load samples and pre-stained protein marker into numbered wells.
8.Fill both inner and outer chambers with running buffer to the highest level. Ensure gel wells are completely covered.
Power Setting for Running Q-PAGE™
Optimize the voltage and running time if needed.
| 150 V | 200 V*2 | 250 V*3 | 300 V*3 |
Running Time*1 | 50-70 mins | 35-55 mins | 25-40 mins | 15-30 mins |
Expected Current Initial (per gel) Final (per gel) |
35-45 mA 10-20 mA |
45-55 mA 20-25 mA |
75-85 mA 40-45 mA |
100-110 mA 60-70 mA |
Expected temperature | 25-30°C | 25-30 °C | 25-35°C | 30-40°C |
*1 Set voltage higher than 100 V is recommended.
*2 Try 200 V first, and optimize the voltage and running time if needed
*3 For higher voltage conditions, please use fresh running buffer for inner and outer chambers
*4 Running time varies depending on gel percentage, running buffer, temperature, and power supply.
Remove Q-PAGE™ Gel from Cassette
Open cassette immediately after electrophoresis. Avoid gel drying.
1.Insert the cassette opener into corners of cassette.
2.Sequentially pry the opener to separate the two plates.
3.Gently pull up notched plate and let gel stay on the front plate.
4.Use cassette opener to push through the slot in the cassette.
5.Carefully detach the gel from the bottom of gel.
- Avoid diagonally peeling the gel from the corner.
- If necessary, cut well separators with gel remover.
6.Gently remove the gel for further staining or Western blotting.
Gel Staining
Proteins separated using Q-PAGE™ Precast Gels can be further stained with most popular staining reagents, such as Coomassie dyes (R-250 or G-250), Silver-stain solution,
and FluoroStain™ Protein Fluorescent Staining Dye. (Cat. No. PS1000)
Transferring Protein from Q-PAGE™ to Blotting Membrane
1. After protein separation using Q-PAGE™, gently detach QPAGE™ from cassette and then equilibrate the gel in transfer buffer.
2. Pre-soak blotting membrane and filter papers in transfer buffer.
*Activate PVDF membrane in methanol before soaking in transfer buffer.
**Prepare 6 filter papers for one gel/membrane sandwich.
3. Assemble transfer sandwich by orientating cathode, sponge, filter papers, gel, membrane, filter papers, sponge, and anode. The protein goes to the direction of cathode to anode.
4. Carefully move roller over the gel/membrane to remove air bubbles and excess buffer until complete contact is established.
5. Insert transfer cassette into transfer module. Notice that black side of cassette should be next to black side of module.
6. Fill transfer tank with pre-cooled transfer buffer to the highest water level.
7. Set constant voltage at 100 V. Transfer for 90 minutes at low temperature condition. Pre-stained protein marker should be visible on the membrane after transfer is completed.
Transfer of proteins to the membrane can be checked using Ponceau S staining before blocking step.
Supplemental Information for Using Q-PAGE™ Precast Gel
Adapting Q-PAGE™ Midi Precast Gels to Invitrogen Mini Gel Tank Electrophoresis System
1. Place the Q-PAGE Midi Precast Gels with notched plate facing toward yourself. No extra adapter is needed.
2. Seat the gels on the bottom of Mini Gel Tank and close the cassette clamp.
3. Fill chambers with running buffer to the level of the fill line. Ensure gel wells are completely covered.
Adapting Q-PAGE™ Midi Precast Gels to other electrophoresis system, please follow the manufacturer’s instruction.
Buffer recipes
2X sample buffer with reducing agent
62.5 mM Tris-HCl pH 6.8, 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)
10X Tris-Glycine running buffer
30.0 g Tris base, 144.0 g Glycine, 10.0 g SDS. Bring up the volume to 1 L with ddH2O.
1X running buffer
Dilute 100 ml 10X running buffer with 900 ml ddH2O.
10X transfer buffer
30.0 g Tris base, 144.0 g Glycine. Bring up the volume to 1 L with ddH2O.
1X transfer buffer
*Cool 1X transfer buffer to 4°C before using.
Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O.
