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Description
Details
Description:Mouse monoclonal antibody to human beta 2 microglobulin (B2MG)
Purification:Protein G affinity purified
Product Type:Primary antibody-Capture antibody in matched antibody pair.
Target Protein:Human beta 2 microglobulin
Immunogen:Highly purified beta 2 microglobulin from human urine
Fusion Myeloma:Sp2/0-Ag14
Species Reacitvity: Human, others not tested
Host / Isotype: Mouse, IgG1 Kappa
Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.2.
Reconstitution:Double distillated water is recommended to reconstitute the antibody.
Storage: Store at -20oC
Research Area:Renal disease, plasma cell cancer
Background:
Human beta 2 microglobulin (B2MG) is a component of major histocompatibility complex 1 (MHC1). It is abundant at the surface of lymphocytes and exists at the surface of all cell types except red cells and platelets. B2MG is normally filtered out of blood through glomeruli, but the process is impaired in patients with renal diseases. Elevated B2MG level is implicated with lymphoma, multiple myeloma, renal diseases, viral infections and auto-immune conditions.
Application:
1. ELISA:In combination with HRP conjugated anti-B2MG mAb clone D3A2 (Cat. No. MO-M40021F), clone D2E9 can be used as capturer in Sandwich ELISA for B2MG detection.
2. Western Blot:Detect 100ng recombinant human B2MG antigen (left lane) and Jurkat cell lysate (middle lane) using anti-human B2MG mAb clone D2E9 at 1mg/mL dilution. The molecular weight of B2MG is about 13-14KD.
3. Fluorescence Flow Cytometry:
Above is the histogram of Hela cells strained with mouse anti-B2MG mAb clone D2E9 and fluorescence labelled secondary antibody. Black line histogram represents Hela cells stained with isotype control, mouse anti-TB 38Kd antigen mAb (MO-I40021D, B12F8) and fluorescence labelled secondary antibody.
4. Immuno-fluorescence staining:
Staining of methanol-fixed Hela cells by Immunocytochemistry: Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D2E9) 1:50 (20ug/mL).
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Human B2MG, D2E9, Capture |
|---|---|
| Application | ELISA, WB, IF, IHC |
| Size | 0.1 mg |
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IgM是免疫球蛋白M(Immunoglobulin M，IgM)的缩写。根据结构的不同将免疫球蛋白分为五种，IgM是人的免疫球蛋白之一，其他还有lgA、lgG、IgD和lgE。
HSV1型导致的一般都是腰部以上的感染，典型症状是水泡有可能引起脑炎，而且有非活化状态的潜伏。所以我觉得你根本就不用担心这个抗体IgG阳性的化验单。
2-IgM也是一种特异性感染指标，但在人体内持续时间不长（有时是30天更短），往往是传染病的近期感染指标。呈阴性是指：你近期没有感染过结核杆菌。
文章原文链接http://www.jbc.org/content/282/23/16776.long
