Eukaryotic Oncostatin M (OSM)
OS-M
- Product No.EPA110Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourceEukaryotic expression
- Host293F cell
- Endotoxin Level<1.0EU per 1ug (determined by the LAL method)
- Subcellular LocationSecreted
- Predicted Molecular Mass23.5kDa
- Accurate Molecular Mass24kDa(Analysis of differences refer to the manual)
- Residues & TagsAla26~Arg220 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point9.4
- ApplicationsPositive Control; Immunogen; SDS-PAGE; WB.If bio-activity of the protein is needed, please check active protein.
- DownloadInstruction Manual
- UOM10µg50µg200µg1mg5mg
- FOBUS$ 256 For more details, please contact local distributors!US$ 640 For more details, please contact local distributors!US$ 1280 For more details, please contact local distributors!US$ 3840 For more details, please contact local distributors!US$ 9600 For more details, please contact local distributors!
SEQUENCE of the Eukaryotic Oncostatin M (OSM)

USAGE of the Eukaryotic Oncostatin M (OSM)
Reconstitute in PBS or others.
STORAGE of the Eukaryotic Oncostatin M (OSM)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Eukaryotic Oncostatin M (OSM)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
INCREMENT SERVICES
BCA Protein Quantification KitProtein Labeling Customized ServiceMolecular Mass Marker for ProteinMonoclonal Antibody Customized ServicePolyclonal Antibody Customized ServiceProtein Activity Test Experiment ServiceImmunoprecipitation (IP) Experiment ServiceElectrophoretic Mobility Shift Assay (EMSA) Experiment ServiceBufferLentivirus Packaging Experiment ServiceAdenovirus Packaging Experiment ServiceReal Time PCR Experimental ServiceSpike RBD Protein (S-RBD)Protein GProtein A
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| EPA110Hu61 | Eukaryotic Oncostatin M (OSM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA110Hu01 | Recombinant Oncostatin M (OSM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA110Hu01 | Active Oncostatin M (OSM) | Cell culture; Activity Assays. |
| PAA110Hu06 | Polyclonal Antibody to Oncostatin M (OSM) | WB; IHC; ICC; IP. |
| PAA110Hu01 | Polyclonal Antibody to Oncostatin M (OSM) | WB; IHC; ICC; IP. |
| MAA110Hu22 | Monoclonal Antibody to Oncostatin M (OSM) | WB; IHC; ICC; IP. |
| SEA110Hu | ELISA Kit for Oncostatin M (OSM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA110Hu | CLIA Kit for Oncostatin M (OSM) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA110Hu01 | ELISA Kit DIY Materials for Oncostatin M (OSM) | Main materials for "Do It (ELISA Kit) Yourself". |
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1,IgG抗体是抗体中分子量最小的一种,可通过胎盘输给胎儿,保护了婴儿最初六个月内免受感染。该抗体产生晚,维持时间长,消失慢,浓度高。血中检测到可作为远期感染指标。
2,IgM抗体是抗体中分子量最大的一种,不能通过胎盘输给胎儿。抗体产生最早,一经感染,快速产生,在感染初期抗感染起作用。但维持时间短,消失快。
3,灵长类动物主要有四种免疫球蛋白IgG、IgM、IgA、和IgE。
所有用于检测抗原的免疫学方法经适当改良后,均可用于抗体的检测,如IFA、ELISA、RIA、LA......由于抗原方法的敏感性提高和PCR技术的应用,使得HSV抗体在HSV感染个体中的不均一性和不稳定性影响了这类指标在临床诊断中的意义,但作为一种感染有关指标,在一定的范围和情况下,仍有必要进行检测和深入研究.目前HSV特异性抗体的检测,主要有IgG、IgM和IgA三种。
本试验根据《中国药典》2015年版紫外分光光度法吸收系数法进行制定,本试验方法采用的方法学验证内容如下:
1.线性及范围
取IgG标准品配制5个不同浓度被测样品,并进行测定,绘制标准曲线,得到回归方程及范围。
2.准确度
已回收率对准确度进行验证。以已知含量的IgG标准品配制供试品,配制三个浓度,每个浓度平均测定三次。计算回收率。
3.精密度
IgG标准品配制供试品,平行测定六次。
4.溶液稳定性试验
至少持续2h的溶液稳定性考察。
5.干扰试验
空白溶液中加入辅料,在280nm处测定吸收,确定辅料是否对样品的吸收造成干扰。
以上,请大神指正。
补充问题,在专属性验证上,仅做辅料干扰是不是太少了?是否再进行强降解实验呢?因为其中包含了蛋白质A、外源性DNA及宿主细胞蛋白残留。我是否应该针对这三种杂质进行专属性试验?该如何进行呢?
问题分析:单纯疱疹是由单纯疱疹病毒感染引起的.单纯疱疹病毒最大的特点是,这种病毒很难被“斩尽杀绝”,总有一少部分病毒在体内潜伏下来,伺机作乱.这些病毒何时再次致病很难预料,一般情况下发热,情绪紧张,月经,妊娠等因素都可能引起发病或复发.发病后即使不经治疗,患者2--4周后也可自愈,只是部分病毒仍然潜伏在体内.目前对这种由单纯疱疹病毒引起的疾病,没有很好的药物治疗,发作时主要使用各种洛韦类抗病毒药,如阿昔洛韦等.主要还是要保持良好的情绪,避免劳累等.
指导建议:IGG和IGM是两种不同的免疫球蛋白,两者产生的先后顺序不一样,一般来说IGM阳性提示在急性感染期,IGG阳 性有可能与以前曾经有过感染有关的。aware可自测不用抽血祝您健康天 猫!
2-IgM也是一种特异性感染指标,但在人体内持续时间不长(有时是30天更短),往往是传染病的近期感染指标。呈阴性是指:你近期没有感染过结核杆菌。
HSV1型导致的一般都是腰部以上的感染,典型症状是水泡有可能引起脑炎,而且有非活化状态的潜伏。所以我觉得你根本就不用担心这个抗体IgG阳性的化验单。