**Add SDS to 0.1% to promote transfer of high molecular weight proteins.
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Q-PAGE™ Precast Gel
Gel Type | Bis-Tris | TGN (Tris-Glycine-Novel) | ||||||
Buffer systems | MOPS and MES | Tris-Glycine (Laemmli) | ||||||
Features | Clear and sharp bands, high resolution | Quick running, clear bands | ||||||
Cassette size | Mini Gel(10 x 8.3 cm) | Midi Gel(10 X 10 cm) | Mini Gel(10 x 8.3 cm) | Midi Gel(10 X 10 cm) | ||||
Electrophoresis system | Bio-Rad systems | Mini Gel Tank Xcell SureLock, Hoefer SE260 | Bio-Rad systems | Mini Gel Tank Xcell SureLock, Hoefer SE260 | ||||
Well format & Capacity | 12 wells, 25 μl/well | 15 wells, 22 μl/well | 12 wells, 40 μl/well | 15 wells, 28 μl/well | 12 wells, 25 μl/well | 15 wells, 22 μl/well | 12 wells, 40 μl/well | 15 wells,28 μl/well |
Gel percentage/ Cat. No. | 8% | 8% | 8% | 8% | 10% | 10% | 10% | 10% |
QP2110 | QP2120 | QP3110 | QP3120 | QP4210 | QP4220 | QP5210 | QP5220 | |
12% | 12% | 12% | 12% | 4-15% | 4-15% | 4-15% | 4-15% | |
QP2310 | QP2320 | QP3310 | QP3320 | QP4510 | QP4520 | QP5510 | QP5520 | |
4-12% | 4-12% | 4-12% | 4-12% |
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QP2510 | QP2520 | QP3510 | QP3520 |
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ExcelBand™ Protein Markers
Ready-to-use— premixed with a loading buffer for direct loading, no need to boil
Broad range— 310 kDa to 5 kDa
Pre-stained bands — for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Enhanced bands— for quick reference
YesBlot™ Western Marker I
Ready-to-use — no need of mixing or heating before sample loading
Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots
Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Wide range — 10 clear bands from 15 to 200 kDa for size estimation
Quick reference — two enhanced bands (30 and 80 kDa)
FluoroStain™ Protein Fluorescent Staining Dye
Compatible to MASS analysis — compatible to the analysis of mass spectra, such as LC-MS/MS, MALDI-TOF, and etc.
High sensitivity — detection level achieve ~3 ng, similar to silver staining
Substitution of the Coomassie Blue protein staining method
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没有采用RO反向渗透膜的水处理设备，基本上都可称为净水器。根据处理方法不同，有不同的叫法，如软水器、净水器等等。
有些同行用娃哈哈，能满足药典吗？纯水机呢？
用DDBJ---350型便携式电导率仪测试，DJS-1C型铂黑电极，测的结果总是达不到要求，与纯水器上所显示的便高很多。
请问是测的方法不对或是所用的仪器不对？
你们所用的分析用水是怎么检测的？
多谢了！
急呀！
1、机器本身故障
如果纯水机本身质量不好，对净水效果和出水量有直接影响，使用劣质纯水机水质没保障。问题一大堆。
解决办法：前期选购方面需要慎重。可以参考《纯水机选购注意事项》
2、水管漏水或堵塞
水管是运输水的唯一渠道，如果水管发生堵塞或漏水现象，对纯水机的出水量有直接影响。
解决办法：将水管漏水或者堵塞的地方剪切掉，如果堵塞或漏水范围太大，只能更换一条新的水管。
3、纯水机内部零件损坏
纯水机通过内部零件共同运作来净水，如果内部零件损坏，短期内虽然不会影响到产品的使用，但是会发生出水量降低的现象。长期不维修会引起产水二次污染。
解决办法：纯水机内部配件很多都是塑料组装，如果损坏了直接购买新的配置组装上去即可。不过要注意，在维修的时候要关闭进水球阀。
4、RO膜、反渗透膜杂质堆积
RO膜，反渗透膜是纯水机的灵魂，它们和出水量，净水效果等有直接关联，同时这种膜也是最容易发生问题的。纯水机出水少或者水质效果不好了很可能就是RO膜堆积杂质多了的原因。
解决办法：机器在使用一段时间之后（大概3-4个月）就对RO膜、反渗透膜进行清洗，确保水质中的污染物不会长时间堆积，这样是提升机器出水量，提升水质的最好办法。
我问弱了，请大家不要嘲笑我。
纯水机，跟净水机或者净水器的区别是需要用电(大多数)，有一个真空储水罐，纯水机包含了净水机或者净水器的功能和使用材质，过滤更高可达到万分之一微米，因为最重要的是它采用了RO反渗透技术，因为过滤更精细，所以一般的水压是不能顺畅通过的，所以需要用电，电要带动机器本身佩戴的增压泵来给原水打压才能通过。
事实上很多细菌病毒异色异味也只有纯水机能完全的过滤掉。
这只是一些最基本的常识。希望对楼主有帮助吧，本人专业从事净水行业多年，也可以看本人以往的知道回答！
