Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 111068 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
| AAV5 | 111068-AAV5 | Virus (100 µL at titer ≥ 4×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV-hSyn(Search Vector Database)
- Backbone sizew/o insert(bp)4579
- Total vector size (bp)6622
- Vector typeAAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature30°C
- Growth Strain(s)NEB Stable
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert namedLight1.2
- SpeciesSynthetic
- Insert Size (bp)2043
- GenBank IDMH244550
- Tag/ Fusion Protein
- Flag tag (N terminal on insert)
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ cloning siteBaMHI(not destroyed)
- 3′ cloning siteHindIII(not destroyed)
- 5′ sequencing primerccggccaccttggtcgcgtcc
- 3′ sequencing primerggagaaaatgaaagccatacgggaagc (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- pAAV-hSyn-dLight1.2.gb
- Addgene Notes
- Addgene Diagnostic Digest
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
- Article Citing this Plasmid
- 1 Reference
Information for AAV5 (Catalog # 111068-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pAAV-hSyn-dLight1.2 (#111068). In addition to the viral particles, you will also receive purified pAAV-hSyn-dLight1.2 plasmid DNA.
Synapsin-driven expression of dLight1.2 dopamine sensor. This sensor has a slightly increased dynamic range and is better for 2P imaging in vivo. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 4×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Data submitted about 111068-AAV5 by requesting scientist(s):
- Data 1: Mouse, Brain parenchyma
- Data 2: Mouse, Brain parenchyma
ebiomall.com
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请回答明确,谢谢
装置是这样的:旋转蒸发仪上的冷凝管的水直接流到真空泵里以冷却真空泵然后再从真空泵里流出来.
现在的问题是,我第一天清洗过真空泵之后,第二天再用就发现真空泵里的水已经有股臭味了,有没有什么好的办法可以让它不臭?这样每天都处理的话,太浪费时间了.如果哪位有什么好主意的话,请告知,谢谢!
要看清楚每个真空泵的真空度,选择使用。有必要时,自己测定一下其真空度,若与所写数值有差别时,请告知管理员。
使用时记得加冷阱,有必要时请对所蒸产品进行预处理。
使用结束后,先停止加入,并等体系冷却至室温,再放气,关泵,撤掉冷阱,千万不要弄反顺序。
如发现真空泵管道漏气时,请及时与管理员联系。
若是由于个人操作失误,导致真空泵损坏时,管理员有权停其使用权限,并让其负责维修。
本规定自贴出起生效,请大家遵守以上规定。

