As part of the Signal-Seeker™ product line, IgG control beads (CIG01) have been developed as highly specific negative control beads for Cytoskeleton"s mouse, monoclonal antibody-based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition. The IgG antibodies are covalently attached to minimize antibody heavy and light chain leaching, which makes interpretation of results easier. Additionally, these control beads are effective for pre-clearing lysates without risk of contamination with IgG antibody. IgG control beads (CIG01) are included in our comprehensive Signal-Seeker™ antibody based Detection Kits and utilizing these kits are recommend for first time PTM investigators.
Each lot of affinity-bead is quality controlled to provide high batch to batch consistency, see COA documents.
Application 1: Utilization of IgG control beads
IgG control beads are compatible with both the phosphotryosine and SUMOylation 2/3 affinity beads and Signal-Seeker kits. Note these beads do not have cross reactivity with either of these PTMs (Figure 1).
Figure 1: A431 cell were lysed with BlastR buffer. 1mg of protein was incubated with APY03-beads (pY) or CIG01 (IgG control). Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using a phosphotyrosine antibody (APY03) was performed. 1mg of protein was incubated with ASM24-beads (SUM0 2/3) or CIG01 (IgG control).Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using a SUMO 2/3 antibody (ASM23) was performed.
Amount:Each package contains enough IgG control beads for 10 reactions.
For more information contact: signalseeker@cytoskeleton.com
Associated Products:
Signal-Seeker™ Phosphotyrosine Detection Kit (Cat. # BK160)
Signal-Seeker™ SUMOylation 2/3 Detection Kit (Cat. # BK162)
Signal-Seeker™ Phosphotyrosine Affinity Beads (Cat.# APY03-beads)
Signal-Seeker™ SUMOylation 2/3 Affinity Beads (Cat.# ASM24-beads)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
Click on the pdf icon below to download the datasheet 
For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
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文章原文链接http://www.jbc.org/content/282/23/16776.long
IgM是免疫球蛋白M(Immunoglobulin M，IgM)的缩写。根据结构的不同将免疫球蛋白分为五种，IgM是人的免疫球蛋白之一，其他还有lgA、lgG、IgD和lgE。
问题分析：单纯疱疹是由单纯疱疹病毒感染引起的.单纯疱疹病毒最大的特点是,这种病毒很难被“斩尽杀绝”,总有一少部分病毒在体内潜伏下来,伺机作乱.这些病毒何时再次致病很难预料,一般情况下发热,情绪紧张,月经,妊娠等因素都可能引起发病或复发.发病后即使不经治疗,患者2--4周后也可自愈,只是部分病毒仍然潜伏在体内.目前对这种由单纯疱疹病毒引起的疾病,没有很好的药物治疗,发作时主要使用各种洛韦类抗病毒药,如阿昔洛韦等.主要还是要保持良好的情绪,避免劳累等.
指导建议：IGG和IGM是两种不同的免疫球蛋白，两者产生的先后顺序不一样，一般来说IGM阳性提示在急性感染期，IGG阳 性有可能与以前曾经有过感染有关的。aware可自测不用抽血祝您健康天 猫！
