![SMOBIO/[DM2360] FluoroBand™ 100 bp+3K Fluorescent DNA Ladder, 500 μl/500 μl/DM2360](images/SMOBIO/image.jpg)
Description
The DM2360 FluoroBand™ 100 bp+3K Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2360 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 3,000 bp
Concentration
56 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light Room temperature for 6 months4°C for 12 months -20°C for 24 months
Specification
Cat. No. | DM2360 |
Series Name | FluoroBand™ |
Product Size | 500 μl |
Size Range | 100 – 3000 bp |
Band Number | 12 |
Tracking Dye | Orange G and Xylene cyanol FF |
Enhanced Band | 500 and 1500 bp |
Manual
Manual_DM2360_FluoroBand™ 100 bp+3K Fluorescent DNA Ladder
SDS
SDS_DM2360
Are the DNA markers/ladders produced by SMOBIO sufficient in quantity?
Yes, all the DNA markers of SMOBIO have been passed in the QC processes including repeated optical density measurements to ensure the quantity of total DNA.
Can I combine non-fluorescent markers and fluorescent loading dye (ex. DL5000) to replace FluoroBand™ fluorescent DNA ladders?
SMOBIO’s fluorescent DNA ladders is better in intensity and accuracy than a fluorescent marker produced by mixing non-fluorescent one with reagents containing fluorescent DNA dyes such as DL5000. In another aspect, DL5000 is designed for quick screening, not intended for preparation of DNA ladders which needs careful calibration. Therefore we suggest using our fluorescent DNA ladders directly.
Will FluoroBand™ fluorescent DNA ladders gradually lose fluorescent intensity?
The fluorescent signals of a fluorescent DNA ladder might be reduced if frequently exposed to light for a long term. Therefore, we suggest keeping fluorescent DNA ladders from exposure to light.
Can fluorescent DNA markers be visualized when illuminated with UV light?
Yes, it is possible to view the fluorescent signals under blue light and UV light.
The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins
Chih-Ying Lin, Lih-Yuan Lin PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971
PMCID: PMC5790263
Transposable elements generate population-specific insertional patterns and allelic variation in genes of wild emmer wheat (Triticum turgidum ssp. dicoccoides)
Katherine Domb, Danielle Keidar, Beery Yaakov, Vadim Khasdan, Khalil Kashkush BMC Plant Biol. 2017; 17: 175. Published online 2017 Oct 27. doi: 10.1186/s12870-017-1134-z
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Azam Amiresmaeili, Samira Roohollahi, Ali Mostafavi, Nayere Askari Res Pharm Sci. 2018 Apr; 13(2): 130–141.
doi: 10.4103/1735-5362.223795
PMCID: PMC5842484
FluoroBand™ DNA Ladder series
ExcelBand™ DNA Ladder series
FluoroDye™ DNA Fluorescent Loading Dye
Excellent for premix with DNA sample
Sensitivity up to 0.14 ng DNA
Safety dye
Convenience - monitor the electrophoresis in real-time
FluoroVue™ Nucleic Acid Gel Stain
Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
B-BOX™ Blue Light LED Epi-illuminator
470 nm long wavelength
Improved cloning efficiency
Compact, light-weight, and portable (less than 1 kg)
Adjustable and removable filter plate allows for gel cutting, visualization, and documentation
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1，IgG抗体是抗体中分子量最小的一种，可通过胎盘输给胎儿，保护了婴儿最初六个月内免受感染。该抗体产生晚，维持时间长，消失慢，浓度高。血中检测到可作为远期感染指标。
2，IgM抗体是抗体中分子量最大的一种，不能通过胎盘输给胎儿。抗体产生最早，一经感染，快速产生，在感染初期抗感染起作用。但维持时间短，消失快。
3，灵长类动物主要有四种免疫球蛋白IgG、IgM、IgA、和IgE。
所有用于检测抗原的免疫学方法经适当改良后，均可用于抗体的检测，如IFA、ELISA、RIA、LA......由于抗原方法的敏感性提高和PCR技术的应用，使得HSV抗体在HSV感染个体中的不均一性和不稳定性影响了这类指标在临床诊断中的意义，但作为一种感染有关指标，在一定的范围和情况下，仍有必要进行检测和深入研究.目前HSV特异性抗体的检测，主要有IgG、IgM和IgA三种。
本试验根据《中国药典》2015年版紫外分光光度法吸收系数法进行制定，本试验方法采用的方法学验证内容如下：
1.线性及范围
取IgG标准品配制5个不同浓度被测样品，并进行测定，绘制标准曲线，得到回归方程及范围。
2.准确度
已回收率对准确度进行验证。以已知含量的IgG标准品配制供试品，配制三个浓度，每个浓度平均测定三次。计算回收率。
3.精密度
IgG标准品配制供试品，平行测定六次。
4.溶液稳定性试验
至少持续2h的溶液稳定性考察。
5.干扰试验
空白溶液中加入辅料，在280nm处测定吸收，确定辅料是否对样品的吸收造成干扰。
以上，请大神指正。
补充问题，在专属性验证上，仅做辅料干扰是不是太少了？是否再进行强降解实验呢？因为其中包含了蛋白质A、外源性DNA及宿主细胞蛋白残留。我是否应该针对这三种杂质进行专属性试验？该如何进行呢？
问题分析：单纯疱疹是由单纯疱疹病毒感染引起的.单纯疱疹病毒最大的特点是,这种病毒很难被“斩尽杀绝”,总有一少部分病毒在体内潜伏下来,伺机作乱.这些病毒何时再次致病很难预料,一般情况下发热,情绪紧张,月经,妊娠等因素都可能引起发病或复发.发病后即使不经治疗,患者2--4周后也可自愈,只是部分病毒仍然潜伏在体内.目前对这种由单纯疱疹病毒引起的疾病,没有很好的药物治疗,发作时主要使用各种洛韦类抗病毒药,如阿昔洛韦等.主要还是要保持良好的情绪,避免劳累等.
指导建议：IGG和IGM是两种不同的免疫球蛋白，两者产生的先后顺序不一样，一般来说IGM阳性提示在急性感染期，IGG阳 性有可能与以前曾经有过感染有关的。aware可自测不用抽血祝您健康天 猫！
2-IgM也是一种特异性感染指标，但在人体内持续时间不长（有时是30天更短），往往是传染病的近期感染指标。呈阴性是指：你近期没有感染过结核杆菌。
HSV1型导致的一般都是腰部以上的感染，典型症状是水泡有可能引起脑炎，而且有非活化状态的潜伏。所以我觉得你根本就不用担心这个抗体IgG阳性的化验单。
