RecombinantHumanIL-4(CHO-expressed)ProteinSummary
DetailsofFunctionality | MeasuredinacellproliferationassayusingTF‑1humanerythroleukemiccells.Kitamura,T.etal.(1989)J.CellPhysiol.140:323.TheED50forthiseffectis0.05-0.2ng/mL. |
Source | ChineseHamsterOvarycellline,CHO-derivedhumanIL-4proteinHis25-Ser153 |
Accession# | P05112 |
N-terminalSequence | His25 |
Structure/Form | Monomer |
Protein/PeptideType | RecombinantProteins |
Gene | IL4 |
Purity | >95%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain |
EndotoxinNote | <0.01 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">0.01> |
Applications/Dilutions
TheoreticalMW | 15.0kDa.Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors. | |
SDS-PAGE | 17-25kDa,reducingconditions | |
Publications |
|
Packaging,Storage&Formulations
Storage | Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
|
Buffer | Lyophilizedfroma0.2μmfilteredsolutioninPBSwithBSAasacarrierprotein. |
Purity | >95%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain |
ReconstitutionInstructions | Reconstituteat100μg/mLinPBScontainingatleast0.1%humanorbovineserumalbumin. |
Notes
AlternateNamesforRecombinantHumanIL-4(CHO-expressed)Protein
- Bcellgrowthfactor1
- BCDF
- B-cellstimulatoryfactor1
- BCGF1
- BCGF-1
- binetrakin
- BSF1
- BSF-1
- IL4
- IL-4
- IL-4B_cellstimulatoryfactor1
- interleukin4
- interleukin-4
- Lymphocytestimulatoryfactor1
- MGC79402
- pitrakinra
Background
Interleukin-4(IL-4),alsoknownasBcell-stimulatoryfactor-1,isamonomeric,approximately13kDa‑18kDaTh2cytokinethatshowspleiotropiceffectsduringimmuneresponses(1‑3).Itisaglycosylatedpolypeptidethatcontainsthreeintrachaindisulfidebridgesandadoptsabundledfouralpha-helixstructure(4).HumanIL-4issynthesizedwitha24aasignalsequence.Alternatesplicinggeneratesanisoformwitha16aainternaldeletion.MaturehumanIL-4shares55%,39%and43%aasequenceidentitywithbovine,mouse,andratIL-4,respectively.Human,mouse,andratIL-4arespecies-specificintheiractivities(5-7).IL-4exertsitseffectsthroughtworeceptorcomplexes(8,9).ThetypeIreceptor,whichisexpressedonhematopoieticcells,isaheterodimeroftheligandbindingIL-4Ralphaandthecommongammachain(asharedsubunitofthereceptorsforIL-2,-7,-9,-15,and‑21).ThetypeIIreceptoronnonhematopoieticcellsconsistsofIL-4RalphaandIL-13Ralpha1.ThetypeIIreceptoralsotransducesIL-13mediatedsignals.IL-4isprimarilyexpressedbyTh2-biasedCD4+Tcells,mastcells,basophils,andeosinophils(1,2).Itpromotescellproliferation,survival,andimmunoglobulinclassswitchtoIgG4andIgEinhumanBcells,acquisitionoftheTh2phenotypebynaïveCD4+Tcells,primingandchemotaxisofmastcells,eosinophils,andbasophils,andtheproliferationandactivationofepithelialcells(10‑13).IL-4playsadominantroleinthedevelopmentofallergicinflammationandasthma(12,14).
- Benczik,M.andS.L.Gaffen(2004)Immunol.Invest.33:109.
- Chomarat,P.andJ.Banchereau(1998)Int.Rev.Immunol.17:1.
- Yokota,T.etal.(1986)Proc.Natl.Acad.Sci.83:5894.
- Redfield,C.etal.(1991)Biochemistry30:11029.
- Ramirez,F.etal.(1988)J.Immunol.Meth.221:141.
- Leitenberg,D.andT.L.Feldbush(1988)Cell.Immunol.111:451.
- Mosman,T.R.etal.(1987)J.Immunol.138:1813.
- Mueller,T.D.etal.(2002)Biochim.Biophys.Acta1592:237.
- Nelms,K.etal.(1999)Annu.Rev.Immunol.17:701.
- Paludan,S.R.(1998)Scand.J.Immunol.48:459.
- Corthay,A.(2006)Scand.J.Immunol.64:93.
- Ryan,J.J.etal.(2007)Crit.Rev.Immunol.27:15.
- Grone,A.(2002)Vet.Immunol.Immunopathol.88:1.
- Rosenberg,H.F.etal.(2007)J.AllergyClin.Immunol.119:1303.
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