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Description
Details
RACTIVITY | Human |
SENSITIVITY | <0.2 μg/mL |
ASSAY RANGE | 0.75-12 μg/mL |
REAGENTS PROVIDED | B2MGMICROTITER PLATEB2MGCONJUGATEB2MGSTANDARD – 12 μg/mLB2MGSTANDARD–6μg/mLB2MGSTANDARD–3 μg/mLB2MGSTANDARD–1.5 μg/mLB2MGSTANDARD–0.75 μg/mLB2MGSTANDARD–0 μg/mLSUBSTRATE ASUBSTRATE BSTOP SOLUTIONSAMPLE DILUENT |
INTENDED USE
This Human Beta2-Microglobulin ELISA Kit is to be used for in vitro quantitative determination of Beta2-Microglobulin (B2MG) concentrations in human serum, other biological samples, and cell culture supernatant. The kit is intended for RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
INTRODUCTION
Beta 2 microglobulin (B2MG) is initially discovered as a small serum protein of 12,000 Dalton. The small size allows B2MG to pass through the glomerular membrane, but it is normally completely reabsorbed in the proximal tubules. B2MG normally presents in minute amount, but increases its expressions, or adapts to the fibrillary configuration in certain pathological states.
B2MG is found to be a subunit of class I major histocompatibility complex (MHC I) that is expressed on all nucleated cell excluding red blood cells. B2MG non-covalently links with the a3 domain of human leukocyte antigen (HLA) to form MHC I. Inside cytoplasm, MHC I binds to intracellular degraded foreign peptides, or self-peptides in some conditions, through the a1 and a2 variable domains of HLA. Subsequently, MHC I is anchored to the cell surface through the a3 domain of HLA. By way of this procedure, MHC I presents the engulfed foreign peptides to (CD8+) cytotoxic T-cell, thus, plays an important role in cell-mediated immunity.
When binding with HFE protein, B2MG was found to increase iron uptake. B2MG is also associated with MHC I - like molecules such as CD1 and Qa. This immunoassay is a tool for investigation of the function and regulation of the protein.
PRINCIPLE OF THE ASSAY
This B2MG enzyme linked immunosorbent assay (ELISA) kit uses a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for B2MG. Standards or samples are added to the microtiter plate wells and B2MG, if present, will bind to the antibody pre-coated on the wells. A preparation of horseradish peroxidase (HRP)-conjugated B2MG antibody is added to each well. The conjugated antibody will bind to the B2MG immobilized on the plate after incubation. All unbound components will be removed by subsequent washing procedure. Next, 3, 3", 5, 5" tetramethyl-benzidine(TMB), a substrate for HRP is added to each well. The colorimetric reaction is proportional to the B2MG content in each well. The reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at the wavelength of 450nm ± 2nm.
In order to quantify the amount of B2MG present in the sample, this Human B2MG ELISA Kit includes a set of calibration standards (6 standards). The calibration standards should be assayed at the same time as the samples to allow the operator to produce a standard curve of Optical Density (O.D.) versus B2MG concentration (mg/mL). The concentration of B2MG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
CITATIONS
1. Messchendorp AL, Meijer E, Visser FW, Engels GE, Kappert P, Losekoot M, Peters DJM, Gansevoort RT.Rapid Progression of Autosomal Dominant Polycystic Kidney Disease: Urinary Biomarkers as Predictors. Am J Nephro 2019; 50(5):375-385.
Additional
Additional Information
| Product Specificity | Human B2MG ELISA Kit |
|---|---|
| Application | Refer to Insert |
| Size | 96 wells |
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文章原文链接http://www.jbc.org/content/282/23/16776.long
IgM是免疫球蛋白M(Immunoglobulin M，IgM)的缩写。根据结构的不同将免疫球蛋白分为五种，IgM是人的免疫球蛋白之一，其他还有lgA、lgG、IgD和lgE。
问题分析：单纯疱疹是由单纯疱疹病毒感染引起的.单纯疱疹病毒最大的特点是,这种病毒很难被“斩尽杀绝”,总有一少部分病毒在体内潜伏下来,伺机作乱.这些病毒何时再次致病很难预料,一般情况下发热,情绪紧张,月经,妊娠等因素都可能引起发病或复发.发病后即使不经治疗,患者2--4周后也可自愈,只是部分病毒仍然潜伏在体内.目前对这种由单纯疱疹病毒引起的疾病,没有很好的药物治疗,发作时主要使用各种洛韦类抗病毒药,如阿昔洛韦等.主要还是要保持良好的情绪,避免劳累等.
指导建议：IGG和IGM是两种不同的免疫球蛋白，两者产生的先后顺序不一样，一般来说IGM阳性提示在急性感染期，IGG阳 性有可能与以前曾经有过感染有关的。aware可自测不用抽血祝您健康天 猫！
