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Description
Details
Description:Mouse monoclonal antibody to human beta 2 microglobulin (B2MG)
Purification:Protein G affinity purified
Product Type:Primary antibody-Capture antibody in matched antibody pair.
Target Protein:Human beta 2 microglobulin
Immunogen:Highly purified beta 2 microglobulin from human urine
Fusion Myeloma:Sp2/0-Ag14
Species Reacitvity: Human, others not tested
Host / Isotype: Mouse, IgG1 Kappa
Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.2.
Reconstitution:Double distillated water is recommended to reconstitute the antibody.
Storage: Store at -20oC
Research Area:Renal disease, plasma cell cancer
Background:
Human beta 2 microglobulin (B2MG) is a component of major histocompatibility complex 1 (MHC1). It is abundant at the surface of lymphocytes and exists at the surface of all cell types except red cells and platelets. B2MG is normally filtered out of blood through glomeruli, but the process is impaired in patients with renal diseases. Elevated B2MG level is implicated with lymphoma, multiple myeloma, renal diseases, viral infections and auto-immune conditions.
Application:
1. ELISA:In combination with HRP conjugated anti-B2MG mAb clone D3A2 (Cat. No. MO-M40021F), clone D2E9 can be used as capturer in Sandwich ELISA for B2MG detection.
2. Western Blot:Detect 100ng recombinant human B2MG antigen (left lane) and Jurkat cell lysate (middle lane) using anti-human B2MG mAb clone D2E9 at 1mg/mL dilution. The molecular weight of B2MG is about 13-14KD.
3. Fluorescence Flow Cytometry:
Above is the histogram of Hela cells strained with mouse anti-B2MG mAb clone D2E9 and fluorescence labelled secondary antibody. Black line histogram represents Hela cells stained with isotype control, mouse anti-TB 38Kd antigen mAb (MO-I40021D, B12F8) and fluorescence labelled secondary antibody.
4. Immuno-fluorescence staining:
Staining of methanol-fixed Hela cells by Immunocytochemistry: Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D2E9) 1:50 (20ug/mL).
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Human B2MG, D2E9, Capture |
|---|---|
| Application | ELISA, WB, IF, IHC |
| Size | 0.1 mg |
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文章原文链接http://www.jbc.org/content/282/23/16776.long
IgM是免疫球蛋白M(Immunoglobulin M，IgM)的缩写。根据结构的不同将免疫球蛋白分为五种，IgM是人的免疫球蛋白之一，其他还有lgA、lgG、IgD和lgE。
问题分析：单纯疱疹是由单纯疱疹病毒感染引起的.单纯疱疹病毒最大的特点是,这种病毒很难被“斩尽杀绝”,总有一少部分病毒在体内潜伏下来,伺机作乱.这些病毒何时再次致病很难预料,一般情况下发热,情绪紧张,月经,妊娠等因素都可能引起发病或复发.发病后即使不经治疗,患者2--4周后也可自愈,只是部分病毒仍然潜伏在体内.目前对这种由单纯疱疹病毒引起的疾病,没有很好的药物治疗,发作时主要使用各种洛韦类抗病毒药,如阿昔洛韦等.主要还是要保持良好的情绪,避免劳累等.
指导建议：IGG和IGM是两种不同的免疫球蛋白，两者产生的先后顺序不一样，一般来说IGM阳性提示在急性感染期，IGG阳 性有可能与以前曾经有过感染有关的。aware可自测不用抽血祝您健康天 猫！
