- SynonymGALE,UDP-glucose 4-epimerase,Galactowaldenase
- SourceHuman Galactowaldenase, His Tag (GAE-H5142) is expressed from E.coli cells. It contains AA Ala 2 - Ala 348 (Accession # AAH01273).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 39.1 kDa. The protein migrates as 39 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human Galactowaldenase, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundUDP-glucose 4-epimerase, a member of the NAD(P)-dependent epimerase (dehydratase) family, is also known as galactowaldenase (GALE), UDP-N-acetylgalactosamine 4-epimerase (UDP-GalNAc 4-epimerase) and UDP-N-acetylglucosamine 4-epimerase (UDP-GlcNAc 4-epimerase),which is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. GALE can catalyze two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and GALE (UDP-GalNAc) when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.
- References
- (1)Schulz J.M., et al., 2004, J. Biol. Chem. 279:32796-32803.
- (2)Thoden J.B., et al, 2001, J. Biol. Chem. 276:15131-15136.
- (3)Holden HM., et al., 2003, J. Biol. Chem. 278 (45): 43885–8.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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IgM是免疫球蛋白M(Immunoglobulin M,IgM)的缩写。根据结构的不同将免疫球蛋白分为五种,IgM是人的免疫球蛋白之一,其他还有lgA、lgG、IgD和lgE。
HSV1型导致的一般都是腰部以上的感染,典型症状是水泡有可能引起脑炎,而且有非活化状态的潜伏。所以我觉得你根本就不用担心这个抗体IgG阳性的化验单。
2-IgM也是一种特异性感染指标,但在人体内持续时间不长(有时是30天更短),往往是传染病的近期感染指标。呈阴性是指:你近期没有感染过结核杆菌。
文章原文链接http://www.jbc.org/content/282/23/16776.long

