Product Specifications:
Item# 1005 : Recombinant p17 HIV-1 Core Protein
Concentration: See vial
Mass/vial: See vial
Volume/vial: 1ml
Diluent: MES Buffer, pH. 6.5
Purity: >95%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen Liquid
Stability: At least 24 months at -75°C.
Application: ELISA, Western ELISA, Diagnostics
Description: Recombinant HIV-1 p17 is expressed as a Glutathione S-transferase (GST) fusion protien produced in the E.coli expression system.
Purification: This protein is purified by GST-agarose affinity chromotography and p17 is cleaved from the fusion protein by using Factor Xa. GST is removed from the mixture by a second passage through GST-agarose.
Specificity: This protein binds to HIV-1 positive human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: Recombinant p17 core protein contains authentic N and C termini which is normally processed from the sequence of the matrix antigen with relevant HIV-1 protease.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute p17 stock solution in MES buffer immediately before use. ELISA assays may be performed with p17 in solid phase in 10-100ng range. Western ELISA may be performed in 100-500ng depending on the specificity of the primary antibody used in the test system.
Glossary
Gene and Gene Products
Structural Proteins: Structural proteins – the products of gag, pol and env genes, which are essential components of the retroviral particle.
Regulatory Proteins: Regulatory proteins – tat and rev proteins of HIV/SIV and tax and rex proteins of HTLVs; essential for viral expression in infected cells.
Accessory Proteins: Accessory proteins – additional (non-regulatory) virion – and non virion-associated proteins produced by HIV/SIV retroviruses: vif, vpr, vpu, vpx, and nef. Although, the accessory proteins are not necessary for viral propagation in tissue culture, they have been conserved in the different isolates; this conservation and experimental observations suggest that their role in vivo is very important.
gag
gag – group-sepecifc antigens or capsid proteins; the precursor is the p55 myristoylated protein, which is processed to p17 (Matrix) p24 (Capsid) and p7 (NucleoCapsid) proteins by the viral protease. Other small proteins are generated from the gag polyprotein.
pol
pol – (p66) generates the viral enzymes protease (p11), reverse transcriptase (p51), endonuclease and integrase (p32) after the processing of a gag-pol precursor polyprotein by the viral protease; gag-pol precursor is produced by ribosome frameshifting.
env
env – viral glycoproteins produced as a precursor (gp160) and processed to the external glycoprotein (gp120) and the transmembrane glycoprotein (gp41). The mature proteins are held together by noncovalent interactions; as a result substantial amount of gp120 is released extracellularly. The external glycoprotein (gp120) contains the binding site for the CD4 receptor.
tat
tat – transactivator of HIV gene expression; one of the two necessary viral regulatory factors (tat and rev) for HIV gene expression. Two forms are known, tat-1 exon (minor form) of 72 amino acids, and tat-2 exon (major form) of 86 amino acids. The electrophoretic mobility of these two forms in SDS gels is anomalous; they are approximately 16 kD and 14 kD in weight. Low levels of both proteins are found in persistently infected cells. tat is localized primarily in the nucleolus/nucleus; it acts by binding to the TAR RNA element and activating transcription from the LTR promoter. Post-transcriptional effects of tat have been postulated.
rev
rev – the second necessary regulatory factor for HIV expression. A 19 kD phosphoprotein localized primarily in the nucleolus/nucleus, rev acts by binding to RRE and promoting the nuclear export, stabilization and utilization of the viral mRNAs containing RRE.
vif
vif – viral infectivity factor, typically 23 kD; required for the efficient transmission of cell-free virus in tissue culture. In the absence of vif, the produced viral particles are defective, while the cell-to-cell transmission of virus is not affected significantly. It has been reported that the cellular localization is in the Golgi (vif is not found in the virion).
nef
nef – approximately 27 kD non-virion protein found in the cytoplasm of infected cells. Potentially myristoylated and associated with the inner plasma membrane. One of the first HIV proteins to be produced in the infected cells, it is the most immunogenic of the accessory proteins and may be used in the future for diagnosis and staging of the disease. NEF is dispensable and probably suffers counter-selection during ex vivo viral propagation in vivo. Recent evidence suggests that SIV nef is required for viral propagation in vivo.
vpr
vpr – virion-associated protein of unknown function found in HIV-1, HIV-2, SIVmac, and SIVmnd; typically 15 kD. May be homologous to vpx. Also called “rap” for rapid.
vpu
vpu – protein that promotes extracellular release of viral particles. Found only in HIV-1. Integral membrane phosphoprotein of 16kd; similar to M2 protein of influenza virus. It may be involved in env maturation. It is not found in the virion.
vpx
vpx – virion protein of 12 kD found only in HIV-2 infection. (vpx may have some homology with vpr).
Related research paper:
ebiomall.com
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请回答明确,谢谢
装置是这样的:旋转蒸发仪上的冷凝管的水直接流到真空泵里以冷却真空泵然后再从真空泵里流出来.
现在的问题是,我第一天清洗过真空泵之后,第二天再用就发现真空泵里的水已经有股臭味了,有没有什么好的办法可以让它不臭?这样每天都处理的话,太浪费时间了.如果哪位有什么好主意的话,请告知,谢谢!
要看清楚每个真空泵的真空度，选择使用。有必要时，自己测定一下其真空度，若与所写数值有差别时，请告知管理员。
使用时记得加冷阱，有必要时请对所蒸产品进行预处理。
使用结束后，先停止加入，并等体系冷却至室温，再放气，关泵，撤掉冷阱，千万不要弄反顺序。
如发现真空泵管道漏气时，请及时与管理员联系。
若是由于个人操作失误，导致真空泵损坏时，管理员有权停其使用权限，并让其负责维修。
本规定自贴出起生效，请大家遵守以上规定。
