The Matchmaker Mammalian Assay Kit 2 allows rapid and convenient analysis of protein-protein interactions in transfected mammalian cells. The kit uses a SEAP secreted reporter to measure the interactions between the two proteins of interest, eliminating the need for cell lysis.
Because these two-hybrid assays are performed in mammalian cells, proteins encoded by mammalian cDNAs are more likely to be in their native conformation; therefore, post-translational modifications and experimental results are more likely to represent biologically significant interactions.
The Matchmaker Mammalian Two-Hybrid Assay Kit 2 allows rapid and convenient analysis of protein-protein interactions in transfected mammalian cells. The kit uses a SEAP secreted reporter to measure the interactions between the two proteins of interest, eliminating the need for cell lysis.
Because these two-hybrid assays are performed in mammalian cells, proteins encoded by mammalian cDNAs are more likely to be in their native conformation; therefore, post-translational modifications and experimental results are more likely to represent biologically significant interactions.
The Matchmaker Mammalian Assay Kit 2 is based on the same principle as the Matchmaker Gold Two-Hybrid System—two fusion proteins are expressed that contain either a DNA binding domain or an activation domain. If the proteins interact, transcription of the SEAP reporter gene is activated from the pG5-SEAP plasmid.
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IgM是免疫球蛋白M(Immunoglobulin M，IgM)的缩写。根据结构的不同将免疫球蛋白分为五种，IgM是人的免疫球蛋白之一，其他还有lgA、lgG、IgD和lgE。
HSV1型导致的一般都是腰部以上的感染，典型症状是水泡有可能引起脑炎，而且有非活化状态的潜伏。所以我觉得你根本就不用担心这个抗体IgG阳性的化验单。
2-IgM也是一种特异性感染指标，但在人体内持续时间不长（有时是30天更短），往往是传染病的近期感染指标。呈阴性是指：你近期没有感染过结核杆菌。
文章原文链接http://www.jbc.org/content/282/23/16776.long
