![ACROBiosystems/Human PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686) Protein, His Tag/100ug/PC9](images/ACROBiosystems/PC9-H5223.jpg)
- SynonymPCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
- SourceHuman PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His Tag (PC9-R52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 152 & Ser 154 - Ala 686 (Accession # Q8NBP7-1 (Human) & A0A5F9DJY7-1 (Rabbit)).Predicted N-terminus: Gln 31 & Ser 154Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non covalent interactions and with a calculated MW of 13.8 kDa and 58.0 kDa respectively. The protein migrates as 17 kDa and 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
![Human PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His Tag (Cat. No. PC9-R52H5) SDS-PAGE gel](images/ACROBiosystems/PC9-R52H5.jpg)
Human PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
![Human PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His TagHuman PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His Tag (Cat. No. PC9-R52H5) ELISA bioactivity](images/ACROBiosystems/PC9-R52H5-E1.jpg)
Immobilized Human LDL R, Fc Tag (Cat. No. LDR-H5254) at 2 μg/mL (100 μL/well) can bind Human PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His Tag (Cat. No. PC9-R52H5) with a linear range of 0.078-0.625 μg/mL (QC tested).
![Human PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His TagHuman PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His Tag (Cat. No. PC9-R52H5) ELISA bioactivity](images/ACROBiosystems/PC9-R52H5-E2.jpg)
Immobilized Human PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His Tag (Cat. No. PC9-R52H5) at 2 μg/mL (100 μL/well) can bind Rabbit LDL R, Fc Tag (Cat. No. LDR-R5255) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
- BackgroundProprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
- References
- (1)Seidah NG, et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100 (3): 928–33.
- (2)Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
- (3)Dubuc G. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (8): 1454–9.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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文章原文链接http://www.jbc.org/content/282/23/16776.long
您好,对于优生学检查,有两种抗体,一种是IGG抗体,一种是IGM抗体,如果是前者阳性,只能说明即往以前有感染了这类病原体,而不能说明当前正在感染这个病毒的。
指导意见:
如果是后一种阳性,也就是说IGM抗体阳性时,就提示当前正在感染这个病毒的,需要及时行抗病毒的药物治疗为佳,治好后才行怀孕的,否则对于怀孕的胎儿有很大的影响的。
aware可自测不用抽血祝您健康天 猫!
本试验根据《中国药典》2015年版紫外分光光度法吸收系数法进行制定,本试验方法采用的方法学验证内容如下:
1.线性及范围
取IgG标准品配制5个不同浓度被测样品,并进行测定,绘制标准曲线,得到回归方程及范围。
2.准确度
已回收率对准确度进行验证。以已知含量的IgG标准品配制供试品,配制三个浓度,每个浓度平均测定三次。计算回收率。
3.精密度
IgG标准品配制供试品,平行测定六次。
4.溶液稳定性试验
至少持续2h的溶液稳定性考察。
5.干扰试验
空白溶液中加入辅料,在280nm处测定吸收,确定辅料是否对样品的吸收造成干扰。
以上,请大神指正。
补充问题,在专属性验证上,仅做辅料干扰是不是太少了?是否再进行强降解实验呢?因为其中包含了蛋白质A、外源性DNA及宿主细胞蛋白残留。我是否应该针对这三种杂质进行专属性试验?该如何进行呢?
IgM是免疫球蛋白M(Immunoglobulin M,IgM)的缩写。根据结构的不同将免疫球蛋白分为五种,IgM是人的免疫球蛋白之一,其他还有lgA、lgG、IgD和lgE。
问题分析:单纯疱疹是由单纯疱疹病毒感染引起的.单纯疱疹病毒最大的特点是,这种病毒很难被“斩尽杀绝”,总有一少部分病毒在体内潜伏下来,伺机作乱.这些病毒何时再次致病很难预料,一般情况下发热,情绪紧张,月经,妊娠等因素都可能引起发病或复发.发病后即使不经治疗,患者2--4周后也可自愈,只是部分病毒仍然潜伏在体内.目前对这种由单纯疱疹病毒引起的疾病,没有很好的药物治疗,发作时主要使用各种洛韦类抗病毒药,如阿昔洛韦等.主要还是要保持良好的情绪,避免劳累等.
指导建议:IGG和IGM是两种不同的免疫球蛋白,两者产生的先后顺序不一样,一般来说IGM阳性提示在急性感染期,IGG阳 性有可能与以前曾经有过感染有关的。aware可自测不用抽血祝您健康天 猫!
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