As part of the Signal-Seeker™ product line, IgG control beads (CIG01) have been developed as highly specific negative control beads for Cytoskeleton"s mouse, monoclonal antibody-based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition. The IgG antibodies are covalently attached to minimize antibody heavy and light chain leaching, which makes interpretation of results easier. Additionally, these control beads are effective for pre-clearing lysates without risk of contamination with IgG antibody. IgG control beads (CIG01) are included in our comprehensive Signal-Seeker™ antibody based Detection Kits and utilizing these kits are recommend for first time PTM investigators.
Each lot of affinity-bead is quality controlled to provide high batch to batch consistency, see COA documents.
Application 1: Utilization of IgG control beads
IgG control beads are compatible with both the phosphotryosine and SUMOylation 2/3 affinity beads and Signal-Seeker kits. Note these beads do not have cross reactivity with either of these PTMs (Figure 1).
Figure 1: A431 cell were lysed with BlastR buffer. 1mg of protein was incubated with APY03-beads (pY) or CIG01 (IgG control). Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using a phosphotyrosine antibody (APY03) was performed. 1mg of protein was incubated with ASM24-beads (SUM0 2/3) or CIG01 (IgG control).Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using a SUMO 2/3 antibody (ASM23) was performed.
Amount:Each package contains enough IgG control beads for 10 reactions.
For more information contact: signalseeker@cytoskeleton.com
Associated Products:
Signal-Seeker™ Phosphotyrosine Detection Kit (Cat. # BK160)
Signal-Seeker™ SUMOylation 2/3 Detection Kit (Cat. # BK162)
Signal-Seeker™ Phosphotyrosine Affinity Beads (Cat.# APY03-beads)
Signal-Seeker™ SUMOylation 2/3 Affinity Beads (Cat.# ASM24-beads)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
Click on the pdf icon below to download the datasheet 
For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
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IgM是免疫球蛋白M(Immunoglobulin M，IgM)的缩写。根据结构的不同将免疫球蛋白分为五种，IgM是人的免疫球蛋白之一，其他还有lgA、lgG、IgD和lgE。
HSV1型导致的一般都是腰部以上的感染，典型症状是水泡有可能引起脑炎，而且有非活化状态的潜伏。所以我觉得你根本就不用担心这个抗体IgG阳性的化验单。
2-IgM也是一种特异性感染指标，但在人体内持续时间不长（有时是30天更短），往往是传染病的近期感染指标。呈阴性是指：你近期没有感染过结核杆菌。
文章原文链接http://www.jbc.org/content/282/23/16776.long
